Enzymatic characterization and homology model of a catalytically active recombinant West Nile Virus NS3 protease

Journal of Biological Chemistry

Volumn 279, Issue 47, 2004, Pages 48535-48542

  Nall, Tessa A ^a   Chappell, Keith J ^a   Stoermer, Martin J ^a   Fang, Ning Xia ^a   Tyndall, Joel D A ^a,b   Young, Paul R ^a   Fairlie, David P ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; CRYSTAL STRUCTURE; DISEASES; ENZYME INHIBITION; GLYCEROL; NEUROLOGY;

ENZYMATIC CHARACTERIZATION; PARA-NITIROANILIDE (PNA); VIRAL POLYPEPTIDE PRECURSOR; WEST NILE VIRUS (WNV);

ENZYME KINETICS;

GLYCEROL; HEXAPEPTIDE; NONAPEPTIDE; PROTEINASE; RECOMBINANT ENZYME; VIRUS ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; DENGUE VIRUS; ENZYME ASSAY; ENZYME STRUCTURE; HEPATITIS C VIRUS; IC 50; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; WEST NILE FLAVIVIRUS;

AMINO ACID SEQUENCE; ANTIVIRAL AGENTS; BINDING SITES; CATALYSIS; CYTOPLASM; DENGUE VIRUS; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCEROL; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; INHIBITORY CONCENTRATION 50; KINETICS; LIGANDS; MODELS, CHEMICAL; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN CONFORMATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; RNA HELICASES; SEQUENCE HOMOLOGY, AMINO ACID; SERINE ENDOPEPTIDASES; SODIUM CHLORIDE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBSTRATE SPECIFICITY; VIRAL NONSTRUCTURAL PROTEINS; WEST NILE VIRUS;

ANIMALIA; DENGUE VIRUS; FLAVIVIRUS; HEPATITIS C VIRUS; RNA VIRUSES; WEST NILE VIRUS;

EID: 10344248922     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M406810200     Document Type: Article

References (32)

1. Brinton, M. A. (2002) Annu. Rev. Microbiol. 56, 371-402
2. Hayes, C. G. (2001) Ann. N. Y. Acad. Sci. 951, 25-37
3. Lanciotti, R. S., Roehrig, J. T., Deubel, V., Smith, J., Parker, M., Steele, K., Crise, B., Volpe, K. E., Crabtree, M. B., Scherret, J. H., Hall, R. A., MacKenzie, J. S., Cropp, C. B., Panigrahy, B., Ostlund, E., Schmitt, B., Malkinson, M., Banet, C., Weissman, J., Komar, N., Savage, H. M., Stone, W., McNamara, T., and Gubler, D. J. (1999) Science 286, 2333-2337
4. Teo, K. F., and Wright, P. J. (1997) J. Gen. Virol. 78, 337-341
5. Buckley, A., Dawson, A., Moss, S. R., Hinsley, S. A., Bellamy, P. E., and Gould, E. A. (2003) J. Gen. Virol. 84, 2807-2817
6. Leung, D., Schroder, K., White, H., Fang, N. X., Stoermer, M. J., Abbenante, G., Martin, J. L., Young, P. R., and Fairlie, D. P. (2001) J. Biol. Chem. 276, 45762-45771
7. Clum, S., Ebner, K. E., and Padmanabhan, R. (1997) J. Biol. Chem. 272, 30715-30723
8. Love, R. A., Parge, H. E., Wickersham, J. A., Hostomsky, Z., Habuka, N., Moomaw, E. W., Adachi, T., Margosiak, S., Dagostino, E., and Hostomska, Z. (1998) Clin. Diagn. Virol. 10, 151-156
9. Murthy, H. M., Clum, S., and Padmanabhan, R. (1999) J. Biol. Chem. 274, 5573-5580
10. Gulick, R. M. (2003) Clin. Microbiol. Infect. 9, 186-193
11. Rutenber, E., Fauman, E. B., Keenan, R. J., Fong, S., Furth, P. S., Ortiz de Montellano, P. R., Meng, E., Kuntz, I. D., DeCamp, D. L., Salto, R., Rose, J. R., Craik, C. S., and Stroud, R. M. (1993) J. Biol. Chem. 268, 15343-15346
12. Lin, C., Lin, K., Luong, Y. P., Rao, B. G., Wei, Y. Y., Brennan, D. L., Fulghum, J. R., Hsiao, H. M., Ma, S., Maxwell, J. P., Cottrell, K. M., Perni, R. B., Gates, C. A., and Kwong, A. D. (2004) J. Biol. Chem. 279, 17508-17514
13. Wuthrich, K. (1986) NMR of Proteins and Nucleic Acids, Wiley-Interscience, New York
14. Amberg, S. M., and Rice, C. M. (1999) J. Virol. 73, 8083-8094
15. Abbenante, G., Leung, D., Bond, T., and Fairlie, D. P. (2001) LIPS 7, 347-351
16. Siev, D. V., and Semple, J. E. (2000) Org. Lett. 2, 19-22
17. Gussio, R., Pattabiraman, N., Zaharevitz, D. W., Kellogg, G. E., Topol, I. A., Rice, W. G., Schaeffer, C. A., Erickson, J. W., and Burt, S. K. (1996) J. Med. Chem. 39, 1645-1650
18. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) Nucleic Acids Res. 22, 4673-4680
19. Jones, D. T. (1999) J. Mol. Biol. 292, 193-202
20. Hicks, R. P., Mones, E., Kim, H., Koser, B. W., Nichols, D. A., and Bhattacharjee, A. K. (2003) Biopolymers 68, 459-470
21. Jones, G., Willett, P., and Glen, R. C. (1995) J. Mol. Biol. 245, 43-53
22. Fairlie, D. P., Tyndall, J. D. A., Reid, R. C., Wong, A. K., Abbenante, G., Scanlon, M. J., March, D. R., Bergman, D. A., Chai, C. L. L., Burkett, B. A. (2000) J. Med. Chem. 43, 1271-1281
23. Leung, D., Abbenante, G., Fairlie, D. P. (2000) J. Med. Chem. 43, 305-341
24. Stocks, C. E., and Lobigs, M. (1998) J. Virol. 72, 2141-2149
25. Brinkworth, R. I., Fairlie, D. P., Leung, D., and Young, P. R. (1999) J. Gen. Virol. 80, 1167-1177
26. Arakaki, T. L., Fang, N. X., Fairlie, D. P., Young, P. R., and Martin, J. L. (2002) Protein Expr. Purif. 25, 241-247
27. Tyndall, J. D. A., and Fairlie, D. P. (1999) J. Mol. Recognit. 12, 363-370
28. Schultz, R. M., Varma-Nelsons, P., Ortiz, R., Kozlowskin, K. A., Orawski, A. T., Pagast, P., and Frankfater, A. (1989) J. Biol. Chem. 264, 1497.
29. Campbell, G. L., Marfin, A. A., Lanciotti, R. S., and Gubler, D. J. (2002) Lancet Infect. Dis. 2, 519-529
30. Glenn, M. P., and Fairlie, D. P. (2002) Mini Rev. Med. Chem. 2, 433-445
31. Loughlin, W. A., Tyndall, J. D. A., Kelso, M. K., and Fairlie, D. P. (2004) Chem. Reviews, in press
32. Falgout, B., Miller, R. H., and Lai, C. J. (1993) J. Virol. 67, 2034-2042