Blinking effect and the use of quantum dots in single molecule spectroscopy

Biochemical and Biophysical Research Communications

Volumn 430, Issue 1, 2013, Pages 260-264

  Rombach Riegraf, Verena ^a   Oswald, Peter ^a   Bienert, Roland ^a   Petersen, Jan ^a   Domingo, M P ^b   Pardo, Julian ^c,d   Graber P ^a   Galvez, E M ^b,d  

^a UNIVERSITY OF FREIBURG   (Germany)

^b INSTITUTO DE CARBOQUÍMICA   (Spain)

^c UNIVERSITY OF ZARAGOZA   (Spain)

^d FUNDACIÓN ARAID   (Spain)

Author keywords

Blinking; Cysteine; Protein; Quantum dot; Single molecule fluorescence

Indexed keywords

APROTININ; BOVINE SERUM ALBUMIN; CYSTEINE; FETUIN; IMMUNOGLOBULIN G; NANOCRYSTAL; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; QUANTUM DOT;

ARTICLE; BINDING SITE; CHEMICAL ANALYSIS; CHEMICAL COMPOSITION; DYNAMICS; EXPERIMENTAL STUDY; FLUORESCENCE SPECTROSCOPY; MOLECULAR WEIGHT; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; SEMICONDUCTOR; SURFACE PROPERTY;

ANIMALS; CATTLE; CYSTEINE; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; PROTEIN INTERACTION MAPPING; QUANTUM DOTS; SPECTROMETRY, FLUORESCENCE;

EID: 84872406729     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.10.140     Document Type: Article

References (73)

1. Bawendi M.G., Wilson W.L., Rothberg L., Carroll P.J., Jedju T.M., Steigerwald M.L., Brus L.E. Electronic structure and photoexcited-carrier dynamics in nanometer-size cadmium selenide clusters. Phys. Rev. Lett. 1990, 65:1623-1626.
2. Mews A., Kadavanich A.V., Banin U., Alivisatos A.P. Structural and spectroscopic investigations of CdS/HgS/CdS quantum-dot quantum wells. Phys. Rev. B 1996, 53(20):13242-13245.
3. Chan W.C.W., Nie S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 1998, 281:2016-2028.
4. Bruchez M., Moronne J.M., Gin P., Weiss S., Alivisatos A.P. Semiconductor nanocrystals as fluorescent biological labels. Science 1998, 281(281):2013-2018.
5. Mattoussi H., Mauro J.M., Goldman E.R., Anderson G.P., Sundar V.C., Mikulec F.V., Bawendi M.G. Self-assembly of CdSe-ZnS quantum dot bioconjugates using an engineered recombinant protein. J. Am. Chem. Soc. 2000, 122:12142-12150.
6. Dubertret B., Skourides P., Norris D.J., Noireaux V., Brivanlou A.H., Libchaber A. In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science 2002, 298:1759-1761.
7. Wu X.Y., Liu H.J., Liu J.Q. Immunofluorescent labelling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nat. Biotechnol. 2003, 21:41-46.
8. Jaiswal J.K., Mattoussi H., Mauro J.M., Simon S.M. Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat. Biotechnol. 2003, 21:47-51.
9. Frasco M.F., Chaniotakis N. Semiconductor quantum dots in chemical sensors and biosensors. Sensors 2009, 9(9):7266-7286.
10. Nelson S.R., Ali M.Y., Washaw D.M. Quantum dot labelling strategies to characterize single molecule motors. Methods Mol. Biol. 2011, 778:1111-1121.
11. Chu V.H., Nghiem T.H.L., Le T.H., Vu D.L., Tran H.N., Vu T.K.L. Synthesis and optical properties of water soluble CdSe/CdS quantum dots for biological applications. Advances in natural sciences: nanoscience and nanotechnology 2012, 3(2):250171-250177.
12. Mattoussi H., Palui G., Na H.B. Luminescent quantum dots as platforms for probing in vitro and in vivo biological processes. Adv. Drug Deliv. Rev. 2012, 64(2):138-166.
13. Singh G., Zaidi N.H., Soni U., Gautam M., Jackeray R., Singh H., Sapra S. Detection of bioconjugated quantum dots passivated with different ligands for bio-applications. J. Nanosci. Nanotechnol. 2011, 11(5):3834-3842.
14. Chang Y.P., Pinaud F., Antelman J., Weiss S. Tracking bio-molecules in live cells using quantum dots. J. Biophotonics 2008, 1(4):287-298.
15. Toulme E., Khakh B.S. Imaging P2X4 receptor lateral mobility in microglia: regulation by calcium and p38MAPK. J. Biol. Chem. 2012, 287(18):14734-14748.
16. Xue M., Wang X., Duan L., Gao W., Ji L., Tang B. A new nanoprobe based on FRET between functional quantum dots and gold nanoparticles for fluoride anion and its applications for biological imaging. Biosens. Bioelectron. 2012, 36(1):168-173.
17. Chakraborty S., Gogoi M., Kalita E., Deb P. Multifunctional high luminescent biocompatible CdTe quantum dot fluorophores for bioimaging applications. Int. J. Nanosci 2011, 10(4&5):1191-1195.
18. Roy M., Wilson B.C. Molecular imaging with targeted quantum dot bioconjugates: the need for contrast optimisation studies. Int. J. Nanotechnol. 2009, 6(5/6):442-455.
19. Arya H., Kaul Z., Wadhwa R., Taira K., Hirano T., Kaul S.C. Quantum dots in bio-imaging: revolution by the small. Biochem. Biophys. Res. Commun. 2005, 329(4):1173-1177.
20. Gálvez E.M., Zimmermann B., Rombach-Riegraf V., Bienert R., Gräber P. Fluorescence resonance energy transfer in single enzyme molecules with a quantum dot as donor. Eur. Biophys. J. 2008, 37:1367-1371.
21. Gálvez E.M., Düser M., Börsch M., Wrachtrup J., Gräber P. Quantum dots for single-pair fluorescence resonance energy transfer in membrane integrated EF0F1. Biochem. Soc. Trans. 2008, 36:1017-1021.
22. Hohng S., Ha T. Single-molecule quantum-dot fluorescence resonance energy transfer. Chem. Phys. Chem. 2005, 6:956-960.
23. Nirmal M., Dabbousi B.O., Bawendi M.G., Macklin J.J., Trautman J.K., Harris T.D., Brus L.E. Fluorescence intermittency in single cadmium selenide nanocrystals. Nature 1996, 383:802-804.
24. Kuno M., Fromm D.P., Hamann H.F., Gallagher A., Nesbitt D.J. Non exponential 'blinking' kinetics of single CdSe quantum dots: a universal power law behaviour. J. Chem. Phys. 2000, 112:3117-3120.
25. Shimizu K.T., Neuhauser R.G., Leatherdale C.A., Empedocles S.A., Woo W.K., Bawendi M.G. Blinking statistics in single semiconductor nanocrystal quantum dots. Phys. Rev. B 2001, 63:205316.
26. Fisher B.R., Eisler H.J., Stott N.E., Bawendi M.G. Emission intensity dependence and single-exponential behavior in single colloidal quantum dot fluorescence lifetimes. J. Phys. Chem. B 2004, 108:143-148.
27. Brokmann X., Hermier J.P., Messin G., Desbiolles P., Bouchaud J.P., Dahan M. Statistical aging and nonergodicity in the fluorescence of single nanocrystals. Phys. Rev. Lett. 2003, 90:120601.
28. Pelton M., Grier D.G., Guyot-Sionnest P. Characterizing quantum-dot blinking using noise power spectra. Appl. Phys. Lett. 2004, 85:819-821.
29. Verberk R., van Oijen A.M., Orrit M. Simple model for the power-law blinking of single semiconductor nanocrystals. Phys. Rev. B 2002, 66:233202.
30. Schlegel G., Bohnenberger J., Potapova I., Mews A. Fluorescence decay time of single semiconductor nanocrystals. Phys. Rev. Lett. 2003, 88:137401.
31. Verberk R., Orrit M. Photon statistics in the fluorescence of single molecules and nanocrystals: Correlation functions versus distributions of on- and off-times. J. Chem. Phys. 2003, 119:2214-2222.
32. Kuno M., Fromm D.P., Hamann H.F., Gallagher A., Nesbitt D.J. 'On'/'off' fluorescence intermittency of single semiconductor quantum dots. J. Chem. Phys. 2001, 115:1028-1040.
33. Shimizu K.T., Woo W.K., Fisher B.R., Eisler H.J., Bawendi M.G. Surface-enhanced emission from single semiconductor nanocrystals. Phys. Rev. Lett. 2002, 89:117401.
34. Chung I.H., Bawendi M.G. Relationship between single quantumdot intermittency and fluorescence intensity decays from collections of dots. Phys. Rev. B 2004, 70:165304.
35. Chung I., Witkoskie J.B., Cao J., Bawendi M.G. Description of the fluorescence intensity time trace of collections of CdSe nanocrystal quantum dots based on single quantum dot fluorescence blinking statistics. Phys. Rev. E 2006, 73:011106.
36. Kuno M., Fromm D.P., Johnson S.T., Gallagher A, Nesbitt D.J. Modeling distributed kinetics in isolated semiconductor quantum dots. Phys. Rev. B 2003, 67:125304.
37. Messin G., Hermier J.P., Giacobino E., Desbiolles P., Dahan M. Bunching and antibunching in the fluorescence of semiconductor nanocrystals. Opt. Lett. 2001, 26:1891-1893.
38. Issac A., von Borczyskowski C., Cichos F. Correlation between photoluminescence intermittency of CdSe quantum dots and self-trapped states in dielectric media. Phys. Rev. B 2005, 71:161302.
39. Biju V., Makita Y., Nagase T., Yamaoka Y., Yokoyama H., Baba Y., Ishikawa H. Subsecond luminescence intensity fluctuations of single CdSe quantum dots. J. Phys. Chem. B 2005, 109:14350-14355.
40. Tang J., Marcus R.A. Diffusion-controlled electron transfer processes and power-law statistics of fluorescence intermittency of nanoparticles. Phys. Rev. Lett. 2005, 95:107401-107404.
41. Efros A.L., Rosen M. Random telegraph signal in the photoluminescence intensity of a single quantum dot. Phys. Rev. Lett. 1997, 78:1110-1113.
42. Tang J., Marcus R.A. Mechanisms of fluorescence blinking in semiconductor nanocrystal quantum dots. J. Chem. Phys. 2005, 123:054704.
43. Margolin G., Barkai E. Aging correlation functions for blinking nanocrystals, and other on-off stochastic processes. J. Chem. Phys. 2004, 121:1566-1577.
44. Margolin G., Protasenko G.V., Kuno M., Barkai E. Power law blinking quantum dots: stochastic and physical models. Adv. Chem. Phys. 2006, 133:327-356.
45. Tang J., Marcus R.A. Diffusion-controlled electron transfer processes and powerlaw statistics of fluorescence intermittency of nanoparticles. Phys. Rev. Lett. 2005, 95:107401.
46. Frantsuzov P.A., Marcus R.A. Explanation of quantum dot blinking without the long-lived trap hypothesis. Phys. Rev. B 2005, 72:155321.
47. Margolin G., Barkai E. Nonegrodicity of blinking nanocrystals and other levywalk processes. Phys. Rev. Lett. 2005, 94:080601.
48. Bianco S., Grigolini P., Paradisi P. Fluorescence intermittency in blinking quantum dots: renewal or slow modulation?. J. Chem. Phys. 2005, 123:174704.
49. Klimov V.I., Mikhailovsky A.A., McBranch D.W., Leatherdale C.A., Bawendi M.G. Quantization of multiparticle Auger rates in semiconductor quantum dots. Science 2000, 287:1011-1013.
50. Zhao J., Nair G., Fisher B.R., Bawendi M.G. Challenge to the charging model of semiconductor-nanocrystal fluorescence intermittency from off-state quantum yields and multiexciton blinking. Phys. Rev. Lett. 2010, 104:157403.
51. Rosen S., Schwartz O., Oron D. Transient fluorescence of the off state in blinking CdSe/CdS/ZnS semiconductor nanocrystals is not governed by auger recombination. Phys. Rev. Lett. 2010, 104(104):157404.
52. Galland C., Ghosh Y., Steinbrück A., Hollingsworth J.A., Htoon H., Klimov V.I. Lifetime blinking in nonblinking nanocrystal quantum dots. Nat. Commun. 2012, 3:908.
53. Galland C., Ghosh Y., Steinbrück A., Sykora J.A.M., Hollingsworth, Klimov V.I., Htoon H. Two types of luminescence blinking revealed by spectroelectrochemistry of single quantum dots. Nature 2011, 479(7372):3-7.
54. Heuff R.F., Swift J.L., Cramb D.T. Fluorescence correlation spectroscopy using quantum dots: advances, challenges and opportunities. Phys. Chem. Chem. Phys. 2007, 9(16):1870-1880.
55. Chien F.C., Kuo C.W., Chen P. Localization imaging using blinking quantum dots. Analyst 2011, 136(8):1608-1613.
56. Watanabe T.M., Fukui S., Jin T., Fujii F., Yanagida T. Real-time nanoscopy by using blinking enhanced quantum dots. Biophys. J. 2010, 99(7):L50-L52.
57. Lidke K., Rieger B., Jovin T., Heintzmann R. Superresolution by localization of quantum dots using blinking statistics. Opt. Express. 2005, 13(18):7052-7062.
58. Lagerholm B.C., Averett L., Weinreb G.E., Jacobson K., Thompson N.L. Analysis method for measuring submicroscopic distances with blinking quantum dots. Biophys. J. 2006, 91(8):3050-3060.
59. Clarke S.J., Hollmann C.A., Zhang Z., Suffern D., Bradforth S.E., Dimitrijevic N.M., Minarik W.G., Nadeau J.L. Photophysics of dopamine-modified quantum dots and effects on biological Systems. Nat. Mater. 2006, 5:409-417.
60. Khatchadourian R., Bachir A., Clarke S.J., Heyes C.D., Wiseman P.W., Nadeau J.L. Fluorescence intensity and intermittency as tools for following dopamine bioconjugate processing in living cells. J. Biomed. Biotechnol. 2007, 2007:70145.
61. J.J. Peterson , Nesbitt D.J. Modified power law behavior in quantum dot blinking: a novel role for biexcitons and auger ionization. Nano Lett. 2009, 9(1):338-345.
62. Antelman J., Ebenstein Y., Dertinger T., Michalet X., Weiss S. Suppression of quantum dot blinking in DTT-doped polymer films. J. Phys. Chem. C 2009, 113(27):11541-11545.
63. Hohng S., Ha T. Near-complete suppression of quantum dot blinking in ambient conditions. J. Am. Chem. Soc. 2004, 126(5):1324-1325.
64. Yuan C.T., Yu P., Ko H.C., Huang J., Tang J. Antibunching single-photon emission and blinking suppression of CdSe/ZnS quantum dots. ACS Nano 2009, 3(10):3051-3056.
65. 2 nanoparticles. ACS Nano 2010, 4(8):4445-4454.
66. Wang X., Ren X., Kahen K., Hahn M.A., Rajeswaran M., Maccagnano-Zacher S., Silcox J., Cragg G.E., Efros A.L., Krauss T.D. Non-blinking semiconductor nanocrystals. Nature 2009, 459:686-689.
67. Hammer N.I., Early K.T., Sill K., Odoi M.Y., Emrick T., Barnes M.D. Coverage-mediated suppression of blinking in solid state quantum dot conjugated organic composite nanostructures. J. Phys. Chem. B 2006, 110:14167-14171.
68. Fu Y., Zhang J., Lakowicz J.R. Suppressed blinking in single quantum dots (QDs) immobilized near silver island films (SIFs). Chem. Phys. Lett. 2007, 447(1-3):96-100.
69. Fomenko V., Nesbitt D.J. Solution control of radiative and nonradiative lifetimes: a novel contribution to quantum dot blinking suppression. Nano Lett. 2008, 8(1):287-293.
70. Diez M., Zimmermann B., Börsch M., König M., Schweinberger E., Steigmiller S., et al. Proton-powered subunit rotation in single membrane-bound F0F1-ATP synthase. Nat. Struct. Biol. 2004, 11:135-141.
71. Li-Shishido S., Watanabe T.M., Tada H., Higuchi H., Ohuchi N. Reduction in nonfluorescence state of quantum dots on an immunofluorescence staining. Biochem. Biophys. Res. Comm. 2006, 351:7-13.
72. Jeong S., Achermann M., Nanda J., Ivanov S., Klimov V.I., Hollingsworth J.A. Effect of the thiol-thiolate equilibrium on the photophysical properties of aqueous CdSe/ZnS nanocrystal quantum dots. J. Am. Chem. Soc. 2005, 127:10126.
73. Ammam M., Zakai U.I., Wilson G.S., Glass R.S. Anodic oxidation of m-terphenyl thio-, selenoand telluroethers: lowered oxidation potentials due to chalcogen-interaction. Pure Appl. Chem. 2010, 82(3):555-563.