Improving the thermostability of lipase Lip2 from Yarrowia lipolytica

Journal of Biotechnology

Volumn 164, Issue 2, 2012, Pages 248-253

  Wen, Sai ^a,b   Tan, Tianwei ^a   Zhao, Huimin ^b  

^a BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY   (China)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

B FIT; Directed evolution; Protein engineering; Semi rational design; Thermostability

Indexed keywords

B-FIT; COMPLEMENTARY PROTEINS; DIRECTED EVOLUTION; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS; THERMAL INACTIVATION; THERMOSTABILITY; YARROWIA LIPOLYTICA LIPASE;

BIOCHEMICAL ENGINEERING; GENETIC ENGINEERING;

LIPASES;

TLLIP2; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYST; CONTROLLED STUDY; ENZYME METABOLISM; ENZYME STABILITY; GENETIC VARIABILITY; MOLECULAR EVOLUTION; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN STRUCTURE; THERMOSTABILITY; WILD TYPE; YARROWIA LIPOLYTICA;

DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; ESCHERICHIA COLI; FUNGAL PROTEINS; HOT TEMPERATURE; KINETICS; LIPASE; MODELS, MOLECULAR; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; YARROWIA;

YARROWIA LIPOLYTICA;

EID: 84867089000     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.08.023     Document Type: Article

References (47)

1. Acharya P., Rajakumara E., Sankaranarayanan R., Rao N.M. Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase. Journal of Molecular Biology 2004, 341:1271-1281.
2. Ahmad S., Kamal M.Z., Sankaranarayanan R., Rao N.M. Thermostable Bacillus subtilis lipases: in vitro evolution and structural insight. Journal of Molecular Biology 2008, 381:324-340.
3. Anbar M., Lamed R., Bayer E.A. Thermostability enhancement of Clostridium thermocellum cellulosomal endoglucanase Cel8A by a single glycine substitution. ChemCatChem 2010, 2:997-1003.
4. Arnold F.H., Volkov A.A. Directed evolution of biocatalysts. Current Opinion in Chemical Biology 1999, 3:54-59.
5. Bommarius A.S., Broering J.M., Chaparro-Riggers J.F., Polizzi K.M. High-throughput screening for enhanced protein stability. Current Opinion in Biotechnology 2006, 17:606-610.
6. Bordes F., Barbe S., Escalier P., Mourey L., André I., Marty A., Tranier S. Exploring the conformational states and rearrangements of Yarrowia lipolytica lipase. Biophysical Journal 2010, 99:2225-2234.
7. Bordes F., Cambon E., Dossat-Létisse V., André I., Croux C., Nicaud J.M., Marty A. Improvement of Yarrowia lipolytica lipase enantioselectivity by using mutagenesis targeted to the substrate binding site. ChemBioChem 2009, 10:1705-1713.
8. Bordes F., Tarquis L., Nicaud J.-M., Marty A. Isolation of a thermostable variant of Lip2 lipase from Yarrowia lipolytica by directed evolution and deeper insight into the denaturation mechanisms involved. Journal of Biotechnology 2011, 156:117-124.
9. Bornscheuer U.T. Immobilizing enzymes: how to create more suitable biocatalysts. Angewandte Chemie International Edition 2003, 42:3336-3337.
10. Bornscheuer U.T., Pohl M. Improved biocatalysts by directed evolution and rational protein design. Current Opinion in Chemical Biology 2001, 5:137-143.
11. Cambon E., Piamtongkam R., Bordes F., Duquesne S., André I., Marty A. Rationally engineered double substituted variants of Yarrowia lipolytica lipase with enhanced activity coupled with highly inverted enantioselectivity towards 2-bromo phenyl acetic acid esters. Biotechnology and Bioengineering 2010, 106:852-859.
12. Chodorge M., Fourage L., Ullmann C., Duvivier V., Masson J.-M., Lefèvre F. Rational strategies for directed evolution of biocatalysts - application to Candida antarctica lipase B (CALB). Advanced Synthesis and Catalysis 2005, 347:1022-1026.
13. Clark E.D.B. Refolding of recombinant proteins. Current Opinion in Biotechnology 1998, 9:157-163.
14. Clark E.D.B. Protein refolding for industrial processes. Current Opinion in Biotechnology 2001, 12:202-207.
15. Deng L., Tan T., Wang F., Xu X. Enzymatic production of fatty acid alkyl esters with a lipase preparation from Candida sp 99-125. European Journal of Lipid Science and Technology 2003, 105:727-734.
16. Fickers P., Marty A., Nicaud J.M. The lipases from Yarrowia lipolytica: genetics, production, regulation, biochemical characterization and biotechnological applications. Biotechnology Advances 2011, 29:632-644.
17. Fu D., Li C., Lu J., Rahman A., Tan T.W. Relationship between thermal inactivation and conformational change of Yarrowia lipolytica lipase and the effect of additives on enzyme stability. Journal of Molecular Catalysis B: Enzymatic 2010, 66:136-141.
18. Guieysse D., Sandoval G., Faure L., Nicaud J.-M., Monsan P., Marty A. New efficient lipase from Yarrowia lipolytica for the resolution of 2-bromo-arylacetic acid esters. Tetrahedron: Asymmetry 2004, 15:3539-3543.
19. Haruki M., Tanaka M., Motegi T., Tadokoro T., Koga Y., Takano K., Kanaya S. Structural and thermodynamic analyses of Escherichia coli RNase HI variant with quintuple thermostabilizing mutations. FEBS Journal 2007, 274:5815-5825.
20. Jochens H., Aerts D., Bornscheuer U.T. Thermostabilization of an esterase by alignment-guided focussed directed evolution. Protein Engineering, Design and Selection 2010, 23:903-909.
21. Kamal M.Z., Ahmad S., Molugu T.R., Vijayalakshmi A., Deshmukh M.V., Sankaranarayanan R., Rao N.M. In vitro evolved non-aggregating and thermostable lipase: structural and thermodynamic investigation. Journal of Molecular Biology 2011, 413:726-741.
22. Kimura S., Kanaya S., Nakamura H. Thermostabilization of Escherichia coli ribonuclease HI by replacing left-handed helical Lys95 with Gly or Asn. Journal of Biological Chemistry 1992, 267:22014-22017.
23. Leblond, Y., Mouz, N., Marty, A., Uribelarrea, J.-L., 2012. Method for producing lipase transformed Yarrowia lipolytica cell capable of producing said lipase and their uses. USPTO 20120003716.
24. Neurath H. The role of glycine in protein structure. Journal of the American Chemical Society 1943, 65:2039-2041.
25. Nie K., Xie F., Wang F., Tan T. Lipase catalyzed methanolysis to produce biodiesel: optimization of the biodiesel production. Journal of Molecular Catalysis B: Enzymatic 2006, 43:142-147.
26. O'Fagain C. Enzyme stabilization-recent experimental progress. Enzyme and Microbial Technology 2003, 33:137-149.
27. Pan X., Chen B., Wang J., Zhang X., Zhul B., Tan T. Enzymatic synthesizing of phytosterol oleic esters. Applied Biochemistry and Biotechnology 2011, 1-10.
28. Pettersen E., Goddard T., Huang C., Couch G., Greenblatt D., Meng E., Ferrin T. UCSF Chimera-a visualization system for exploratory research and analysis. Journal of Computational Chemistry 2004, 25:1605-1612.
29. Reetz M.T., Carballeira J.D. Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes. Nature Protocols 2007, 2:891-903.
30. Reetz M.T., Carballeira J.D., Vogel A. Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability. Angewandte Chemie International Edition 2006, 45:7745-7751.
31. Reetz M.T., Soni P., Acevedo J.P., Sanchis J. Creation of an amino acid network of structurally coupled residues in the directed evolution of a thermostable enzyme. Angewandte Chemie International Edition 2009, 48:8268-8272.
32. Reetz M.T., Soni P., Fernández L. Knowledge-guided laboratory evolution of protein thermolability. Biotechnology and Bioengineering 2009, 102:1712-1717.
33. Reetz M.T., Soni P., Fernandez L., Gumulya Y., Carballeira J.D. Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method. Chemical Communications 2010, 46:8657-8658.
34. Reetz M.T., Tielmann P., Wiesenhöfer W., Könen W., Zonta A. Second generation sol-gel encapsulated lipases: robust heterogeneous biocatalysts. Advanced Synthesis and Catalysis 2003, 345:717-728.
35. Sanchis J., Fernández L., Carballeira J., Drone J., Gumulya Y., Höbenreich H., Kahakeaw D., Kille S., Lohmer R., Peyralans J., Podtetenieff J., Prasad S., Soni P., Taglieber A., Wu S., Zilly F., Reetz M. Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates. Applied Microbiology and Biotechnology 2008, 81:387-397.
36. Sylvestre J., Chautard H., Cedrone F., Delcourt M. Directed evolution of biocatalysts. Organic Process Research and Development 2006, 10:562-571.
37. Tairo O. Stabilization of proteins by evolutionary molecular engineering techniques. Current Opinion in Structural Biology 1994, 4:623-628.
38. Tan T., Chen B.-q., Ye H. Enzymatic synthesis of 2-ethylhexyl palmitate by lipase immobilized on fabric membranes in the batch reactor. Biochemical Engineering Journal 2006, 29:41-45.
39. Tan T., Nie K., Wang F. Production of biodiesel by immobilized Candida sp. lipase at high water content. Applied Biochemistry and Biotechnology 2006, 128:109-116.
40. Trevino S.R., Schaefer S., Scholtz J.M., Pace C.N. Increasing protein conformational stability by optimizing β-turn sequence. Journal of Molecular Biology 2007, 373:211-218.
41. Visser, D.F., 2010. Isolation and Evolution of Novel Nucleoside Phosphorylases. Ph.D. Thesis, Rhodes University, Rhodes, 285 p.
42. Wen S., Tan T., Yu M. Immobilized lipase YlLip2-catalyzed resolution of (±)α-phenylethyl amine in a medium with organic cosolvent. Process Biochemistry 2008, 43:1259-1264.
43. Yi Z.-L., Pei X.-Q., Wu Z.-L. Introduction of glycine and proline residues onto protein surface increases the thermostability of endoglucanase CelA from Clostridium thermocellum. Bioresource Technology 2011, 102:3636-3638.
44. Yu M., Lange S., Richter S., Tan T., Schmid R.D. High-level expression of extracellular lipase Lip2 from Yarrowia lipolytica in Pichia pastoris and its purification and characterization. Protein Expression and Purification 2007, 53:255-263.
45. Yu M., Wen S., Tan T. Enhancing production of Yarrowia lipolytica lipase Lip2 in Pichia pastoris. Engineering in Life Sciences 2010, 10:458-464.
46. Zhang N., Suen W.C., Windsor W., Xiao L., Madison V., Zaks A. Improving tolerance of Candida antarctica lipase B towards irreversible thermal inactivation through directed evolution. Protein Engineering 2003, 16:599-605.
47. Zhao H., Arnold F.H. Directed evolution converts subtilisin E into a functional equivalent of thermitase. Protein Engineering 1999, 12:47-53.