Antibody Fragments Directed against Different Portions of the Human Neural Cell Adhesion Molecule L1 Act as Inhibitors or Activators of L1 Function

PLoS ONE

Volumn 7, Issue 12, 2012, Pages

  Wang, Yan ^a   Loers, Gabriele ^b   Pan, Hong Chao ^a   Gouveia, Ricardo ^c   Zhao, Wei Jiang ^a   Shen, Yan Qin ^a   Kleene, Ralf ^b   Costa, Julia ^c   Schachner, Melitta ^a,d  

^a SHANTOU UNIVERSITY MEDICAL COLLEGE   (China)

^b UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^c INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^d RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FIBRONECTIN; FIBRONECTIN 3; HYDROGEN PEROXIDE; IMMUNOGLOBULIN; MITOGEN ACTIVATED PROTEIN KINASE; NERVE CELL ADHESION MOLECULE; PROTEIN BAX; PROTEIN BCL 2; PROTEIN L1; PROTEIN TYROSINE KINASE; RECOMBINANT ANTIBODY; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CELL MIGRATION; CELL PROLIFERATION; CELL SURFACE; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PHOSPHORYLATION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; NERVE FIBER GROWTH; NEUROBLASTOMA CELL; NONHUMAN; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; WESTERN BLOTTING;

CELL LINE; CELL MOVEMENT; CELL PROLIFERATION; HUMANS; HYDROGEN PEROXIDE; NEURAL CELL ADHESION MOLECULE L1; NEURITES; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES; SRC-FAMILY KINASES;

EID: 84871326648     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0052404     Document Type: Article

References (55)

1. Rathjen F, Schachner M, (1984) Immunocytological and biochemical characterization of a new neuronal cell surface component (L1 antigen) which is involved in cell adhesion. EMBO J 3: 1-10.
2. Faissner A, Kruse J, Nieke J, Schachner M, (1984) Expression of neural cell adhesion molecule L1 during development, in neurological mutants and in the peripheral nervous system. Brain Res 17: 69-82.
3. Kruse J, Mailhammer R, Wernecke H, Faissner A, Sommer I, et al. (1984) Neural cell adhesion molecules and myelin-associated glycoprotein share a common carbohydrate moiety recognized by monoclonal antibodies L2 and HNK-1. Nature 311: 153-155.
4. Lindner J, Rathjen FG, Schachner M, (1983) L1 mono- and polyclonal antibodies modify cell migration in early postnatal mouse cerebellum. Nature 305: 427-430.
5. Fischer G, Kunemund V, Schachner M, (1986) Neurite outgrowth patterns in cerebellar microexplant cultures are affected by antibodies to the cell surface glycoprotein L1. J Neurosci 6: 605-612.
6. Martini R, Schachner M, (1986) Immunoelectron microscopic localization of neural cell adhesion molecules (L1, N-CAM, and MAG) and their shared carbohydrate epitope and myelin basic protein in developing sciatic nerve. J Cell Biol 103: 2439-2448.
7. Seilheimer B, Schachner M, (1988) Neural cell adhesion molecule expression is regulated by Schwann cell-neuron interactions in culture. J Cell Biol 107: 341-351.
8. Moos M, Tacke R, Scherer H, Teplow D, Fruh K, et al. (1988) Neural adhesion molecule L1 as a member of the immunoglobulin superfamily with binding domains similar to fibronectin. Nature 334: 701-703.
9. Appel F, Holm J, Conscience JF, Schachner M, (1993) Several extracellular domains of the neural cell adhesion molecule L1 are involved in neurite outgrowth and cell body adhesion. J Neurosci 13: 4764-4775.
10. Wood PM, Schachner M, Bunge RP, (1990) Inhibition of Schwann cell myelination in vitro by antibody to the L1 adhesion molecule. J Neurosci 10: 3635-3645.
11. Lüthl A, Laurent JP, Figurov A, Muller D, Schachner M, (1994) Hippocampal long-term potentiation and neural cell adhesion molecules L1 and NCAM. Nature 372: 777-779.
12. Fransen E, Vits L, Van Camp G, Willems PJ, (1996) The clinical spectrum of mutations in L1, a neuronal cell adhesion molecule. Am J Med Genet 64: 73-77.
13. Timor-Tritsch IE, Monteagudo A, Haratz-Rubinstein N, Levine RU, (1996) Transvaginal sonographic detection of adducted thumbs, hydrocephalus, and agenesis of the corpus callosum at 22 postmenstrual weeks: the masa spectrum or L1 spectrum. A case report and review of the literature. Prenat Diagn 16: 543-548.
14. Poltorak M, Khoja I, Hemperly JJ, Williams JR, el-Mallakh R, et al. (1995) Disturbances in cell recognition molecules (N-CAM and L1 antigen) in the CSF of patients with schizophrenia. Exp Neurol 131: 266-272.
15. Kurumaji A, Nomoto H, Okano T, Toru M, (2001) An association study between polymorphism of L1CAM gene and schizophrenia in a Japanese sample. Am J Med Genet 105: 99-104.
16. Schafer MK, Altevogt P, (2010) L1CAM malfunction in the nervous system and human carcinomas. Cell Mol Life Sci 67: 2425-2437.
17. Christofori G, (2003) Changing neighbours, changing behaviour: cell adhesion molecule-mediated signalling during tumour progression. EMBO J 22: 2318-2323.
18. Siesser PF, Maness PF, (2009) L1 cell adhesion molecules as regulators of tumor cell invasiveness. Cell Adh Migr 3: 275-277.
19. Gouveia RM, Gomes CM, Sousa M, Alves PM, Costa J, (2008) Kinetic analysis of L1 homophilic interaction: role of the first four immunoglobulin domains and implications on binding mechanism. J Biol Chem 283: 28038-28047.
20. Eteshola E, (2010) Isolation of scFv fragments specific for monokine induced by interferon-gamma (MIG) using phage display. J Immunol Methods 358: 104-110.
21. Hoefnagel CA, Rutgers M, Buitenhuis CK, Smets LA, de Kraker J, et al. (2011) A comparison of targeting of neuroblastoma with mlBG and anti L1-CAM antibody mAb chCE7: therapeutic efficacy in a neuroblastoma xenograft model and imaging of neuroblastoma patients. Eur J Nucl Med 38: 359-368.
22. Gouveia R, Kandzia S, Conradt HS, Costa J, (2010) Production and N-glycosylation of recombinant human cell adhesion molecule L1 from insect cells using the stable expression system. Effect of dimethyl sulfoxide. J Biotechnol 145: 130-138.
23. Gouveia R, Schaffer L, Papp S, Grammel N, Kandzia S, et al. (2012) Expression of glycogenes in differentiating human NT2N neurons. Downregulation of fucosyltransferase 9 leads to decreased Lewis(x) levels and impaired neurite outgrowth. Biochim Biophys Acta 1820: 2007-2019.
24. Dong L, Chen S, Schachner M, (2003) Single chain Fv antibodies against neural cell adhesion molecule L1 trigger L1 functions in cultured neurons. Mol Cell Neurosci 22: 234-247.
25. Appel F, Holm J, Conscience JF, von Bohlen und Halbach F, Faissner A, et al. (1995) Identification of the border between fibronectin type III homologous repeats 2 and 3 of the neural cell adhesion molecule L1 as a neurite outgrowth promoting and signal transducing domain. J Neurobiol 28: 297-312.
26. Yip PM, Zhao X, Montgomery AM, Siu CH, (1998) The Arg-Gly-Asp motif in the cell adhesion molecule L1 promotes neurite outgrowth via interaction with the alphavbeta3 integrin. Mol Biol Cell 9: 277-290.
27. Maness PF, Schachner M, (2007) Neural recognition molecules of the immunoglobulin superfamily: signaling transducers of axon guidance and neuronal migration. Nat Neurosci 10: 19-26.
28. Blaess S, Kammerer RA, Hall H, (1998) Structural analysis of the sixth immunoglobulin-like domain of mouse neural cell adhesion molecule L1 and its interactions with alpha(v)beta3, alpha(IIb)beta3, and alpha5beta1 integrins. J Neurochem 71: 2615-2625.
29. Thelen K, Kedar V, Panicker AK, Schmid RS, Midkiff BR, et al. (2002) The neural cell adhesion molecule L1 potentiates integrin-dependent cell migration to extracellular matrix proteins. J Neurosci 22: 4918-4931.
30. Silletti S, Mei F, Sheppard D, Montgomery AM, (2000) Plasmin-sensitive dibasic sequences in the third fibronectin-like domain of L1-cell adhesion molecule (CAM) facilitate homomultimerization and concomitant integrin recruitment. J Cell Biol 147: 1485-1502.
31. Loers G, Chen S, Grumet M, Schachner M, (2005) Signal transduction pathways implicated in neural recognition molecule L1 triggered neuroprotection and neuritogenesis. J Neurochem 92: 1463-1476.
32. Kenwrick S, Watkins A, De Angelis E, (2000) Neural cell recognition molecule L1: relating biological complexity to human disease mutations. Hum Mol Genet 9: 879-886.
33. Pavlou O, Theodorakis K, Falk J, Kutsche M, Schachner M, et al. (2002) Analysis of interactions of the adhesion molecule TAG-1 and its domains with other immunoglobulin superfamily members. Mol Cell Neurosci 20: 367-381.
34. Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, et al. (2009) Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth. J Neurosci 29: 7624-7636.
35. Kadmon G, Kowitz A, Altevogt P, Schachner M, (1990) Functional cooperation between the neural adhesion molecules L1 and N-CAM is carbohydrate dependent. J Cell Biol 110: 209-218.
36. Kadmon G, Kowitz A, Altevogt P, Schachner M, (1990) The neural cell adhesion molecule N-CAM enhances L1-dependet cell-cell interactions. J Cell Biol 110: 193-208.
37. Buchstaller A, Kunz S, Berger P, Kunz B, Ziegler U, et al. (1996) Cell adhesion molecules NgCAM and axonin-1 form heterodimers in the neuronal membrane and cooperate in neurite outgrowth promotion. J Cell Biol 135: 1593-1607.
38. Kamiguchi H, Lemmon V, (1997) Neural cell adhesion molecule L1: signaling pathways and growth cone motility. J Neurosci Res 49: 1-8.
39. Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, et al. (2009) Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth. J Neurosci 29: 6677-6690.
40. Islam R, Kristiansen LV, Romani S, Garcia-Alonso L, Hortsch M, (2004) Activation of EGF receptor kinase by L1-mediated homophilic cell interactions. Mol Biol Cell 15: 2003-2012.
41. Hall H, Bozic D, Fauser C, Engel J, (2000) Trimerization of cell adhesion molecule L1 mimics clustered L1 expression on the cell surface: influence on L1-ligand interactions and on promotion of neurite outgrowth. J Neurochem 75: 336-346.
42. He Y, Jensen GJ, Bjorkman PJ, (2009) Cryo-electron tomography of homophilic adhesion mediated by the neural cell adhesion molecule L1. Structure 17: 460-471.
43. Montgomery AM, Becker JC, Siu CH, Lemmon VP, Cheresh DA, et al. (1996) Human neural cell adhesion molecule L1 and rat homologue NILE are ligands for integrin alpha v beta 3. J Cell Biol 132: 475-485.
44. Itoh K, Fushiki S, Kamiguchi H, Arnold B, Altevogt P, et al. (2005) Disrupted Schwann cell-axon interactions in peripheral nerves of mice with altered L1-integrin interactions. Mol Cell Neurosci 30: 624-629.
45. Haspel J, Friedlander DR, Ivgy-May N, Chickramane S, Roonprapunt C, et al. (2000) Critical and optimal Ig domains for promotion of neurite outgrowth by L1/Ng-CAM. J Neurobiol 42: 287-302.
46. Hall H, Djonov V, Ehrbar M, Hoechli M, Hubbell JA, (2004) Heterophilic interactions between cell adhesion molecule L1 and alphavbeta3-integrin induce HUVEC process extension in vitro and angiogenesis in vivo. Angiogenesis 7: 213-223.
47. Drescher B, Spiess E, Schachner M, Probstmeier R, (1996) Structural analysis of the murine cell adhesion molecule L1 by electron microscopy and computer-assisted modelling. Eur J Neurosci 8: 2467-2478.
48. Miyahara R, Tanaka F, Nakagawa T, Matsuoka K, Isii K, et al. (2001) Expression of neural cell adhesion molecules (polysialylated form of neural cell adhesion molecule and L1-cell adhesion molecule) on resected small cell lung cancer specimens: in relation to proliferation state. J Surg Oncol 77: 49-54.
49. Wolterink S, Moldenhauer G, Fogel M, Kiefel H, Pfeifer M, et al. (2010) Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer Res 70: 2504-2515.
50. Wei CH, Lee ES, Jeon JY, Heo YS, Kim SJ, et al. (2011) Structural mechanism of the antigen recognition by the L1 cell adhesion molecule antibody A10-A3. FEBS Lett 585: 153-158.
51. Min JK, Kim JM, Li S, Lee JW, Yoon H, et al. (2010) L1 cell adhesion molecule is a novel therapeutic target in intrahepatic cholangiocarcinoma. Clin Cancer Res 16: 3571-3580.
52. Lee ES, Jeong MS, Singh R, Jung J, Yoon H, et al. (2012) A chimeric antibody to L1 cell adhesion molecule shows therapeutic effect in an intrahepatic cholangiocarcinoma model. Exp Mol Med 44: 293-302.
53. Schmid RS, Pruitt WM, Maness PF, (2000) A MAP kinase-signaling pathway mediates neurite outgrowth on L1 and requires Src-dependent endocytosis. J Neurosci 20: 4177-4188.
54. Guseva D, Zerwas M, Xiao M, Jakovcevski I, Irintchev A, et al. (2011) Adhesion molecule L1 overexpressed under the control of the neuronal Thy-1 promoter improves myelination after peripheral nerve injury in adult mice. Exp Neurol 229: 339-352.
55. Guseva D, Angelov DN, Irintchev A, Schachner M, (2009) Ablation of adhesion molecule L1 in mice favours Schwann cell proliferation and functional recovery after peripheral nerve injury. Brain 132: 2180-2195.