Construction, expression, and characterization of recombinant hirudin in Escherichia coli

Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

Volumn 95, Issue 1, 2001, Pages 23-30

  Bi, Q ^a   Zhang, J ^a   Huang, Y ^a   Su, H ^a   Zhou, X ^a   Zhu, S ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

Antithrombin; Expression; Purification; Recombinant hirudin HV2 K47; Secretion

Indexed keywords

ANTITHROMBIN; MUTANT PROTEIN; RECOMBINANT HIRUDIN;

ARTICLE; CHROMATOGRAPHY; DRUG ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS;

CLONING, MOLECULAR; ESCHERICHIA COLI; FIBRINOLYTIC AGENTS; HIRUDINS; MUTAGENESIS; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS;

EID: 0034836383     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1385/ABAB:95:1:23     Document Type: Article

References (9)