Optimal processing method to obtain four-color confocal fluorescent images of the cytoskeleton and nucleus in three-dimensional chondrocyte cultures

Journal of Histochemistry and Cytochemistry

Volumn 53, Issue 9, 2005, Pages 1171-1175

  Blanc, Antoine ^a   Tran Khanh, Nicolas ^a   Filion, Dominic ^a   Buschmann, Michael D ^a  

^a ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

Author keywords

Actin; Cartilage; Chondrocyte; Confocal microscopy; Cytoskeleton; Tubulin; Vimentin

Indexed keywords

ACTIN; DETERGENT; FIXATIVE; GLUTARALDEHYDE; OCTYLPOLYOXYETHYLENE; PARAFORMALDEHYDE; POLYOXYETHYLENE; TRITON X 100; TUBULIN; UNCLASSIFIED DRUG; VIMENTIN;

ACCURACY; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ARTICULAR CARTILAGE; CARTILAGE CELL; CELL CULTURE; CELL MEMBRANE PERMEABILITY; CELL NUCLEUS; CELL SIZE; CELL STRUCTURE; COLOR; CONFOCAL MICROSCOPY; CYTOSKELETON; FLUORESCENCE; NONHUMAN; OPTICAL RESOLUTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN LOCALIZATION; TISSUE ENGINEERING; TISSUE PREPARATION;

ACTINS; ANIMALS; CARTILAGE, ARTICULAR; CATTLE; CELL NUCLEUS; CELLS, CULTURED; CHONDROCYTES; CYTOSKELETON; FIXATIVES; FLUORESCENT ANTIBODY TECHNIQUE; FORMALDEHYDE; GLUTARAL; HYDROGELS; MICROSCOPY, CONFOCAL; OCTOXYNOL; POLYMERS; SEPHAROSE; TISSUE FIXATION; TUBULIN; VIMENTIN;

EID: 24344476232     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.5B6728.2005     Document Type: Article

References (10)

1. Arcangeletti C, Sutterlin R, Aebi U, De Conto F, Missorini S, Chezzi C, Scherrer K (1997) Visualization of prosomes (MCP-proteasomes), intermediate filament and actin networks by "instantaneous fixation" preserving the cytoskeleton. J Struct Biol 119:35-58
2. Bacallao R, Kiai K, Jesaitis L (1995) Guiding principles of specimen preservation for confocal fluorescence microscopy. In Pawley JB, ed. Handbook of Biological Confocal Microscopy. New York, Plenum Press, 311-325
3. Dickinson ME, Bearman G, Tille S, Lansford R, Fraser SE (2001) Multi-spectral imaging and linear unmixing add a whole new dimension to laser scanning fluorescence microscopy. BioTechniques 31:1272-1278
4. Dumont J, Ionescu M, Reiner A, Poole AR, Tran-Khanh N, Hoemann CD, McKee MD, et al. (1999) Mature full-thickness articular cartilage explants attached to bone are physiologically stable over long-term culture in serum-free media. Connect Tissue Res 40:259-272
5. Durrant LA, Archer CW, Benjamin M, Ralphs JR (1999) Organisation of the chondrocyte cytoskeleton and its response to changing mechanical conditions in organ culture. J Anat 194:343-353
6. Idowu BD, Knight MM, Bader DL, Lee DA (2000) Confocal analysis of cytoskeletal organisation within isolated chondrocyte subpopulations cultured in agarose. Histochem J 32:165-174
7. Langelier E, Suetterlin R, Hoemann CD, Aebi U, Buschmann MD (2000) The chondrocyte cytoskeleton in mature articular cartilage: structure and distribution of actin, tubulin, and vimentin filaments. J Histochem Cytochem 48:1307-1320
8. Szuts V, Mollers U, Bittner K, Schurmann G, Muratoglu S, Deak F, Kiss I, et al. (1998) Terminal differentiation of chondrocytes is arrested at distinct stages identified by their expression repertoire of marker genes. Matrix Biol 17:435-448
9. Tran-Khanh N, Hoemann CD, McKee MD, Henderson JE, Buschmann MD (2005) Aged bovine chondrocytes display a diminished capacity to produce a collagen-rich, mechanically functional cartilage extracellular matrix. J Orthop Res in press
10. Trickey WR, Vail TP, Guilak F (2004) The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes. J Orthop Res 22:131-139