Structural and thermodynamic analyses of Escherichia coli RNase HI variant with quintuple thermostabilizing mutations

FEBS Journal

Volumn 274, Issue 22, 2007, Pages 5815-5825

  Haruki, Mitsuru ^a   Tanaka, Masaki ^b   Motegi, Takehiko ^a   Tadokoro, Takashi ^b   Koga, Yuichi ^b   Takano, Kazufumi ^b   Kanaya, Shigenori ^b  

^a NIHON UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Crystal structure; Quintuple thermostabilizing mutations; RNase HI; Stability curve; Thermostability

Indexed keywords

BACTERIAL PROTEIN; RIBONUCLEASE H;

ARTICLE; CRYSTAL STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STABILITY; PROTEIN STRUCTURE; TEMPERATURE DEPENDENCE; THERMODYNAMICS; THERMOSTABILITY; THERMUS THERMOPHILUS;

CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; ESCHERICHIA COLI; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; RIBONUCLEASE H; THERMODYNAMICS;

ESCHERICHIA COLI; THERMUS THERMOPHILUS;

EID: 35748975396     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2007.06104.x     Document Type: Article

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