Electromechanical deformation of mammalian cells in suspension depends on their cortical actin thicknesses

Journal of Biomechanics

Volumn 45, Issue 16, 2012, Pages 2797-2803

  MacQueen, Luke A ^a   Thibault, Marc ^a   Buschmann, Michael D ^a   Wertheimer, Michael R ^a  

^a ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

Author keywords

Cell mechanics; Cortical actin; Cytoskeleton; Deformability; Electro deformation

Indexed keywords

ACRYLAMIDES; CELL MECHANICS; CONFOCAL FLUORESCENT MICROSCOPY; CORTICAL ACTIN; CYTOSKELETONS; ELECTRO-DEFORMATION; ELECTROMECHANICAL DEFORMATION; HEK-293; HEK293 CELLS; LINEAR DEPENDENCE; LOADING CONDITION; MAMMALIAN CELLS; MAXIMAL STRAIN; MICROFILAMENTS; MOLECULAR NETWORKS; NONUNIFORM ELECTRIC FIELD; POWER LAW MODEL; POWER-LAW; STRAIN DATA; TIME VARYING; U937 CELL; YOUNG'S MODULUS;

AMIDES; CELLS; CYTOLOGY; DEFORMATION; FORMABILITY; LOADING; MECHANICAL PROPERTIES; PROTEINS;

BIOMECHANICS;

ACRYLAMIDE; ACTIN; LATRUNCULIN A; TOXIN;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CELL FUNCTION; CELL STRAIN HEK293; CELL STRAIN L 929; CELL STRAIN U937; CHO CELL; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ELECTRIC CONDUCTIVITY; ELECTRIC FIELD; FEMALE; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SHEAR STRESS; VISCOELASTICITY; YOUNG MODULUS;

ACTIN CYTOSKELETON; ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; ELASTICITY; ELECTRIC STIMULATION; HEK293 CELLS; HUMANS; MICE; STRESS, MECHANICAL; SUSPENSIONS; U937 CELLS; VISCOSITY;

EID: 84868337404     PISSN: 00219290     EISSN: 18732380     Source Type: Journal    
DOI: 10.1016/j.jbiomech.2012.08.040     Document Type: Article

References (41)

1. Ananthakrishnan R., Guck J., Wottawah F., Schinkinger S., Lincoln B., Romeyke M., Moon T., Ks J. Quantifying the contribution of actin networks to the elastic strength of fibroblasts. Journal of Theoretical Biology 2006, 242:502-516.
2. Ashkin A. Optical trapping and manipulation of neutral particles using lasers. Proceedings of the National Academy of Sciences of the United States of America 1997, 94:4853-4860.
3. Blanc A., Tran-Khanh N., Filion D., Buschmann M.D. Optimal processing method to obtain four-color confocal fluorescent images of the cytoskeleton and nucleus in three-dimensional chondrocyte cultures. Journal of Histochemistry and Cytochemistry 2005, 53(9):1171-1175.
4. Brakebusch C., Fässler R. The integrin-actin connection, an eternal love affair. The European Molecular Biology Organization Journal 2003, 22(10):2324-2333.
5. Bray D., White J.G. Cortical flow in animal cells. Science 1988, 239(4842):883-888.
6. Charras G.T., Hu C.-K., Coughlin M., Mitchison T.J. Reassembly of contractile actin cortex in cell blebs. Journal of Cell Biology 2006, 175(3):477-490.
7. Charras G.T., Coughlin M., Mitchison T.J., Mahadevan L. Life and times of a cellular bleb. Biophysical Journal 2008, 94:1836-1853.
8. Chen J., Zheng Y., Tan Q., Shojaei-Baghini E., Zhang Y.L., Li J., Prasad P., You L., Wu X.Y., Sun Y. Classification of cell types using a microfluidic device for mechanical and electrical measurement on single cells. Lab on a Chip 2011, 11:3174-3181.
9. Chowdhury F., Na S., Li D., Poh Y.C., Tanaka T., Wang F., Wang N. Material properties of the cell dictate stress-induced spreading and differentiation in embryonic stem cells. Nature Materials 2010, 9(1):82-88.
10. Correia I., Chu D., Chou Y.-H., Goldman R.D., Matsudaira P. Integrating the actin and vimentin cytoskeletons: adhesion-dependent formation of fimbrin-vimentin complexes in macrophages. Journal of Cell Biology 1999, 146:831-842.
11. Cuvelier D., Théry M., Chu Y.-S., Dufour S., Thiéry J.-P., Bornens M., Nassoy P., Mahadevan L. The universal dynamics of cell spreading. Current Biology 2007, 17:694-699.
12. Darling E.M., Topel M., Zauscher S., Vail T.P., Guilak F. Viscoelastic properties of human mesenchymally-derived stem cells, primary osteoblasts, chondrocytes and adipocytes. Journal of Biomechanics 2008, 41:454-464.
13. Desprat N., Richert A., Simeon J., Asnacios A. Creep function of a single living cell. Biophysical Journal 2005, 88:2224-2233.
14. Fernández P., Pullarkat P.A., Ott A. A master relation defines the nonlinear viscoelasticity of single fibroblasts. Biophysical Journal 2006, 90(10):3796-3805.
15. Fung Y.C. Biomechanics: Mechanical Properties of Living Tissues 1993, Springer-Verlag, New York. 2nd ed.
16. Gladilin E., Micoulet A., Hosseini B., Rohr K., Spatz J., Eils R. 3D finite element analysis of uniaxial cell stretching: from image to insight. Physical Biology 2007, 4(2):104-113.
17. Gossett D.R., Weaver W.M., Mach A.J., Hur S.C., Tse H.T., Lee W., Amini H., Di Carlo D. Label-free cell separation and sorting in microfluidic systems. Analytical and Bioanalytical Chemistry 2010, 397(8):3249-3267.
18. Graham F.L., Smiley J., Russell W.C., Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 1977, 36(1):59-74.
19. Guck J., Schinkinger S., Lincoln B., Wottawah F., Ebert S., Romeyke M., Lenz D., Erickson H.M., Ananthakrishnan R., Mitchell D., Käs J., Ulvick S., Bilby C. Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. Biophysical Journal 2005, 88(5):3689-3698.
20. Guilak F., Erickson G.R., Ping Ting-Beall H. The effects of osmotic stress on the viscoelastic and physical properties of articular chondrocytes. Biophysical Journal 2002, 82:720-727.
21. Hughes M.P. Nanoelectromechanics in Engineering and Biology 2003, CRC Press, Boca Raton.
22. Lang T., Wacker I., Wunderlich I., Rohrbach A., Giese G., Soldati T., Almers W. Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells. Biophysical Journal 2000, 78:2863-2877.
23. Lincoln B., Erickson H.M., Schinkinger S., Wottawah F., Mitchell D., Ulvick S., Bilby C., Guck J. Deformability-based flow cytometry. Cytometry A 2004, 59(2):203-209.
24. MacQueen L.A., Buschmann M.D., Wertheimer M.R. Mechanical properties of mammalian cells in suspension measured by electro-deformation. Journal of Micromechanics and Microengineering 2010, 20:065007.
25. Maloney J.M., Nikova D., Lautenschläger F., Clarke E., Langer R., Guck J., Van Vliet K.J. Mesenchymal stem cell mechanics from the attached to the suspended state. Biophysical Journal 2010, 99(8):2479-2487.
26. Maniotis A., Chen C., Ingber D.E. Demonstration of mechanical connections between integrins, cytoskeletal filaments and nucleoplasm that stabilize nuclear structure. Proceedings of the National Academy of Sciences of the United States of America 1997, 94:849-854.
27. Morone N., Fujiwara T., Murase K., Kasai R.S., Ike H., Yuasa S., Usukura J., Kusumi A. Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography. Journal of Cell Biology 2006, 174(6):851-862.
28. Ofek G., Wiltz D.C., Athanasiou K.A. Contribution of the cytoskeleton to the compressive properties and recovery behavior of single cells. Biophysical Journal 2009, 97:1873-1882.
29. Paluch E., van der Gucht J., Sykes C. Cracking up: symmetry breaking in cellular systems. Journal of Cell Biology 2006, 175(5):687-692.
30. Penman S. Rethinking cell structure. Proceedings of the National Academy of Sciences of the United States of America 1995, 92:5251-5257.
31. Pollard T.D., Blanchoin L., Mullins R.D. Molecular mechanisms controlling actin filament dynamics in nonmuscle cells. Annual Review of Biophysics and Biomolecular Structure 2000, 29:545-576.
32. Shaw G., Morse S., Ararat M., Graham F.L. Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. Federation of American Societies for Experimental Biology Journal 2002, 16(8):869-871.
33. Stricker J., Falzone T., Gardel M.L. Mechanics of the F-actin cytoskeleton. Journal of Biomechanics 2010, 43(1):9-14.
34. Sundstrom C., Nilsson K. Establishment and characterization of a human histiocytic lymphoma cell line (U-937). International Journal of Cancer 1976, 17:565-577.
35. Tan Y., Kong M., Chen S., Cheng S.H., Li R.A., Sun D. Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers. Journal of Biomechanics 2012, 45(1):123-128.
36. Teo S.K., Goryachev A.B., Parker K.H., Chiam K.H. Cellular deformation and intracellular stress propagation during optical stretching. Physical Review E 2010, 81(5):051924.
37. Thibault M.M., Buschmann M.D. Migration of bone marrow stromal cells in 3D: 4 color methodology reveals spatially and temporally coordinated events. Cell Motility and the Cytoskeleton 2006, 63(12):725-740.
38. Tinevez J.-Y., Schulze U., Salbreux G., Roensch J., Joanny J.-F., Paluch E. Role of cortical tension in bleb growth. Proceedings of the National Academy of Sciences of the United States of America 2009, 106(44):18581-18586.
39. Trickey W.R., Vail T.P., Guilak F. The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes. Journal of Orthopaedic Research 2004, 22(1):131-139.
40. Wang N., lngber D.E. Probing transmembrane mechanical coupling and cytomechanics using magnetic twisting cytometry. Biochemistry and Cell Biology 1995, 73(7-8):327-335.
41. Wong P.K., Tan W., Ho C.-M. Cell relaxation after electrodeformation: effect of latrunculin A on cytoskeletal actin. Journal of Biomechanics 2005, 38:529-535.