Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences.

Nucleic acids research

Volumn 31, Issue 22, 2003, Pages

  Gao, Xinxin ^a   Yo, Peggy ^a   Keith, Andrew ^a   Ragan, Timothy J ^a   Harris, Thomas K ^a  

^a Department of Biology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3 PHOSPHOINOSITIDE DEPENDENT PROTEIN KINASE; 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE; DNA; ONCOPROTEIN; PRIMER DNA; PROTEIN KINASE B; PROTEIN SERINE THREONINE KINASE; S6 KINASE; SYNTHETIC DNA;

AMINO ACID SEQUENCE; ARTICLE; GENETICS; HUMAN; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SYNTHESIS; THERMODYNAMICS;

AMINO ACID SEQUENCE; BASE SEQUENCE; DNA; DNA PRIMERS; GENES, SYNTHETIC; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-AKT; REPRODUCIBILITY OF RESULTS; RIBOSOMAL PROTEIN S6 KINASES, 70-KDA; THERMODYNAMICS;

MLCS; MLOWN;

EID: 2142799564     PISSN: None     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gng143     Document Type: Article

References (0)