Yellow fever virus NS2B-NS3 protease: Charged-to-alanine mutagenesis and deletion analysis define regions important for protease complex formation and function

Virology

Volumn 275, Issue 2, 2000, Pages 335-347

  Droll, Deborah A ^a   Murthy, H M Krishna ^b   Chambers, Thomas J ^a  

^a SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEIN; PROTEINASE; PROTEINASE NS2B; PROTEINASE NS3; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CELLULAR DISTRIBUTION; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; GENE CLUSTER; GENE DELETION; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; SURFACE CHARGE; YELLOW FEVER FLAVIVIRUS;

EID: 0034734773     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.2000.0488     Document Type: Article

References (50)

1. NS2B-3 proteinase-mediated processing in the yellow fever virus structural region: In vitro and in vivo studies
2. Dengue 2 virus NS2B and NS3 form a stable complex that can cleave NS3 within the helicase domain
3. Complex formation between the NS3 serine type proteinase of the hepatitis C virus and NS4A and its importance for polyprotein maturation
4. Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses
5. Homology model of the dengue 2 virus NS3 protease: Putative interactions with both substrate and NS2B cofactor
6. Enhancement of hepatitis C virus NS3 proteinase activity by association with NS4A-specific synthetic peptides: Identification of sequence and critical residues of NS4A for the cofactor activity
7. Cleavage of the dengue virus polyprotein at the NS3/NS4A and NS4B/NS5 junctions is mediated by viral protease NS2B-NS3, whereas NS4A/NS4B may be processed by a cellular protease
8. Production of yellow fever virus proteins in infected cells: Identification of discrete polyprotein species and analysis of cleavage kinetics using region-specific polyclonal antisera
9. Evidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein
10. Processing of the yellow fever virus nonstructural polyprotein: A catalytically active NS3 proteinase domain and NS2B are required for cleavages at dibasic sites
11. Mutagenesis of the yellow fever virus NS2B protein: Effects on proteolytic processing, NS2B-NS3 complex formation and viral replication
12. Mutagenesis of the yellow fever virus NS2B/3 cleavage site; Determinants of cleavage site specificity and effects of mutations on polyprotein processing and viral replication
13. Cotranslational membrane insertion of the serine proteinase precursor NS2B-NS3(Pro) of dengue virus type 2 is required for efficient in vitro processing and is mediated through the hydrophobic regions of NS2B
14. Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis
15. Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins
16. An amino-terminal domain of the hepatitis C virus NS3 protease is essential for interaction with NS4A
17. Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins
18. Deletion analysis of dengue virus type 4 nonstructural protein NS2B: Identification of a domain required for NS2B-NS3 protease activity
19. Identification of functional residues in proteins by charged-to-alanine scanning mutagenesis
20. N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases
21. Analysis of a temperature-sensitive vaccinia virus mutant in the viral mRNA capping enzyme isolated by clustered charge-to-alanine mutagenesis and transient dominant selection
22. Crystal structure of the hepatitis C virus NS3 protease domain complexed with a synthetic NS4A cofactor peptide
23. In vitro studies on the activation of the hepatitis C virus NS3 proteinase by the NS4A cofactor
24. Rapid and efficient site-specific mutagenesis without phenotypic selection
25. Cleavage at a novel site in the NS4A region by the yellow fever virus NS2B-3 proteinase is a prerequisite for processing at the downstream 4A/4B signalase site
26. Mutagenesis of conserved residues at the yellow fever 3/4A and 4B/5 dibasic cleavage sites: Effects on cleavage efficiency and polyprotein processing
27. A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine proteinase complex in vivo and in vitro
28. Proteolytic processing of a Murray Valley encephalitis virus nonstructural polyprotein segment containing the viral proteinase: Accumulation of a NS3-4A precursor which requires mature NS3 for efficient processing
29. Flavivirus premembrane protein cleavage and spike heterodimer secretion require the function of the viral proteinase NS3
30. The crystal structure of hepatitis C virus NS3 proteinase reveals a trypsin-like fold and a structural zinc binding site
31. The conformation of hepatitis C virus NS3 protein-ase with and without NS4A: A structural basis for the activation of the enzyme by its cofactor
32. Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4a
33. Dengue virus NS3 serine protease; Crystal structure and insights into interaction of the active site with substrates by molecular modeling and structural analysis of mutational effects
34. Genetic analysis of the yellow fever virus NS1 protein: Identification of a temperature-sensitive mutation which blocks RNA accumulation
35. Mutagenesis of the yellow fever virus NS2A/2B cleavage site: Effects on proteolytic processing, viral replication and evidence for alternative processing of the NS2A protein
36. In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3
37. Processing of nonstructural proteins NS4A and NS4B of dengue 2 virus in vitro and in vivo
38. Virus-encoded proteinases of the Flaviviridae
39. The N-terminal region of hepatitis C virus nonstructural protein 3 (NS3), is essential for stable complex formation with NS4A
40. Hepatitis C virus-encoded nonstructural protein NS4A has versatile functions in viral protein processing
41. Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes
42. Mutagenesis of the NS3 protease of dengue virus type 2
43. In vitro synthesis of West Nile virus proteins indicates that the amino terminal segment of the NS3 protein contains the active centre of the protease which cleaves the viral polyprotein after multiple basic amino acids
44. Ultrastructure of Kunjin virus-infected cells: Colocalization of NS1 and NS3, with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures
45. Nascent flavivirus RNA colocalized in situ with double-stranded RNA in stable replication complexes
46. Processing of the intracellular form of the West Nile virus capsid protein by the viral NS2B-NS3 protease: An in vitro study
47. Formation of the flavi-virus envelope: Role of the viral NS2B-NS3 protease
48. Complex of NS3 protease and NS4a peptide of BK strain hepatitis C virus: A 2.2 angstrom resolution structure in a hexagonal crystal form
49. Processing and localization of dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5