Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy

Biophysical Journal

Volumn 73, Issue 5, 1997, Pages 2782-2790

  Patterson, George H ^a   Knobel, Susan M ^a   Sharif, Wallace D ^a   Kain, Steven R ^b   Piston, David W ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CLONTECH Laboratories Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; MUTANT PROTEIN;

ARTICLE; CHROMATOPHORE; CONTROLLED STUDY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; NONHUMAN;

EID: 0030784698     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(97)78307-3     Document Type: Article

References (35)

1. Berlman, I. B. 1971. Handbook of Fluorescence Spectra of Aromatic Molecules. Academic Press, New York.
2. Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science. 263:802-805.
3. Chattoraj, M., B. A. King, G. U. Bublitz, and S. G. Boxer. 1996. Ultra-fast excited state dynamics in green fluorescent protein: multiple states and proton transfer. Proc. Natl. Acad. Sci. USA. 93:8362-8367.
4. Chiu, W., Y. Niwa, W. Zeng, T. Hirano, H. Kobayashi, and J. Sheen. 1996. Engineered GFP as a vital reporter in plants. Curr. Biol. 6:325-330.
5. Cormack, B. P., G. Bertram, M. Egerton, N. A. R. Gow, S. Falkow, and A. J. P. Brown. 1997. Green fluorescent protein (GFP): a marker for gene expression in Candida albicans. Microbiolology. 143(Part2): 303-311.
6. Cormack, B. P., R. H. Valdivia, and S. Falkow. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 173:33-38.
7. Crameri, A., E. A. Whitchorn, F., Tate, and W. P. C. Stemmer. 1996. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotechnol. 14:315-319.
8. Cubitt, A. B., R. Heim, S. R. Adams, A. E. Boyd, L. A. Gross, and R. Y. Tsien. 1995. Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20:448-455.
9. Gerdes, H.-H., and C. Kaether. 1996. Green fluorescent protein: applications in biology. FEBS Lett. 389:44-47.
10. Haas, J., E.-C. Park, and B. Seed. 1996. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr. Biol. 6:315-324.
11. Heim, R., A. B. Cubitt, and R. Y. Tsien. 1995. Improved green fluorescence. Nature. 373:663-664.
12. Heim, R., D. C. Prasher, and R. Y. Tsien. 1994. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA. 91:12501-12504.
13. Heim, R., and R. Y. Tsien. 1996. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol. 6:178-182.
14. Herman, B. 1989. Resonance energy transfer microscopy. In Fluorescence Microscopy of Living Cells in Culture. D. L. Taylor and Y. Wang, editors. Academic Press, San Diego. CA. 220-243.
15. Inouye, S., and F. I. Tsuji. 1994. Aequorea green fluorescent protein: expression of the gene and fluorescent characteristics of the recombinant protein. FEBS Lett. 341:277-280.
16. Kaether, C., and H. Gerdes. 1995. Visualisation of protein transport along the secretory pathway using green fluorescent protein. FEBS Lett. 369:267-271.
17. Luhy-Phelps, K., D. L. Taylor, and F. Lanni. 1986. Probing the structure of the cytoplasm. J. Cell Biol. 102:2015-2022.
18. Mellman, I., R. Fuchs, and A. Helenius. 1986. Acidification of the endocytic and exocytic pathways. Annu. Rev. Biochem. 55:663-700.
19. Mitra, R. D., C. M. Silva, and D. C. Youvan. 1996. Fluorescence resonance energy transfer between blue-emitling and red-shifted excitation derivatives of the green fluorescent protein. Gene. 173:13-17.
20. Morise, H., O. Shimomura, F. H. Johnson, and J. Winant. 1974. Intermolecular energy transfer in the biolumincscent system of Aquorea. Biochemistry. 13:2656-2662.
21. Niswender, K. D., S. M. Blackman, L. Rohde, M. A. Magnuson, and D. W. Piston. 1995. Quantitative imaging of green fluorescent protein in cultured cells: comparison of microscopic techniques, use in fusion proteins and detection limits. J. Microsc. 180:109-116.
22. Ogawa, H., S. Inouye, F. I. Tsuji, K. Yasuda and K. Umesono. 1995. Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells. Proc. Natl. Acad. Sci. USA. 92:11899-11903.
23. Ormo, M., A. B. Cubitt, K. Kallio, L. A. Gross, R. Y. Tsien, and S. J. Remington. 1996. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 273:1392-1395.
24. Piston, D. W., B. D. Bennett, and G. Ying. 1995. Imaging of cellular dynamics by two-photon excitation microscopy. Microsc. Microanal. 1:25-34.
25. Prasher, D. C., V. K. Eckenrode, W. W. Ward, F. G. Prendergast, and M. J. Cormier. 1992. Primary structure of the Aequorca victoria green-fluorescent protein. Gene. 111:229-233.
26. Rizzuto, R., M. Brini, F. D. Giorgi, R. Rossi, R. Heim, R. Y. Tsien, and T. Pozzan. 1996. Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo. Curr. Biol. 6:183-188.
27. Sambrook, J., H. F. Fritsch, and T. Maniatis. 1989a. Appendix A: bacterial media, antibiotics, and bacterial strains. In Molecular Cloning: A laboratory Manual. C. Nolan, editor. Cold Spring Harbor Laboratory. Cold Spring Harbor. NY. A.2.
28. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989b. Site-directed mutagensis of cloned DNA. In Molecular Cloning: A Laboratory Manual. C. Nolan, editor. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 15.74-15.79.
29. Sandison, D. R., D. W. Piston, R. M. Williams and W. W. Webb. 1995. Resolution, background rejection, and signal-to-noise in widefield and confocal microscopy. Appl. Optics. 34:3576-3588.
30. Siemering, K. R., R. Golbik, R. Sever, and J. Haseloff. 1996. Mutations that suppress the thermosensitivity of green fluorescent protein. Curr. Biol. 6:1653-1663.
31. Studier, F. W., A. H. Rosenberg, J. J. Dunn and J. W. Dubendorff. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. D. V. Goeddel, editor. Academic Press. San Diego, CA. 60-89.
32. Ward, W. W. 1981. Properties of the coelenterate green-fluorescent protein. In Bioluminescence and Chemiluminesccnce. M. A. DeLuca and W. D. McElroy, editors. Academic Press, New York. 235-242.
33. Ward, W. W., H. J. Prentice, A. F. Roth, C. W. Cody, and S. C. Reeves. 1982. Spectral perturbations of the Aequorea green-fluorescent protein. Photochem. Photobiol 35:803-808.
34. Xu, C., W. Zipfel, J. B. Shear, R. M. Williams, and W. W. Webb. 1996 Mutliphoton fluorescence excitation: new spectral windows for nonlinear microscopy. Proc. Natl. Acad. Sci. USA. 93:10763-10768.
35. Yang, T.-T L. Cheng, and S. R. Kain. 1996. Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein. Nucleic Acids Res. 24:4592-4593.