Optimization of expression of an Annexin V-Hirudin chimeric protein in Escherichia coli

Microbiological Research

Volumn 159, Issue 2, 2004, Pages 147-156

  Yuan, Hui ^a   Yang, Xiang ^a   Hua, Zi Chun ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

Annexin V; Expression; Fermentation; Purification

Indexed keywords

CELLS; ESCHERICHIA COLI; OPTIMIZATION; PROTEINS;

CHIMAERIC PROTEIN; CULTURE MEDIA; EXPRESSION CAPACITY STABILITY;

MICROBIOLOGY;

GENE EXPRESSION;

ESCHERICHIA COLI;

EID: 3042596177     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2004.02.002     Document Type: Article

References (22)

1. Bevers E.M., Comfurius P., Zwaal R.F.A. Changes in membrane phospholipid distribution during platelet activation. Biochim. Biophys. Acta. 736:1983;57-66
2. Bevers E.M., Comfurius P., Zwaal R.F. Platelet procoagulant activity. physiological significance and mechanisms of exposure Blood Rev. 5:1991;146-154
3. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254
4. Fan Y., Wang J., Yang S., Yang X., Zhang L.N., Hua Z.C., Zhu D.X. Molecular dynamics simulation of docking a novel hirudin-like anti-coagulant protein to thrombin. Prog. Biochem. Biophys. 28:2001;86-89
5. Flaherty M.J., West S., Heimark R.L., Fujikawa K., Tait J.F. Placental anticoagulant protein-I. measurement in extracellular fluids and cells of the hemostatic system J. Lab. Clin. Med. 115:1990;174-181
6. Fu K., Izquierdo R., Walenga J.M., Fareed J. Comparative study on the use of anticoagulants heparin and recombinant hirudin in a rabbit traumatic anastomosis model. Thromb. Res. 78:1995;421-428
7. Funakoshi T., Heimark R.L., Hendrickson L.E., McMullen B.A., Fujikawa K. Human placental anticoagulant protein. isolation and characterization Biochemistry. 26:1987;5572-5578
8. Funakoshi T., Hendrickson L.E., McMullen B.A., Fujikawa K. Primary structure of human placental anticoagulant protein. Biochemistry. 26:1987;8087-8092
9. Hua Z.C., Dong C., Zhu D.X. Renaturation of recombinant human pro-urokinase expressed in E. coli. Biochem. Biophys. Res. Commun. 220:1996;131-136
10. Ni X.W., Gao S.W. A study of mass transfer in a pulsed baffled bioreactor. Biotechnol. Bioeng. 45:1995;165-175
11. Okabayashi K., Tsujikawa M., Morita M., Einaga K., Tanaka K., Tanabe T., Yamanouchi K., Hirama M., Tait J.F., Fujikawa K. Secretory production of recombinant urokinase-type plasminogen. Gene. 177:1996;69-76
12. Op den Kamp J.A.F. Lipid asymmetry in membranes. Annu. Rev. Biochem. 48:1979;47-71
13. Rao L.V., Tait J.F., Hoang A.D. Binding of Annexin V to a human ovarian carcinoma cell line (OC-2008). contrasting effects on cell surface factor VIIa/tissue factor activity and prothrombinase activity Thromb. Res. 67:1992;517-531
14. Rydel T.J., Ravichandran K.G., Tulinsky A. The structure of a complex of recombinant hirudin and human-thrombin. Science. 249:1990;277-280
15. Skogman G., Nilson J., Gustafsson P. The use of a partition locus to increase stability of trytophan-operon-bearing plasmids in E. coli. Gene. 23:1983;105-115
16. Stratton J.R., Dewhurst T.A., Kasina S., Reno J.M., Cerqueira M.D., Baskin D.G., Tait J.F. Circulation selective uptake of radiolabeled annexin V on acute porcine left atrial thrombi. Circulation. 92:1995;3113-3121
17. Tait J.F., Cerqueira M.D., Dewhurst T.A., Fujikawa K., Ritchie J.L., Stratton J.R. Evaluation of annexin V as a platelet-directed thrombus targeting agent. Thromb. Res. 75:1994;491-501
18. Tait J.F., Engelhardt S., Smith C., Fujikawa K. Prourokinase-annexin V chimeras. construction, expression, and characterization of recombinant proteins J. Biol. Chem. 270:1995;21594-21599
19. Tanaka K., Einaga K., Tsuchiyama H., Tait J.F., Fujikawa K. Preparation and characterization of a disulfide-linked bioconjugate of annexin V with the B-chain of urokinase. an improved fibrinolytic agent targeted to phospholipid-containing thrombi Biochemistry. 23:1996;922-929
20. Thiagarajan P., Tait J.F. Binding of annexin V/placental anticoagulant protein I to platelets. evidence for phosphatidylserine exposure in the procoagulant response of activated platelets J. Biol. Chem. 265:1990;17420-17423
21. Thiagarajan P., Tait J.F. Collagen-induced exposure of anionic phospholipid in platelets and platelet-derived microparticles. J. Biol. Chem. 266:1991;24302-24307
22. Zhou W., Chen C.C., Buckland B., Aunins J. Fed-batch culture of recombinant NS0 myeloma cells with high monoclonalantibody production. Biotechnol. Bioeng. 55:1997;783-792