High-throughput screening system based on phenolics-responsive transcription activator for directed evolution of organophosphate-degrading enzymes

Protein Engineering, Design and Selection

Volumn 25, Issue 11, 2012, Pages 725-731

  Jeong, Young Su ^a   Choi, Su Lim ^b   Kyeong, Hyun Ho ^a   Kim, Jin Hyun ^a   Kim, Eui Joong ^c   Pan, Jae Gu ^b   Rha, Eugene ^b   Song, Jae Jun ^b   Lee, Seung Goo ^b   Kim, Hak Sung ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^c GenoFocus Inc   (South Korea)

Author keywords

directed evolution; high throughput screening; organophosphate degrading enzymes; transcription activator

Indexed keywords

CATALYTIC EFFICIENCIES; DIRECTED EVOLUTION; ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE INTENSITIES; HIGH-THROUGHPUT SCREENING; HIGH-THROUGHPUT SCREENING SYSTEMS; HOST CELLS; HUMAN HEALTH PROBLEMS; METHYL PARATHION HYDROLASE; NERVE AGENTS; PHENOLIC COMPOUNDS; PSEUDOMONAS SP; SCREENING SYSTEM; TRANSCRIPTION ACTIVATORS; TRIPLE MUTANTS; WILD-TYPE ENZYMES;

DETOXIFICATION; ENZYMES; FLUORESCENCE; TRANSCRIPTION; TRANSCRIPTION FACTORS;

PHENOLS;

4 NITROPHENYLPHOSPHATASE; ENHANCED GREEN FLUORESCENT PROTEIN; ORGANOPHOSPHATE; PARATHION METHYL; PHENOL DERIVATIVE; TRANSCRIPTION FACTOR;

CATALYSIS; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME BINDING; ENZYME DEGRADATION; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE ACTIVATED CELL SORTING; HIGH THROUGHPUT SCREENING; HOST CELL; MOLECULAR CLONING; MOLECULAR EVOLUTION; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PSEUDOMONAS; STRUCTURE ANALYSIS; TRANSCRIPTION REGULATION;

BACTERIAL PROTEINS; CATALYTIC DOMAIN; CHEMICAL WARFARE AGENTS; DIRECTED MOLECULAR EVOLUTION; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MODELS, MOLECULAR; MUTATION; ORGANOPHOSPHATES; PESTICIDES; PHENOLS; PHOSPHORIC MONOESTER HYDROLASES; PROTEIN CONFORMATION; PSEUDOMONAS; SUBSTRATE SPECIFICITY; TRANS-ACTIVATORS;

PSEUDOMONAS SP. WBC-3;

EID: 84868579851     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs071     Document Type: Conference Paper

References (35)

1. Arnold, K., Bordoli, L., Kopp, J. and Schwede, T. (2006) Bioinformatics, 22, 195-201
2. Berendsen, H. J. C., Postma, J. P. M., DiNola, A. and Haak, J. R. (1984) J. Chem. Phys., 81, 3684-3690
3. Berendsen, H. J. C., Postma, J. P. M., van Gunsteren, W. F. and Hermans, J. (1981) In Pullman, B. (ed. ), Intermolecular Forces. D. Reidel Publishing Company, Dordrecht, pp. 331-342
4. Briseño-Roa, L., Hill, J., Notman, S., et al. (2006) J. Med. Chem., 49, 246-255
5. Chen, Y., Zhang, X., Liu, H., Wang, Y. and Xia, X. (2002) Acta Microbiol. Sin., 42, 490-497
6. Cheng, T. C., Harvey, S. P. and Chen, G. L. (1996) Appl. Environ. Microbiol., 62, 1636-1641
7. Cho, C. M., Mulchandani, A. and Chen, W. (2002) Appl. Environ. Microbiol., 68, 2026-2030
8. DeFrank, J. J. and Cheng, T. C. (1991) J. Bacteriol., 173, 1938-1943
9. DeLano, W. L. (2002) The PyMOL Molecular Graphics System. DeLano Scientific, San Carlos. http://pymol.sourceforge.net/
10. Di Sioudi, B. D., Miller, C. E., Lai, K., Grimsley, J. K. and Wild, J. R. (1999) Chem. Biol. Interact., 119-120, 211-223
11. Dong, Y. J., Bartlam, M., Sun, L., Zhou, Y. F., Zhang, Z. P., Zhang, C. G., Rao, Z. and Zhang, X. E. (2005) J. Mol. Biol., 353, 655-663
12. Griffiths, A. D. and Tawfik, D. S. (2003) EMBO J., 22, 24-35
13. Guex, N. and Peitsch, M. C. (1997) Electrophoresis, 18, 2714-2723
14. Hess, B., Kutzner, C., Van Der Spoel, D. and Lindahl, E. (2008) J. Chem. Theory Comput., 4, 435-447
15. Ibrahim, M. M. and Ramadan, A. M. (2010) J. Incl. Phenom. Macrocycl. Chem., 68, 287-296
16. Krivov, G. G., Shapovalov, M. V. and Dunbrack, R. L., Jr. (2009) Proteins: Struct. Funct. Bioinf., 77, 778-795
17. Menger, F. M. and Tsuno, T. (1989) J. Am. Chem. Soc., 111, 4903-4907
18. Mulbry, W. W., Karns, J. S., Kearney, P. C., Nelson, J. O., McDaniel, C. S. and Wild, J. R. (1986) Appl. Environ. Microbiol., 51, 926-930
19. Murphy, S. D. (1986) In Klaassen, C. D., Amdur, M. O. and Doull, J. (ed. ), Casarett and Doull's Toxicology, The Basic Science of Poisons. Macmillan Publishing. Co., New York, pp. 519-581
20. Omburo, G. A., Kuo, J. M., Mullins, L. S. and Raushel, F. M. (1992) J. Biol. Chem., 267, 13278-13283
21. Oostenbrink, C., Villa, A., Mark, A. E. and van Gunsteren, W. F. (2004) J. Comput. Chem., 25, 1656-1676
22. Pavel, H., Forsman, M. and Shingler, V. (1994) J. Bacteriol., 176, 7550-7557
23. Pope, C. N. (1999) J. Toxicol. Environ. Health Part B, 2, 161-181
24. Poo, H. R., Song, J. J., Hong, S. P., Choi, Y. H., Yun, S. W., Kim, J. H., Lee, S. C., Lee, S. G. and Sung, M. H. (2002) Biotechnol. Lett., 24, 1185-1189
25. Schwede, T., Kopp, J., Guex, N. and Peitsch, M. C. (2003) Nucleic Acids Res., 31, 3381-3385
26. Shimazu, M., Mulchandani, A. and Chen, W. (2001) Biotechnol. Prog., 17, 76-80
27. Shingler, V. and Moore, T. (1994) J. Bacteriol., 176, 1555-1560
28. Singh, B. K. and Walker, A. (2006) FEMS Microbiol. Rev., 30, 428-471
29. Tian, J., Wang, P., Gao, S., Chu, X., Wu, N. and Fan, Y. (2010) FEBS J., 277, 4901-4908
30. Tokuriki, N. and Tawfik, D. S. (2009) Nature, 459, 668-673
31. Tsai, P. C., Bigley, A., Li, Y., Ghanem, E., Cadieux, C. L., Kasten, S. A., Reeves, T. E., Cerasoli, D. M. and Raushel, F. M. (2010) Biochemistry, 49, 7978-7987
32. Van Der Spoel, D., Lindahl, E., Hess, B., Groenhof, G., Mark, A. E. and Berendsen, H. J. (2005) J. Comput. Chem., 26, 1701-1718
33. Vyas, N. K., Nickitenko, A., Rastogi, V. K., Shah, S. S. and Quiocho, F. A. (2010) Biochemistry, 49, 547-559
34. Yang, C., Cai, N., Dong, M., Jiang, H., Li, J., Qiao, C., Mulchandani, A. and Chen, W. (2008) Biotechnol. Bioeng., 99, 30-37
35. Yang, H., Carr, P. D., Yu-McLloughlin, S., et al. (2003) Protein, Eng, Des. Sel., 16, 135-145