The PNT domain from Drosophila pointed-P2 contains a dynamic N-terminal helix preceded by a disordered phosphoacceptor sequence

Protein Science

Volumn 21, Issue 11, 2012, Pages 1716-1725

  Lau, Desmond K W ^a   Okon, Mark ^a   McIntosh, Lawrence P ^a,b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Hydrogen exchange; MAP kinase docking; NMR spectroscopy; Phosphorylation; protein dynamics; PNT domain; Ras dependent signal transduction; SAM domain; Transcription

Indexed keywords

AMIDE; DROSOPHILA PROTEIN; HYDROGEN; MITOGEN ACTIVATED PROTEIN KINASE 1; THREONINE; TRANSCRIPTION FACTOR ETS 1; TRANSCRIPTION FACTOR ETS 2;

AMINO TERMINAL SEQUENCE; ARTICLE; DNA BINDING; DROSOPHILA; ENZYME PHOSPHORYLATION; IN VITRO STUDY; MOLECULAR DOCKING; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; SIGNAL TRANSDUCTION;

ANIMALS; DNA-BINDING PROTEINS; DROSOPHILA; DROSOPHILA PROTEINS; MITOGEN-ACTIVATED PROTEIN KINASE 1; MODELS, MOLECULAR; NERVE TISSUE PROTEINS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTO-ONCOGENE PROTEINS; RECOMBINANT PROTEINS; TRANSCRIPTION FACTORS;

EID: 84867668538     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2151     Document Type: Article

References (47)

1. Hollenhorst PC, McIntosh LP, Graves BJ (2010) Genomic and biochemical insights into the specificity of ETS transcription factors. Annu Rev Biochem 80:437-471.
2. Qiao F, Bowie JU (2005) The many faces of SAM. Sci STKE 2005:re7.
3. Mackereth CD, Scharpf M, Gentile LN, MacIntosh SE, Slupsky CM, McIntosh LP (2004) Diversity in structure and function of the Ets family PNT domains. J Mol Biol 342:1249-1264. (Pubitemid 39213931)
4. Nelson ML, Kang HS, Lee GM, Blaszczak AG, Lau DK, McIntosh LP, Graves BJ (2010) Ras signaling requires dynamic properties of ETS1 for phosphorylation-enhanced binding to coactivator CBP. Proc Natl Acad Sci USA 107:10026-10031.
5. Meruelo AD, Bowie JU (2009) Identifying polymerforming SAM domains. Proteins 74:1-5.
6. Kim CA, Phillips ML, Kim W, Gingery M, Tran HH, Robinson MA, Faham S, Bowie JU (2001) Polymerization of the SAM domain of TEL in leukemogenesis and transcriptional repression. EMBO J 20:4173-4182. (Pubitemid 32751833)
7. Qiao F, Song H, Kim CA, Sawaya MR, Hunter JB, Gingery M, Rebay I, Courey AJ, Bowie JU (2004) Derepression by depolymerization; structural insights into the regulation of Yan by Mae. Cell 118:163-173. (Pubitemid 38962573)
8. Klambt C (1993) The Drosophila gene Pointed encodes two Ets-like proteins which are involved in the development of the midline glial cells. Development 117:163-176.
9. Wasylyk C, Bradford AP, Gutierrez-Hartmann A, Wasylyk B (1997) Conserved mechanisms of Ras regulation of evolutionary related transcription factors, Ets1 and Pointed P2. Oncogene 14:899-913. (Pubitemid 27122422)
10. Seidel JJ, Graves BJ (2002) An ERK2 docking site in the Pointed domain distinguishes a subset of Ets transcription factors. Genes Dev 16:127-137. (Pubitemid 34049643)
11. Rainey MA, Callaway K, Barnes R, Wilson B, Dalby KN (2005) Proximity-induced catalysis by the protein kinase ERK2. J Am Chem Soc 127:10494-10495. (Pubitemid 41061416)
12. Abramczyk O, Rainey MA, Barnes R, Martin L, Dalby KN (2007) Expanding the repertoire of an ERK2 recruitment site: cysteine footprinting identifies the D-recruitment site as a mediator of Ets-1 binding. Biochemistry 46:9174-9186. (Pubitemid 47255032)
13. Callaway KA, Rainey MA, Riggs AF, Abramczyk O, Dalby KN (2006) Properties and regulation of a transiently assembled ERK2•Ets-1 signaling complex. Biochemistry 45:13719-13733. (Pubitemid 44788742)
14. Callaway K, Waas WF, Rainey MA, Ren PY, Dalby KN (2010) Phosphorylation of the transcription factor Ets-1 by ERK2: rapid dissociation of ADP and phospho-Ets-1. Biochemistry 49:3619-3630.
15. Piserchio A, Warthaka M, Devkota AK, Kaoud TS, Lee S, Abramczyk O, Ren P, Dalby KN, Ghose R (2011) Solution NMR insights into docking interactions involving inactive Erk2. Biochemistry 50:3660-3672.
16. Lee S, Warthaka M, Yan C, Kaoud TS, Piserchio A, Ghose R, Ren P, Dalby KN (2011) A model of a MAPK*substrate complex in an active conformation: a computational and experimental approach. PLoS One 6:e18594.
17. Foulds CE, Nelson ML, Blaszczak AG, Graves BJ (2004) Ras/mitogen- activated protein kinase signaling activates Ets-1 and Ets-2 by CBP/p300 recruitment. Mol Cell Biol 24:10954-10964. (Pubitemid 39603149)
18. Brunner D, Ducker K, Oellers N, Hafen E, Scholz H, Klambt C (1994) The Ets domain protein pointed-P2 is a target of MAP kinase in the sevenless signal transduction pathway. Nature 370:386-389. (Pubitemid 24259764)
19. O'Neill EM, Rebay I, Tjian R, Rubin GM (1994) The activities of two Ets-related transcription factors required for Drosophila eye development are modulated by the Ras/MAPK pathway. Cell 78:137-147. (Pubitemid 24228308)
20. Tootle TL, Rebay I (2005) Post-translational modifications influence transcription factor activity: a view from the Ets superfamily. Bioessays 27:285-298. (Pubitemid 40476491)
21. Vivekanand P, Rebay I (2006) Intersection of signal transduction pathways and development. Annu Rev Genet 40:139-157. (Pubitemid 44956782)
22. Vivekanand P, Tootle TL, Rebay I (2004) Mae, a dual regulator of the EGFR signaling pathway, is a target of the Ets transcription factors PNT and YAN. Mech Dev 121:1469-1479. (Pubitemid 39423626)
23. Tootle TL, Lee PS, Rebay I (2003) Crm1-mediated nuclear export and regulated activity of the receptor tyrosine kinase antagonist YAN require specific interactions with Mae. Development 130:845-857. (Pubitemid 36367506)
24. Song H, Nie M, Qiao F, Bowie JU, Courey AJ (2005) Antagonistic regulation of YAN nuclear export by Mae and Crm1 may increase the stringency of the Ras response. Genes Dev 19:1767-1772.
25. Baker DA, Mille-Baker B, Wainwright SM, Ish-Horowicz D, Dibb NJ (2001) Mae mediates MAP kinase phosphorylation of Ets transcription factors in Drosophila. Nature 411:330-334. (Pubitemid 32467041)
26. Qiao F, Harada B, Song H, Whitelegge J, Courey AJ, Bowie JU (2006) Mae inhibits Pointed-p2 transcriptional activity by blocking its MAPK docking site. EMBO J 25:70-79. (Pubitemid 43077296)
27. Vivekanand P, Rebay I The SAM domain of human TEL2 can abrogate transcriptional output from TEL1 (ETV-6) and ETS1/ETS2. PLoS One 7:e37151.
28. Shen Y, Bax A (2012) Identification of helix capping and β-turn motifs from NMR chemical shifts. J Biomol NMR 52:211-232.
29. Farrow NA, Muhandiram R, Singer AU, Pascal SM, Kay CM, Gish G, Shoelson SE, Pawson T, Forman-Kay JD, Kay LE (1994) Backbone dynamics of a free and phosphopeptide-complexed src homology 2 domain studied by 15N NMR relaxation. Biochemistry 33:5984-6003. (Pubitemid 24184605)
30. Kleckner IR, Foster MP (2011) An introduction to NMR-based approaches for measuring protein dynamics. Biochim Biophys Acta-Proteins Proteomics 1814:942-968.
31. Bai Y, Milne JS, Mayne L, Englander SW (1993) Primary structure effects on peptide group hydrogen exchange. Proteins 17:75-86. (Pubitemid 23261059)
32. 31P spin-echo difference NMR spectroscopy. J Biomol NMR 43:31-37.
33. Bienkiewicz EA, Lumb KJ (1999) Random-coil chemical shifts of phosphorylated amino acids. J Biomol NMR 15:203-206. (Pubitemid 30016118)
34. Gonzalez FA, Raden DL, Davis RJ (1991) Identification of substrate recognition determinants for human ERK1 and ERK2 protein-kinases. J Biol Chem 266:22159-22163. (Pubitemid 21908629)
35. Songyang Z, Lu KP, Kwon YT, Tsai LH, Filhol O, Cochet C, Brickey DA, Soderling TR, Bartleson C, Graves DJ, DeMaggio AJ, Hoekstra MF, Blenis J, Hunter T, Cantley LC (1996) A structural basis for substrate specificities of protein ser/thr kinases: primary sequence preference of casein kinases I and II, NIMA, phosphorylase kinase, calmodulin-dependent kinase II, CDK5, and ERK1. Mol Cell Biol 16:6486-6493. (Pubitemid 26361007)
36. Andrew CD, Warwicker J, Jones GR, Doig AJ (2002) Effect of phosphorylation on alpha-helix stability as a function of position. Biochemistry 41:1897-1905. (Pubitemid 34132265)
37. Arnold K, Bordoli L, Kopp J, Schwede T (2006) The Swiss-model workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22:195-201. (Pubitemid 43205406)
38. DeLano WL (2004) Use of Pymol as a communications tool for molecular science. Abstracts of Papers of the American Chemical Society 228:U313-U314.
39. Mackereth CD (2003) Structural and functional characterization of the PNT and SAM domains: diverse roles in protein-protein interactions. PhD Thesis, University of British Columbia.
40. Tanoue T, Adachi M, Moriguchi T, Nishida E (2000) A conserved docking motif in MAP kinases common to substrates, activators and regulators. Nat Cell Biol 2:110-116.
41. Kumar JP, Jamal T, Doetsch A, Turner FR, Duffy JB (2004) CREB binding protein functions during successive stages of eye development in Drosophila. Genetics 168:877-893. (Pubitemid 39491465)
42. De Guzman RN, Wojciak JM, Martinez-Yamout MA, Dyson HJ, Wright PE (2005) Cbp/p300 TAZ1 domain forms a structured scaffold for ligand binding. Biochemistry 44:490-497. (Pubitemid 40105479)
43. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NNMpipe - a multidimensional spectral processing system based on Unix pipes. J Biomol NMR 6:277-293.
44. Goddard TD, Kneeler DG (1999) Sparky, 3rd ed. San Francisco: University of California.
45. Sattler M, Schleucher J, Griesinger C (1999) Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Prog Nucl Mag Reson Spect 34:93-158. (Pubitemid 129595216)
46. Dosset P, Hus JC, Blackledge M, Marion D (2000) Efficient analysis of macromolecular rotational diffusion from heteronuclear relaxation data. J Biomol NMR 16:23-28. (Pubitemid 30114721)
47. Hwang TL, van Zijl PC, Mori S (1998) Accurate quantitation of water-amide proton exchange rates using the phase-modulated clean chemical exchange (CLEANEX-PM) approach with a fast-HSQC (fHSQC) detection scheme. J Biomol NMR 11:221-226.