Screening for cytochrome P450 expression in Pichia pastoris whole cells by P450-carbon monoxide complex determination

Biotechnology Journal

Volumn 8, Issue 1, 2013, Pages 146-152

  Gudiminchi, Rama Krishna ^a   Geier, Martina ^a   Glieder, Anton ^b   Camattari, Andrea ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

Author keywords

CO spectra; Cytochrome P450 expression; HCPR and CYP2D6 expression; High throughput; Pichia pastoris whole cells

Indexed keywords

96-WELL FORMAT; CYTOCHROME P450; EXPRESSION LEVELS; HCPR AND CYP2D6 EXPRESSION; HEMOPROTEINS; HIGH THROUGHPUT; HIGH-THROUGHPUT METHOD; P. PASTORIS; PATH LENGTH; PICHIA PASTORIS; SPECTRAL INTERFERENCE; WHOLE CELL; YEAST CELL; YEAST EXPRESSION;

CARBON MONOXIDE; CELLS; CYTOLOGY; ENZYMES; ESCHERICHIA COLI;

YEAST;

CARBON MONOXIDE; CYP52A13; CYTOCHROME; CYTOCHROME P450; CYTOCHROME P450 2D6; HEMOPROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CANDIDA; COPY NUMBER VARIATION; ESCHERICHIA COLI; FUNGAL CELL; FUNGAL STRAIN; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; WHOLE CELL; YEAST CELL;

CARBON MONOXIDE; CYTOCHROME P-450 CYP2D6; HIGH-THROUGHPUT SCREENING ASSAYS; NADPH-FERRIHEMOPROTEIN REDUCTASE; PICHIA; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE;

CANDIDA; ESCHERICHIA COLI; PICHIA PASTORIS;

EID: 84871759306     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201200185     Document Type: Article

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