High-level expression and purification of heparin-binding epidermal growth factor (HB-EGF) with SUMO fusion

Molecular Biotechnology

Volumn 44, Issue 3, 2010, Pages 198-203

  Lu, Wuguang ^a,b   Cao, Peng ^a   Lei, Huangzong ^a   Zhang, Shuangquan ^a,b  

^a Jiangsu Province Institute of Traditional Chinese Medicine   (China)

^b NANJING NORMAL UNIVERSITY   (China)

Author keywords

HB EGF; Over lap PCR; SUMO; SUMO specific protease

Indexed keywords

CELL TYPES; CELLULAR PROTEINS; CLINICAL APPLICATION; DISULFIDE BONDS; DOSE-DEPENDENT MANNER; EPIDERMAL GROWTH FACTORS; FUSION GENES; FUSION PROTEINS; GLYCOSYLATED; HEPARIN BINDING; HIGH LEVEL EXPRESSION; MITOGENIC ACTIVITY; MTT ASSAYS; SMALL UBIQUITIN-RELATED MODIFIERS;

AFFINITY CHROMATOGRAPHY; BIOACTIVITY; CHROMATOGRAPHIC ANALYSIS; ESCHERICHIA COLI; GENE EXPRESSION; PEPTIDES; POLYSACCHARIDES; PURIFICATION;

HYSTERESIS MOTORS;

GROWTH FACTOR; HEPARAN BINDING EPIDERMAL GROWTH FACTOR; HYBRID PROTEIN; NICKEL; PROTEINASE; SUMO PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; MITOGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; WESTERN BLOTTING;

3T3 CELLS; AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; COLORING AGENTS; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; MITOSIS; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; SOLUBILITY; TETRAZOLIUM SALTS; THIAZOLES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77649233734     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-009-9226-0     Document Type: Article

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