Knowledge-guided laboratory evolution of protein thermolability

Biotechnology and Bioengineering

Volumn 102, Issue 6, 2009, Pages 1712-1717

  Reetz, Manfred T ^a   Soni, Pankaj ^a   Fernández, Layla ^a  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

Author keywords

B factors; Directed evolution; Enzyme thermolability; Lipases; Saturation mutagenesis

Indexed keywords

B-FACTORS; CATALYTIC PROFILES; DIRECTED EVOLUTION; INCREASED FLEXIBILITIES; MODEL STUDIES; PSEUDOMONAS AERUGINOSA; ROOM TEMPERATURES; SATURATION MUTAGENESIS; WILD TYPES; X-RAY STRUCTURES;

LIPASES; MUTAGENESIS; RIGIDITY;

ENZYMES;

TRIACYLGLYCEROL LIPASE;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME KINETICS; EVOLUTION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN STABILITY; PSEUDOMONAS AERUGINOSA; THERMOSTABILITY;

BACTERIAL PROTEINS; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; GENE LIBRARY; LIPASE; MODELS, MOLECULAR; MUTAGENESIS; PSEUDOMONAS AERUGINOSA; SUBSTRATE SPECIFICITY; TEMPERATURE;

PSEUDOMONAS AERUGINOSA;

EID: 65349143121     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22202     Document Type: Article

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