메뉴 건너뛰기




Volumn 27, Issue 2, 2010, Pages 108-117

V-gene amplification revisited - An optimised procedure for amplification of rearranged human antibody genes of different isotypes

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY GENES; BIOINFORMATIC ANALYSIS; CDNA SYNTHESIS; COMMERCIAL ENZYMES; GENE AMPLIFICATION; GENE-SPECIFIC PRIMERS; HUMAN ANTIBODIES; KEY PARAMETERS; PCR EFFICIENCY; PERIPHERAL BLOOD MONONUCLEAR CELLS; PHAGE DISPLAY; REVERSE TRANSCRIPTASES;

EID: 77952243807     PISSN: 18716784     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.nbt.2010.01.001     Document Type: Article
Times cited : (28)

References (48)
  • 1
    • 27644489367 scopus 로고    scopus 로고
    • Immunoglobulin gene diversification
    • Maizels N. Immunoglobulin gene diversification. Annu. Rev. Genet. 39 (2005) 23-46
    • (2005) Annu. Rev. Genet. , vol.39 , pp. 23-46
    • Maizels, N.1
  • 2
    • 13444301423 scopus 로고    scopus 로고
    • VBASE2, an integrative V-gene database
    • Retter I., et al. VBASE2, an integrative V-gene database. Nucleic Acids Res. 33 (2005) D671-D674
    • (2005) Nucleic Acids Res. , vol.33
    • Retter, I.1
  • 3
    • 77952242139 scopus 로고    scopus 로고
    • http://www.vbase2.org/
  • 4
    • 25644448735 scopus 로고    scopus 로고
    • Efficient construction of a highly useful phage-displayed human antibody repertoire
    • Rojas G., et al. Efficient construction of a highly useful phage-displayed human antibody repertoire. Biochem. Biophys. Res. Commun. 336 (2005) 1207-1213
    • (2005) Biochem. Biophys. Res. Commun. , vol.336 , pp. 1207-1213
    • Rojas, G.1
  • 5
    • 34249699643 scopus 로고    scopus 로고
    • B cells in autoimmune diseases: insights from analyses of immunoglobulin variable (Ig V) gene usage
    • Foreman A.L., et al. B cells in autoimmune diseases: insights from analyses of immunoglobulin variable (Ig V) gene usage. Autoimmun Rev. 6 (2007) 387-401
    • (2007) Autoimmun Rev. , vol.6 , pp. 387-401
    • Foreman, A.L.1
  • 6
    • 0029549091 scopus 로고
    • The expressed heavy chain V-gene repertoire of circulating B cells in normal adults
    • Stollar B.D. The expressed heavy chain V-gene repertoire of circulating B cells in normal adults. Ann. N Y Acad. Sci. 764 (1995) 265-274
    • (1995) Ann. N Y Acad. Sci. , vol.764 , pp. 265-274
    • Stollar, B.D.1
  • 8
    • 0028955645 scopus 로고
    • Amino acid sequence based PCR primers for amplification of rearranged human heavy and light chain immunoglobulin variable region genes
    • Welschof M., et al. Amino acid sequence based PCR primers for amplification of rearranged human heavy and light chain immunoglobulin variable region genes. J. Immunol. Methods 179 (1995) 203-214
    • (1995) J. Immunol. Methods , vol.179 , pp. 203-214
    • Welschof, M.1
  • 9
    • 0001890686 scopus 로고    scopus 로고
    • Construction and screening of antibody display libraries
    • Kay B.K., Winter J., and McCafferty J. (Eds), Academic Press
    • McCafferty J., and Johnson K.S. Construction and screening of antibody display libraries. In: Kay B.K., Winter J., and McCafferty J. (Eds). Phage Display of Peptides and Proteins (1996), Academic Press 79-111
    • (1996) Phage Display of Peptides and Proteins , pp. 79-111
    • McCafferty, J.1    Johnson, K.S.2
  • 10
    • 0031933472 scopus 로고    scopus 로고
    • A definitive set of oligonucleotide primers for amplifying human V regions
    • Sblattero D., and Bradbury A. A definitive set of oligonucleotide primers for amplifying human V regions. Immunotechnology 3 (1998) 271-278
    • (1998) Immunotechnology , vol.3 , pp. 271-278
    • Sblattero, D.1    Bradbury, A.2
  • 11
    • 0028059591 scopus 로고
    • Isolation of IgG antibody Fv-DNA from various mouse and rat by bridoma cell lines using the polymerase chain reaction with a simple set of primers
    • Dübel S., et al. Isolation of IgG antibody Fv-DNA from various mouse and rat by bridoma cell lines using the polymerase chain reaction with a simple set of primers. J. Immunol. Methods 175 (1994) 89-95
    • (1994) J. Immunol. Methods , vol.175 , pp. 89-95
    • Dübel, S.1
  • 12
    • 0033958471 scopus 로고    scopus 로고
    • Universal PCR amplification of mouse immunoglobulin gene variable regions: the design of degenerate primers and an assessment of the effect of DNA polymerase 3′ to 5′ exonuclease activity
    • Wang Z., et al. Universal PCR amplification of mouse immunoglobulin gene variable regions: the design of degenerate primers and an assessment of the effect of DNA polymerase 3′ to 5′ exonuclease activity. J. Immunol. Methods 233 (2000) 167-177
    • (2000) J. Immunol. Methods , vol.233 , pp. 167-177
    • Wang, Z.1
  • 13
    • 40649104101 scopus 로고    scopus 로고
    • Design and testing of PCR primers for the construction of scFv libraries representing the immunoglobulin repertoire of rats
    • Sepulveda J., and Shoemaker C.B. Design and testing of PCR primers for the construction of scFv libraries representing the immunoglobulin repertoire of rats. J. Immunol. Methods 332 (2008) 92-102
    • (2008) J. Immunol. Methods , vol.332 , pp. 92-102
    • Sepulveda, J.1    Shoemaker, C.B.2
  • 14
    • 0028825910 scopus 로고
    • Selection of specific phage-display antibodies using libraries derived from chicken immunoglobulin genes
    • Davies E.L., et al. Selection of specific phage-display antibodies using libraries derived from chicken immunoglobulin genes. J. Immunol. Methods 186 (1995) 125-135
    • (1995) J. Immunol. Methods , vol.186 , pp. 125-135
    • Davies, E.L.1
  • 15
    • 84870893908 scopus 로고    scopus 로고
    • The polymerase chain reaction
    • Powledge T.M. The polymerase chain reaction. Adv. Physiol. Educ. 28 (2004) 44-50
    • (2004) Adv. Physiol. Educ. , vol.28 , pp. 44-50
    • Powledge, T.M.1
  • 16
    • 0141625782 scopus 로고    scopus 로고
    • Amplification efficiency of thermostable DNA polymerases
    • Arezi B., et al. Amplification efficiency of thermostable DNA polymerases. Anal. Biochem. 321 (2003) 226-235
    • (2003) Anal. Biochem. , vol.321 , pp. 226-235
    • Arezi, B.1
  • 17
    • 77952242758 scopus 로고    scopus 로고
    • http://vbase.mrc-cpe.cam.ac.uk/
  • 18
    • 9144233545 scopus 로고    scopus 로고
    • The EMBL Nucleotide Sequence Database
    • Kulikova T., et al. The EMBL Nucleotide Sequence Database. Nucleic Acids Res. 32 (2004) D27-D30
    • (2004) Nucleic Acids Res. , vol.32
    • Kulikova, T.1
  • 19
    • 2442706422 scopus 로고    scopus 로고
    • An overview of ensembl
    • Birney E., et al. An overview of ensembl. Genome Res. 14 (2004) 925-928
    • (2004) Genome Res. , vol.14 , pp. 925-928
    • Birney, E.1
  • 20
    • 0035171080 scopus 로고    scopus 로고
    • Kabat database and its applications: future directions
    • Johnson G., and Wu T.T. Kabat database and its applications: future directions. Nucleic Acids Res. 29 (2001) 205-206
    • (2001) Nucleic Acids Res. , vol.29 , pp. 205-206
    • Johnson, G.1    Wu, T.T.2
  • 21
    • 0030814645 scopus 로고    scopus 로고
    • IMGT, the international ImMunoGeneTics database
    • Giudicelli V., et al. IMGT, the international ImMunoGeneTics database. Nucleic Acids Res. 25 (1997) 206-211
    • (1997) Nucleic Acids Res. , vol.25 , pp. 206-211
    • Giudicelli, V.1
  • 22
    • 33644874839 scopus 로고    scopus 로고
    • IMGT/LIGM-DB, the IMGT comprehensive database of immunoglobulin and T cell receptor nucleotide sequences
    • Giudicelli V., et al. IMGT/LIGM-DB, the IMGT comprehensive database of immunoglobulin and T cell receptor nucleotide sequences. Nucleic Acids Res. 34 (2006) D781-D784
    • (2006) Nucleic Acids Res. , vol.34
    • Giudicelli, V.1
  • 23
    • 34249790004 scopus 로고    scopus 로고
    • Molecular mechanisms of antibody somatic hypermutation
    • Di Noia J.M., and Neuberger M.S. Molecular mechanisms of antibody somatic hypermutation. Ann. Rev. Biochem. 76 (2007) 1-22
    • (2007) Ann. Rev. Biochem. , vol.76 , pp. 1-22
    • Di Noia, J.M.1    Neuberger, M.S.2
  • 24
    • 34548743476 scopus 로고    scopus 로고
    • Gene conversion: mechanisms, evolution and human disease
    • Chen J.M., et al. Gene conversion: mechanisms, evolution and human disease. Nat. Rev. Genet. 8 (2007) 762-775
    • (2007) Nat. Rev. Genet. , vol.8 , pp. 762-775
    • Chen, J.M.1
  • 25
    • 0033948368 scopus 로고    scopus 로고
    • Repertoire selection by pre-B-cell receptors and B-cell receptors, and genetic control of B-cell development from immature to mature B cells
    • Melchers F., et al. Repertoire selection by pre-B-cell receptors and B-cell receptors, and genetic control of B-cell development from immature to mature B cells. Immunol. Rev. 175 (2000) 33-46
    • (2000) Immunol. Rev. , vol.175 , pp. 33-46
    • Melchers, F.1
  • 26
    • 1542328972 scopus 로고    scopus 로고
    • Properties of the reverse transcription reaction in mRNA quantification
    • Stahlberg A., et al. Properties of the reverse transcription reaction in mRNA quantification. Clin. Chem. 50 (2004) 509-515
    • (2004) Clin. Chem. , vol.50 , pp. 509-515
    • Stahlberg, A.1
  • 27
    • 0032979157 scopus 로고    scopus 로고
    • Comparison of specific and random priming in the reverse transcriptase polymerase chain reaction for genotyping group A rotaviruses
    • Iturriza-Gomara M., et al. Comparison of specific and random priming in the reverse transcriptase polymerase chain reaction for genotyping group A rotaviruses. J Virol. Methods 78 (1999) 93-103
    • (1999) J Virol. Methods , vol.78 , pp. 93-103
    • Iturriza-Gomara, M.1
  • 29
    • 37549052481 scopus 로고    scopus 로고
    • Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR
    • Levesque-Sergerie J.P., et al. Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR. BMC Mol. Biol. 8 (2007) 93
    • (2007) BMC Mol. Biol. , vol.8 , pp. 93
    • Levesque-Sergerie, J.P.1
  • 30
    • 29144495882 scopus 로고    scopus 로고
    • PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
    • Suslov O., and Steindler D.A. PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency. Nucleic Acids Res. 33 (2005) e181
    • (2005) Nucleic Acids Res. , vol.33
    • Suslov, O.1    Steindler, D.A.2
  • 31
    • 0031889693 scopus 로고    scopus 로고
    • Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR
    • Chandler D.P., et al. Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR. Appl. Environ. Microbiol. 64 (1998) 669-677
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 669-677
    • Chandler, D.P.1
  • 32
    • 0000374167 scopus 로고    scopus 로고
    • Capacity of nine thermostable DNA polymerases To mediate DNA amplification in the presence of PCR-inhibiting samples
    • Abu Al-Soud W., and Radstrom P. Capacity of nine thermostable DNA polymerases To mediate DNA amplification in the presence of PCR-inhibiting samples. Appl. Environ. Microbiol. 64 (1998) 3748-3753
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 3748-3753
    • Abu Al-Soud, W.1    Radstrom, P.2
  • 33
    • 11844276633 scopus 로고    scopus 로고
    • Experimental procedures comparing the activity of different Taq polymerases
    • Spitaleri S., et al. Experimental procedures comparing the activity of different Taq polymerases. Forensic Sci. Int. 146 (2004) S167-S169
    • (2004) Forensic Sci. Int. , vol.146
    • Spitaleri, S.1
  • 34
    • 0022555837 scopus 로고
    • Single-stranded DNA binding proteins required for DNA replication
    • Chase J.W., and Williams K.R. Single-stranded DNA binding proteins required for DNA replication. Annu. Rev. Biochem. 55 (1986) 103-136
    • (1986) Annu. Rev. Biochem. , vol.55 , pp. 103-136
    • Chase, J.W.1    Williams, K.R.2
  • 35
    • 34147224324 scopus 로고
    • The single-stranded DNA-binding protein of Escherichia coli
    • Meyer R.R., and Laine P.S. The single-stranded DNA-binding protein of Escherichia coli. Microbiol. Rev. 54 (1990) 342-380
    • (1990) Microbiol. Rev. , vol.54 , pp. 342-380
    • Meyer, R.R.1    Laine, P.S.2
  • 36
    • 0344909264 scopus 로고
    • Single-strand binding protein enhances fidelity of DNA synthesis in vitro
    • Kunkel T.A., et al. Single-strand binding protein enhances fidelity of DNA synthesis in vitro. Proc. Natl. Acad. Sci. U.S.A. 76 (1979) 6331-6335
    • (1979) Proc. Natl. Acad. Sci. U.S.A. , vol.76 , pp. 6331-6335
    • Kunkel, T.A.1
  • 37
    • 0026662508 scopus 로고
    • Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB
    • Chou Q. Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB. Nucleic Acids Res. 20 (1992) 4371
    • (1992) Nucleic Acids Res. , vol.20 , pp. 4371
    • Chou, Q.1
  • 38
    • 3242890386 scopus 로고    scopus 로고
    • Many levels of control of V-gene rearrangement frequency
    • Feeney A.J., et al. Many levels of control of V-gene rearrangement frequency. Immunol. Rev. 200 (2004) 44-56
    • (2004) Immunol. Rev. , vol.200 , pp. 44-56
    • Feeney, A.J.1
  • 39
    • 33745323376 scopus 로고    scopus 로고
    • Trends in antibody sequence changes during the somatic hypermutation process
    • Clark L.A., et al. Trends in antibody sequence changes during the somatic hypermutation process. J. Immunol. 177 (2006) 333-340
    • (2006) J. Immunol. , vol.177 , pp. 333-340
    • Clark, L.A.1
  • 40
    • 13544277164 scopus 로고    scopus 로고
    • Variable-region-identical antibodies differing in isotype demonstrate differences in fine specificity and idiotype
    • Torres M., et al. Variable-region-identical antibodies differing in isotype demonstrate differences in fine specificity and idiotype. J. Immunol. 174 (2005) 2132-2142
    • (2005) J. Immunol. , vol.174 , pp. 2132-2142
    • Torres, M.1
  • 41
    • 0242273359 scopus 로고    scopus 로고
    • The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus
    • Fraser N.L., et al. The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus. Arthritis Res. Ther. 5 (2003) R114-R121
    • (2003) Arthritis Res. Ther. , vol.5
    • Fraser, N.L.1
  • 42
    • 0342300209 scopus 로고    scopus 로고
    • Use of immunoglobulin variable-region genes by normal subjects and patients with systemic lupus erythematosus
    • Hansen A., et al. Use of immunoglobulin variable-region genes by normal subjects and patients with systemic lupus erythematosus. Int. Arch. Allergy Immunol. 123 (2000) 36-45
    • (2000) Int. Arch. Allergy Immunol. , vol.123 , pp. 36-45
    • Hansen, A.1
  • 43
    • 34247566952 scopus 로고    scopus 로고
    • Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren's syndrome
    • Jacobi A.M., et al. Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren's syndrome. Arthritis. Res. 4 (2002) R4
    • (2002) Arthritis. Res. , vol.4
    • Jacobi, A.M.1
  • 44
    • 17644399099 scopus 로고    scopus 로고
    • Molecular basis of immunoglobulin variable region gene usage in systemic autoimmunity
    • Dörner T., and Lipsky P.E. Molecular basis of immunoglobulin variable region gene usage in systemic autoimmunity. Clin. Exp. Med. 4 (2005) 159-169
    • (2005) Clin. Exp. Med. , vol.4 , pp. 159-169
    • Dörner, T.1    Lipsky, P.E.2
  • 45
    • 2942614692 scopus 로고    scopus 로고
    • Utilization of Ig heavy chain variable, diversity, and joining gene segments in children with B-lineage acute lymphoblastic leukemia: implications for the mechanisms of VDJ recombination and for pathogenesis
    • Li A., et al. Utilization of Ig heavy chain variable, diversity, and joining gene segments in children with B-lineage acute lymphoblastic leukemia: implications for the mechanisms of VDJ recombination and for pathogenesis. Blood 103 (2004) 4602-4609
    • (2004) Blood , vol.103 , pp. 4602-4609
    • Li, A.1
  • 46
    • 85017685667 scopus 로고    scopus 로고
    • Selection of recombinant antibodies from antibody gene libraries
    • Hust M., et al. Selection of recombinant antibodies from antibody gene libraries. Methods Mol. Biol. 408 (2007) 243-255
    • (2007) Methods Mol. Biol. , vol.408 , pp. 243-255
    • Hust, M.1
  • 47
    • 0029085136 scopus 로고
    • Human antibody libraries in Escherichia coli
    • Little M., et al. Human antibody libraries in Escherichia coli. J. Biotechnol. 41 (1995) 187-195
    • (1995) J. Biotechnol. , vol.41 , pp. 187-195
    • Little, M.1
  • 48
    • 0027008594 scopus 로고
    • Building antibodies from their genes
    • Hoogenboom H.R., et al. Building antibodies from their genes. Immunol. Rev. 130 (1992) 41-68
    • (1992) Immunol. Rev. , vol.130 , pp. 41-68
    • Hoogenboom, H.R.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.