Engineering of human CYP3A enzymes by combination of activating polymorphic variants

Applied Biochemistry and Biotechnology

Volumn 168, Issue 4, 2012, Pages 785-796

  Neunzig, Ina ^a   Widjaja, Maria ^a   Drǎgan, Cǎlin Aurel ^a   Peters, Frank T ^b   Maurer, Hans H ^c   Bureik, Matthias ^a  

^a PomBioTech GmbH   (Germany)

^b JENA UNIVERSITY HOSPITAL   (Germany)

^c SAARLAND UNIVERSITY   (Germany)

Author keywords

Cytochrome P450; Enzyme engineering; Rational design; Site directed mutagenesis; Strain development; Whole cell biotransformation

Indexed keywords

CYTOCHROME P450; ENZYME ENGINEERING; RATIONAL DESIGN; SITE DIRECTED MUTAGENESIS; STRAIN DEVELOPMENT; WHOLE-CELL BIOTRANSFORMATIONS;

ENZYME ACTIVITY; GENETIC ENGINEERING; HYDROXYLATION; METABOLITES; SAFETY TESTING;

CELL ENGINEERING;

CYTOCHROME P450 3A; CYTOCHROME P450 3A4; CYTOCHROME P450 3A5; CYTOCHROME P450 3A7; PRASTERONE; TESTOSTERONE;

ARTICLE; BIOCATALYSIS; BIOTRANSFORMATION; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME ENGINEERING; GENETIC ENGINEERING; HUMAN; OXIDATION; RESTRICTION SITE; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; STEROID HYDROXYLATION; WILD TYPE; YEAST;

AMINO ACID SEQUENCE; BIOTRANSFORMATION; CYTOCHROME P-450 CYP3A; ENZYME ACTIVATION; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; PLASMIDS; POLYMORPHISM, GENETIC; PROTEIN ENGINEERING; SCHIZOSACCHAROMYCES;

SCHIZOSACCHAROMYCETACEAE;

EID: 84869144598     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-012-9819-0     Document Type: Article

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