Production and characterization of hirudin variant-1 by SUMO fusion technology in E. coli

Molecular Biotechnology

Volumn 53, Issue 1, 2013, Pages 41-48

  Lu, Wuguang ^a   Cai, Xueting ^a   Gu, Zhenghua ^a   Huang, Yuzheng ^b   Xia, Binbin ^a   Cao, Peng ^a  

^a Jiangsu Province Institute of Traditional Chinese Medicine   (China)

^b JIANGSU INSTITUTE OF PARASITIC DISEASES   (China)

Author keywords

Escherichia coli; Hirudin; SUMO; SUMO specific protease

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; E. COLI; EXPRESSION SYSTEM; FUSION GENES; FUSION PROTEINS; FUSION TECHNOLOGY; HIRUDIN; MALDI-TOF/MS; N-TERMINAL SEQUENCING; N-TERMINALS; SMALL UBIQUITIN-RELATED MODIFIERS; SOLUBLE FRACTION; SPECIFIC ACTIVITY; SUMO; SUMO-SPECIFIC PROTEASE;

AFFINITY CHROMATOGRAPHY; CYTOLOGY; ENZYMES; ESCHERICHIA COLI; GENES; MOLECULAR WEIGHT;

PURIFICATION;

HIRUDIN VARIANT 1; HYBRID PROTEIN; SUMO PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANION EXCHANGE CHROMATOGRAPHY; ANTICOAGULATION; ARTICLE; BIOTECHNOLOGY; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN CLEAVAGE; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ANTICOAGULANTS; CHROMATOGRAPHY, AFFINITY; CYSTEINE ENDOPEPTIDASES; CYTOPLASM; ESCHERICHIA COLI; GENE EXPRESSION; HIRUDINS; MOLECULAR WEIGHT; RECOMBINANT FUSION PROTEINS; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ESCHERICHIA COLI;

EID: 84871659118     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-012-9511-1     Document Type: Article

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