-
1
-
-
0021024613
-
The functional domain of hirudin, a thrombin specific inhibitor
-
Chang, J.Y. The functional domain of hirudin, a thrombin specific inhibitor. FEBS Lett. 1983, 164, 307-313.
-
(1983)
FEBS Lett.
, vol.164
, pp. 307-313
-
-
Chang, J.Y.1
-
2
-
-
0024590497
-
Purification and biochemical characterization of recombinant hirudin produced by Saccharomyces cerevisiae
-
Nadine, R.B.; Dorothee, C.; Michele, A.; Alain, V.D.; Magda, M.; Gerard, L.; Yves, L.; Stephen, W.B.; Michael, C.; Carolyn, R. Purification and biochemical characterization of recombinant hirudin produced by Saccharomyces cerevisiae. Biochemistry 1989, 28, 2941-2949.
-
(1989)
Biochemistry
, vol.28
, pp. 2941-2949
-
-
Nadine, R.B.1
Dorothee, C.2
Michele, A.3
Alain, V.D.4
Magda, M.5
Gerard, L.6
Yves, L.7
Stephen, W.B.8
Michael, C.9
Carolyn, R.10
-
3
-
-
0022543853
-
Expression, secretion and processing of hirudin in E. coli using the alkaline phosphatase signal sequence
-
Dodt, J.; Schmitz, T.; Schäfer, T.; Bergmann, C. Expression, secretion and processing of hirudin in E. coli using the alkaline phosphatase signal sequence. FEBS Lett. 1986, 202, 373-377.
-
(1986)
FEBS Lett.
, vol.202
, pp. 373-377
-
-
Dodt, J.1
Schmitz, T.2
Schäfer, T.3
Bergmann, C.4
-
4
-
-
0027619013
-
Expression, purification and characterization of multigram amounts of recombinant hybrid HV1-HV2 hirudin variant expressed in Saccharomyces cerevisiae
-
Lehman, E.D.; Joyce, J.G.; Bailey, F.J.; Henry, Z.M.; Schultz, L.D.; Christopher, T.D.; Marlene, A.J.; William, J.M. Expression, purification and characterization of multigram amounts of recombinant hybrid HV1-HV2 hirudin variant expressed in Saccharomyces cerevisiae. Protein Expres. Purif. 1993, 4, 247-255.
-
(1993)
Protein Expres. Purif.
, vol.4
, pp. 247-255
-
-
Lehman, E.D.1
Joyce, J.G.2
Bailey, F.J.3
Henry, Z.M.4
Schultz, L.D.5
Christopher, T.D.6
Marlene, A.J.7
William, J.M.8
-
5
-
-
0029591388
-
High-level secretion of hirudin by Hansenula polymorpha authentic processing of three different preprohirudins
-
Weydemann, U.; Keup, P.; Piontek, M.; Strasser, A.W.; Schweden, J.; Gellissen, G.; Janowicz, Z.A. High-level secretion of hirudin by Hansenula polymorpha authentic processing of three different preprohirudins. Appl. Microbiol. Biotechnol. 1995, 44, 377-385.
-
(1995)
Appl. Microbiol. Biotechnol.
, vol.44
, pp. 377-385
-
-
Weydemann, U.1
Keup, P.2
Piontek, M.3
Strasser, A.W.4
Schweden, J.5
Gellissen, G.6
Janowicz, Z.A.7
-
6
-
-
0030475733
-
Production and purification of recombinant hirudin expressed in the methylotrophic yeast Pichia pastoris
-
Rosenfeld, S.A.; Nadeau, D.; Tirado, J.; Hollis, G.F.; Knabb, R.M.; Jia, S. Production and purification of recombinant hirudin expressed in the methylotrophic yeast Pichia pastoris. Protein Expres. Purif. 1996, 8, 476-482.
-
(1996)
Protein Expres. Purif.
, vol.8
, pp. 476-482
-
-
Rosenfeld, S.A.1
Nadeau, D.2
Tirado, J.3
Hollis, G.F.4
Knabb, R.M.5
Jia, S.6
-
7
-
-
0036734202
-
Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate
-
Zhou, X.S.; Zhang, Y.X. Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate. Biotechnol. Lett. 2002, 24, 1449-1453.
-
(2002)
Biotechnol. Lett.
, vol.24
, pp. 1449-1453
-
-
Zhou, X.S.1
Zhang, Y.X.2
-
8
-
-
0030997654
-
Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris
-
Sreekrishna, K.; Brankamp, P.G.; Kropp, K.E.; Blankenship, D.T.; Tsay, J.T.; Smith, P.L.; Wierschke, J.D.; Subramaniam, A.; Birkenberger, L.A. Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 1997, 190, 55-62.
-
(1997)
Gene
, vol.190
, pp. 55-62
-
-
Sreekrishna, K.1
Brankamp, P.G.2
Kropp, K.E.3
Blankenship, D.T.4
Tsay, J.T.5
Smith, P.L.6
Wierschke, J.D.7
Subramaniam, A.8
Birkenberger, L.A.9
-
9
-
-
0028152841
-
C-Terminal proteolytic degradation of recombinant desulfato-hirudin and its mutants in the yeast Saccharomyces cerevisiae
-
Heim, J.; Takabayashi, K.; Meyhack, B.; Märki, W.; Pohlig, G. C-Terminal proteolytic degradation of recombinant desulfato-hirudin and its mutants in the yeast Saccharomyces cerevisiae. Eur. J. Biochem. 1994, 226, 341-353.
-
(1994)
Eur. J. Biochem.
, vol.226
, pp. 341-353
-
-
Heim, J.1
Takabayashi, K.2
Meyhack, B.3
Märki, W.4
Pohlig, G.5
-
10
-
-
0026024916
-
Liquid secondary ion mass spectrometry applied to structural confirmation of enzymically prepared C-terminal-truncated derivatives of recombinant hirudin
-
Maftouh, M.; Ponthus, C.; Tuong, A.; Picard, C. Liquid secondary ion mass spectrometry applied to structural confirmation of enzymically prepared C-terminal-truncated derivatives of recombinant hirudin. J. Chromatogr. 1991, 562, 421-434.
-
(1991)
J. Chromatogr.
, vol.562
, pp. 421-434
-
-
Maftouh, M.1
Ponthus, C.2
Tuong, A.3
Picard, C.4
-
11
-
-
0024310026
-
Isolation of recombinant hirudin by preparative high-performance liquid chromatography
-
Bischoff, R.; Clesse, D.; Whitechurch, O.; Lepage, P.; Rottsch, C. Isolation of recombinant hirudin by preparative high-performance liquid chromatography. J. Chromatogr. 1989, 476, 245-255.
-
(1989)
J. Chromatogr.
, vol.476
, pp. 245-255
-
-
Bischoff, R.1
Clesse, D.2
Whitechurch, O.3
Lepage, P.4
Rottsch, C.5
-
12
-
-
0025148336
-
Isolation and purification of novel hirudin from the leech Hirudinaria manillensis by high-performance liquid chromatography
-
Steiner, V.; Knecht, R.; Gruetter, M. Isolation and purification of novel hirudin from the leech Hirudinaria manillensis by high-performance liquid chromatography. J. Chromatogr. 1990, 530, 273-282.
-
(1990)
J. Chromatogr.
, vol.530
, pp. 273-282
-
-
Steiner, V.1
Knecht, R.2
Gruetter, M.3
-
13
-
-
0032933572
-
Downstream processing of recombinant hirudin produced in Saccharomyces cerevisiae
-
Chung, B.H.; Kim, W.K.; Rao, K. Downstream processing of recombinant hirudin produced in Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 1999, 9, 179-183.
-
(1999)
J. Microbiol. Biotechnol.
, vol.9
, pp. 179-183
-
-
Chung, B.H.1
Kim, W.K.2
Rao, K.3
-
14
-
-
0027170369
-
Nonurea sodium dodecyl sulfate-polyacrylamide gel electrophoresis with high-molarity buffers for the separation of proteins and peptides
-
Okajima, T.; Tanabe, T.; Yasuda, T. Nonurea sodium dodecyl sulfate-polyacrylamide gel electrophoresis with high-molarity buffers for the separation of proteins and peptides. Anal. Biochem. 1993, 211, 293-300.
-
(1993)
Anal. Biochem.
, vol.211
, pp. 293-300
-
-
Okajima, T.1
Tanabe, T.2
Yasuda, T.3
-
15
-
-
0035525447
-
Decolorization and isolation of rrecombinant hirudin expressed in the methylotrophic yeast Pichia pastoris
-
Zhou, W.-B.; Zhou, X.-S.; Zhang, Y.-X. Decolorization and isolation of rrecombinant hirudin expressed in the methylotrophic yeast Pichia pastoris. Chin. J. Biotech. 2001, 17, 683-687.
-
(2001)
Chin. J. Biotech.
, vol.17
, pp. 683-687
-
-
Zhou, W.-B.1
Zhou, X.-S.2
Zhang, Y.-X.3
-
17
-
-
0025860279
-
A fast photometric assay for the determination of hirudin
-
Spannagl, M.; Bichler, H.; Lill, H.; Schramm, W. A fast photometric assay for the determination of hirudin. Haemostasis 1991, 21 (suppl.1), 36-40.
-
(1991)
Haemostasis
, vol.21
, Issue.1 SUPPL.
, pp. 36-40
-
-
Spannagl, M.1
Bichler, H.2
Lill, H.3
Schramm, W.4
-
18
-
-
0025859703
-
Stability of hirudin, a thrombin specific inhibitor
-
Chang, J.Y. Stability of hirudin, a thrombin specific inhibitor. J. Biol. Chem. 1991, 266, 10839-10843.
-
(1991)
J. Biol. Chem.
, vol.266
, pp. 10839-10843
-
-
Chang, J.Y.1
-
19
-
-
0025947075
-
Characterization, stability and refolding of recombinant hirudin
-
Otto, A.; Seckler, R. Characterization, stability and refolding of recombinant hirudin. Eur. J. Biochem. 1991, 202, 67-73.
-
(1991)
Eur. J. Biochem.
, vol.202
, pp. 67-73
-
-
Otto, A.1
Seckler, R.2
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