Cytochromes P-450 from cassava (Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and lotaustralin. Cloning, functional expression in Pichia pastoris, and substrate specificity of the isolated recombinant enzymes

Journal of Biological Chemistry

Volumn 275, Issue 3, 2000, Pages 1966-1975

  Andersen, Mette Dahl ^a,b   Busk, Peter Kamp ^a,b   Svendsen, Ib ^c   Møller, Birger Lindberg ^a,b  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b Ctr for Molecular Plant Physiology   (Denmark)

^c CARLSBERG LABORATORY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450; GLUCOSIDE; LINAMARIN;

ARTICLE; CARBOHYDRATE SYNTHESIS; CASSAVA; CATALYSIS; ENZYME ANALYSIS; ENZYME SPECIFICITY; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PHYTOCHEMISTRY; PICHIA PASTORIS; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BLOTTING, SOUTHERN; CATALYSIS; CELLS, CULTURED; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; GLUCOSIDES; ISOLEUCINE; MANIHOT; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; NITRILES; PICHIA; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; VALINE;

MANIHOT ESCULENTA; PICHIA PASTORIS;

EID: 0034695491     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.275.3.1966     Document Type: Article

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