Dihydroceramide desaturase knockdown impacts sphingolipids and apoptosis after photodamage in human head and neck squamous carcinoma cells

Anticancer Research

Volumn 33, Issue 1, 2013, Pages 77-84

  Breen, Paul ^a   Joseph, Nicholas ^a   Thompson, Kyle ^a   Kraveka, Jacqueline M ^b   Gudz, Tatyana I ^c,d   Li, Li ^b   Rahmaniyan, Mehrdad ^b   Bielawski, Jacek ^d   Pierce, Jason S ^d   Van Buren, Eric ^a   Bhatti, Gaurav ^a   Separovic, Duska ^a  

^a WAYNE STATE UNIVERSITY   (United States)

^b Department of Pediatrics Division of Hematology Oncology ^*  (United States)

^c VETERANS AFFAIRS MEDICAL CENTER   (United States)

^d MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

Apoptosis; Ceramide; Dihydroceramide; Dihydroceramide desaturase; HNSCC; PDT; Sphingolipids; UM SCC 22A cells

Indexed keywords

CASPASE 3; CERAMIDE DERIVATIVE; DIHYDROCERAMIDE DESATURASE; SPHINGANINE; SPHINGOLIPID; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL VIABILITY; CHANNEL GATING; CONTROLLED STUDY; DEPOLARIZATION; DOWN REGULATION; HEAD AND NECK SQUAMOUS CELL CARCINOMA; HUMAN; HUMAN CELL; MITOCHONDRIAL MEMBRANE POTENTIAL; PHOTODYNAMIC THERAPY; PRIORITY JOURNAL; PROTEIN EXPRESSION;

APOPTOSIS; CARCINOMA, SQUAMOUS CELL; CELL LINE, TUMOR; CERAMIDES; GENE EXPRESSION REGULATION, NEOPLASTIC; HEAD AND NECK NEOPLASMS; HUMANS; INDOLES; MOLECULAR TARGETED THERAPY; OXIDOREDUCTASES; PHOTOCHEMOTHERAPY; RNA, SMALL INTERFERING; SPHINGOLIPIDS;

EID: 84873030975     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Bartke N and Hannun YA: Bioactive sphingolipids: Metabolism and function. J Lipid Res 50(Suppl): S91-96, 2009.
2. Fabrias G, Munoz-Olaya J, Cingolani F, Signorelli P, Casas J, Gagliostro V and Ghidoni R: Dihydroceramide desaturase and dihydrosphingolipids: Debutant players in the sphingolipid arena. Prog Lipid Res 51: 82-94, 2012.
3. Zheng W, Kollmeyer J, Symolon H, Momin A, Munter E, Wang E, Kelly S, Allegood JC, Liu Y, Peng Q, Ramaraju H, Sullards MC, Cabot M and Merrill AH Jr.: Ceramides and other bioactive sphingolipid backbones in health and disease: Lipidomic analysis, metabolism and roles in membrane structure, dynamics, signaling and autophagy. Biochim Biophys Acta 1758: 1864-1884, 2006.
4. Stiban J, Fistere D and Colombini M: Dihydroceramide hinders ceramide channel formation: Implications on apoptosis. Apoptosis 11: 773-780, 2006.
5. Kraveka JM, Li L, Szulc ZM, Bielawski J, Ogretmen B, Hannun YA, Obeid LM and Bielawska A: Involvement of dihydroceramide desaturase in cell cycle progression in human neuroblastoma cells. J Biol Chem 282: 16718-16728, 2007.
6. Rahmaniyan M, Curley RW, Jr, Obeid LM, Hannun YA and Kraveka JM: Identification of dihydroceramide desaturase as a direct in vitro target for fenretinide. J Biol Chem 286: 24754-24764, 2011.
7. Dolgachev V, Farooqui MS, Kulaeva OI, Tainsky MA, Nagy B, Hanada K and Separovic D: De novo ceramide accumulation due to inhibition of its conversion to complex sphingolipids in apoptotic photosensitized cells. J Biol Chem 279: 23238-23249, 2004.
8. Dolgachev V, Nagy B, Taffe B, Hanada K and Separovic D: Reactive oxygen species generation is independent of de novo sphingolipids in apoptotic photosensitized cells. Exp Cell Res 288: 425-436, 2003.
9. Wispriyono B, Schmelz E, Pelayo H, Hanada K and Separovic D: A role for the de novo sphingolipids in apoptosis of photosensitized cells. Exp Cell Res 279: 153-165, 2002.
10. Agostinis P, Berg K, Cengel KA, Foster TH, Girotti AW, Gollnick SO, Hahn SM, Hamblin MR, Juzeniene A, Kessel D, Korbelik M, Moan J, Mroz P, Nowis D, Piette J, Wilson BC and Golab J: Photodynamic therapy of cancer: An update. CA Cancer J Clin 61: 250-281, 2011.
11. Oleinick N, Morris R and Nieminen A-L. Photodynamic Therapy-Induced Apoptosis. In: Apoptosis, Senescence and Cancer Gewirtz DA, Holt SE and Grant S (eds.). Totowa: Humana Press Inc. pp. 557-578, 2007.
12. Separovic D, Breen P, Joseph N, Bielawski J, Pierce JS, Van Buren E and Gudz TI: Ceramide synthase 6 knockdown suppresses apoptosis after photodynamic therapy in human head and neck squamous carcinoma cells. Anticancer Res 32: 753-760, 2012.
13. Separovic D, Breen P, Joseph N, Bielawski J, Pierce JS, Van Buren E and Gudz TI: siRNA-mediated down-regulation of ceramide synthase 1 leads to apoptotic resistance in human head and neck squamous carcinoma cells after photodynamic therapy. Anticancer Res 32: 2479-2485, 2012.
14. Brenner JC, Graham MP, Kumar B, Saunders LM, Kupfer R, Lyons RH, Bradford CR and Carey TE: Genotyping of 73 UMSCC head and neck squamous cell carcinoma cell lines. Head Neck 32: 417-426, 2010.
15. Zhu Z, Xu X, Yu Y, Graham M, Prince ME, Carey TE and Sun D: Silencing heat-shock protein 27 decreases metastatic behavior of human head and neck squamous cell cancer cells in vitro. Mol Pharm 7: 1283-1290, 2010.
16. Separovic D, Semaan L, Tarca AL, Awad Maitah MY, Hanada K, Bielawski J, Villani M and Luberto C: Suppression of sphingomyelin synthase 1 by small interference RNA is associated with enhanced ceramide production and apoptosis after photodamage. Exp Cell Res 314: 1860-1868, 2008.
17. Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: e45, 2001.
18. Separovic D, Kelekar A, Nayak AK, Tarca AL, Hanada K, Pierce JS and Bielawski J: Increased ceramide accumulation correlates with down-regulation of the autophagy protein ATG-7 in MCF-7 cells sensitized to photodamage. Arch Biochem Biophys 494: 101-105, 2010.
19. Separovic D, Saad ZH, Edwin EA, Bielawski J, Pierce JS, Van Buren E and Bielawska A: C16-ceramide analog combined with Pc 4 photodynamic therapy evokes enhanced total ceramide accumulation, promotion of DEVDase activation in the absence of apoptosis, and augmented overall cell killing. J Lipids 2011: 1-9, 2011.
20. Separovic D, Mann KJ and Oleinick NL: Association of ceramide accumulation with photodynamic treatment-induced cell death. Photochem Photobiol 68: 101-109, 1998.
21. Lam M, Oleinick NL and Nieminen AL: Photodynamic therapyinduced apoptosis in epidermoid carcinoma cells. Reactive oxygen species and mitochondrial inner membrane permeabilization. J Biol Chem 276: 47379-47386, 2001.
22. Devlin CM, Lahm T, Hubbard WC, Van Demark M, Wang KC, Wu X, Bielawska A, Obeid LM, Ivan M and Petrache I: Dihydroceramide-based response to hypoxia. J Biol Chem 286: 38069-38078, 2011.
23. Ganesan V, Perera MN, Colombini D, Datskovskiy D, Chadha K and Colombini M: Ceramide and activated Bax act synergistically to permeabilize the mitochondrial outer membrane. Apoptosis 15: 553-562, 2010.
24. Siskind LJ, Kolesnick RN and Colombini M: Ceramide channels increase the permeability of the mitochondrial outer membrane to small proteins. J Biol Chem 277: 26796-26803, 2002.
25. Chiu SM, Xue LY, Usuda J, Azizuddin K and Oleinick NL: Bax is essential for mitochondrion-mediated apoptosis but not for cell death caused by photodynamic therapy. Br J Cancer 89: 1590-1597, 2003.
26. Nagy B, Chiu S-M and Separovic D: Fumonisin B1 does not prevent apoptosis in A431 human epidermoid carcinoma cells after photosensitization with phthalocyanine 4. J Photochem Photobiol B: Biology 57: 132-141, 2000.
27. Idkowiak-Baldys J, Apraiz A, Li L, Rahmaniyan M, Clarke CJ, Kraveka JM, Asumendi A and Hannun YA: Dihydroceramide desaturase activity is modulated by oxidative stress. Biochem J 427: 265-274, 2010.