Directed evolution of g-protein-coupled receptors for high functional expression and detergent stability

Methods in Enzymology

Volumn 520, Issue , 2013, Pages 67-97

  Schlinkmann, Karola M ^a   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Directed evolution; Expression; Keywords; Membrane proteins; Mutagenesis; Randomization; Screening; Selection; Stability

Indexed keywords

DETERGENT; G PROTEIN COUPLED RECEPTOR;

AMINO ACID SEQUENCE; ARTICLE; CLONING; DNA SHUFFLING; ESCHERICHIA COLI; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC VARIABILITY; HIGH THROUGHPUT SCREENING; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS;

EID: 84872556402     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-391861-1.00004-6     Document Type: Chapter

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