Quantitative evaluation of freeze-substitution effects on preservation of nuclear antigens during preparation of biological samples for immunoelectron microscopy

Histochemistry and Cell Biology

Volumn 138, Issue 1, 2012, Pages 167-177

  Sobol, Margarita A ^a   Philimonenko, Vlada V ^a   Philimonenko, Anatoly A ^a   Hozák, Pavel ^a  

^a INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

Author keywords

Automated freeze substitution; High pressure freezing; Immuno gold labeling; Immunohistochemistry; LR White

Indexed keywords

CELL NUCLEUS ANTIGEN; GLUTARALDEHYDE; ORGANIC SOLVENT;

ARTICLE; CELL STRUCTURE; EXTRACTION; FREEZE DRYING; IMMUNOELECTRON MICROSCOPY; IMMUNOGOLD LABELING; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS;

ANTIGENS, NUCLEAR; FREEZE SUBSTITUTION; GLUTARAL; HELA CELLS; HUMANS; MICROSCOPY, IMMUNOELECTRON; SOLVENTS;

EID: 84865143922     PISSN: 09486143     EISSN: 1432119X     Source Type: Journal    
DOI: 10.1007/s00418-012-0931-6     Document Type: Article

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