Design and assembly of large synthetic DNA constructs

Current Protocols in Molecular Biology

Volumn 1, Issue SUPPL.99, 2012, Pages

  Miklos, Aleksandr E ^a   Hughes, Randall A ^a   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Gene assembly; Gene synthesis; Inside out nucleation; Oligonucleotide assembly; Oligonucleotide synthesis; Oligonucleotides; PCR; Protein expression; Synthetic biology; Synthetic DNA; Synthetic genes

Indexed keywords

GREEN FLUORESCENT PROTEIN; OLIGONUCLEOTIDE; SYNTHETIC DNA; PROTEIN;

AGAR GEL ELECTROPHORESIS; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; AT RICH SEQUENCE; CLINICAL PROTOCOL; CODON USAGE; DNA BASE COMPOSITION; DNA SEQUENCE; ESCHERICHIA COLI; EXPERIMENTAL DESIGN; GC RICH SEQUENCE; GENE EXPRESSION REGULATION; GENE SYNTHESIS; GENETIC VARIABILITY; IN VITRO STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; REACTION ANALYSIS; REACTION TIME; SEQUENCE ANALYSIS; SYNTHETIC BIOLOGY; CHEMISTRY; GENETICS; METHODOLOGY; SYNTHESIS; WORKFLOW;

AMINO ACID SEQUENCE; GENES, SYNTHETIC; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; PROTEINS; SYNTHETIC BIOLOGY; WORKFLOW;

EID: 84868113328     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb0323s99     Document Type: Article

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