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Volumn 92, Issue 8, 2010, Pages 1081-1084

A convenient and robust method for construction of combinatorial and random mutant libraries

Author keywords

DNA shuffling; Error prone PCR; Ligase independent cloning; Mutant library

Indexed keywords

DNA; LIGASE;

EID: 77954658425     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2010.03.016     Document Type: Article
Times cited : (8)

References (10)
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  • 2
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    • Rapid evolution of a protein in vitro by DNA shuffling
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  • 3
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    • DNA shuffling of a family of genes from diverse species accelerates directed evolution
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    • Crameri, A.1    Raillard, S.A.2    Bermudez, E.3    Stemmer, W.P.4
  • 4
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    • Novel family shuffling methods for the in vitro evolution of enzymes
    • Kikuchi M., Ohnishi K., Harayama S. Novel family shuffling methods for the in vitro evolution of enzymes. Gene 1999, 236:159-167.
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    • Kikuchi, M.1    Ohnishi, K.2    Harayama, S.3
  • 5
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    • Random DNA fragmentation with endonuclease V: application to DNA shuffling
    • Miyazaki K. Random DNA fragmentation with endonuclease V: application to DNA shuffling. Nucleic Acids Res. 2002, 30:e139.
    • (2002) Nucleic Acids Res. , vol.30
    • Miyazaki, K.1
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    • An effective family shuffling method using single-stranded DNA
    • Kikuci M., Ohnishi K., Harayama S. An effective family shuffling method using single-stranded DNA. Gene 2000, 243:133-137.
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    • Kikuci, M.1    Ohnishi, K.2    Harayama, S.3
  • 7
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    • Semi-synthetic DNA shuffling of aveC leads to improved industrial scale production of doramectin by Streptomyces avermitilis
    • Stutzman-Engwall K., Conlon S., Fedechko R., et al. Semi-synthetic DNA shuffling of aveC leads to improved industrial scale production of doramectin by Streptomyces avermitilis. Metab. Eng. 2005, 7:27-37.
    • (2005) Metab. Eng. , vol.7 , pp. 27-37
    • Stutzman-Engwall, K.1    Conlon, S.2    Fedechko, R.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.