메뉴 건너뛰기




Volumn 130, Issue 5, 2008, Pages 1047-1052

Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR white embedding of HeLa cells

Author keywords

Cryofixation; Freeze substitution; High pressure freezing; Immunogold labelling; LR White

Indexed keywords

ACETONE; CELL NUCLEUS ANTIGEN;

EID: 54049097430     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-008-0504-x     Document Type: Article
Times cited : (9)

References (32)
  • 2
    • 0026693217 scopus 로고
    • Quantitative immuno-gold labelling and ultrastructural preservation after cryofixation (combined with different freeze-substitution and embedding protocols) and after chemical fixation and cryosectioning
    • AG Bittermann G Knoll A Nemeth H Plattner 1992 Quantitative immuno-gold labelling and ultrastructural preservation after cryofixation (combined with different freeze-substitution and embedding protocols) and after chemical fixation and cryosectioning Histochemistry 97 421 429
    • (1992) Histochemistry , vol.97 , pp. 421-429
    • Bittermann, A.G.1    Knoll, G.2    Nemeth, A.3    Plattner, H.4
  • 3
    • 0035239219 scopus 로고    scopus 로고
    • Cryosubstitution of frozen biological specimens in electron microscopy: Use and application as an alternative to chemical fixation
    • B Bohrmann E Kellenberger 2001 Cryosubstitution of frozen biological specimens in electron microscopy: use and application as an alternative to chemical fixation Micron 32 11 19
    • (2001) Micron , vol.32 , pp. 11-19
    • Bohrmann, B.1    Kellenberger, E.2
  • 4
    • 0024426168 scopus 로고
    • High-pressure freezing for the preservation of biological structure: Theory and practice
    • R Dahl LA Staehelin 1989 High-pressure freezing for the preservation of biological structure: theory and practice J Electron Microsc Tech 13 165 174
    • (1989) J Electron Microsc Tech , vol.13 , pp. 165-174
    • Dahl, R.1    Staehelin, L.A.2
  • 5
    • 0142057232 scopus 로고    scopus 로고
    • Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples
    • TH Giddings 2003 Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples J Microsc 212 53 61
    • (2003) J Microsc , vol.212 , pp. 53-61
    • Giddings, T.H.1
  • 6
    • 0142088982 scopus 로고    scopus 로고
    • Of plants and other pets: Practical aspects of freeze-substitution and resin embedding
    • MW Hess 2003 Of plants and other pets: practical aspects of freeze-substitution and resin embedding J Microsc 212 44 52
    • (2003) J Microsc , vol.212 , pp. 44-52
    • Hess, M.W.1
  • 7
    • 0027982167 scopus 로고
    • High-pressure freezing of cell suspensions in cellulose capillary tubes
    • H Hohenberg K Mannweiler M Müller 1994 High-pressure freezing of cell suspensions in cellulose capillary tubes J Microsc 175 34 43
    • (1994) J Microsc , vol.175 , pp. 34-43
    • Hohenberg, H.1    Mannweiler, K.2    Müller, M.3
  • 8
    • 0030445492 scopus 로고    scopus 로고
    • Ultrastructure of Kurloff body proteoglycans after high pressure freezing, cryosubstitution and postembedding staining with cuprolinic blue
    • G Landemore M Quillec J Izard 1996 Ultrastructure of Kurloff body proteoglycans after high pressure freezing, cryosubstitution and postembedding staining with cuprolinic blue Glycobiology 6 817 822
    • (1996) Glycobiology , vol.6 , pp. 817-822
    • Landemore, G.1    Quillec, M.2    Izard, J.3
  • 9
    • 0029120034 scopus 로고
    • Improved antigenic preservation of plant tissue by low temperature processing in LR White resin
    • DY Mashadian GW Grimes JZ Kiss 1995 Improved antigenic preservation of plant tissue by low temperature processing in LR White resin Microsc Res Tech 31 531 532
    • (1995) Microsc Res Tech , vol.31 , pp. 531-532
    • Mashadian, D.Y.1    Grimes, G.W.2    Kiss, J.Z.3
  • 10
    • 0032618726 scopus 로고    scopus 로고
    • High-pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling
    • K McDonald 1999 High-pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling Methods Mol Biol 117 77 97
    • (1999) Methods Mol Biol , vol.117 , pp. 77-97
    • McDonald, K.1
  • 11
    • 0036277570 scopus 로고    scopus 로고
    • Cryomethods for thin section electron microscopy
    • KL McDonald T Müller-Reichert 2002 Cryomethods for thin section electron microscopy Methods Enzymol 351 96 123
    • (2002) Methods Enzymol , vol.351 , pp. 96-123
    • Müller-Reichert, T.1
  • 12
    • 0025003409 scopus 로고
    • Freeze-substitution without aldehyde or osmium fixatives: Ultrastructure and implications for immunocytochemistry
    • P Monaghan D Robertson 1990 Freeze-substitution without aldehyde or osmium fixatives: ultrastructure and implications for immunocytochemistry J Microsc 158 355 363
    • (1990) J Microsc , vol.158 , pp. 355-363
    • Monaghan, P.1    Robertson, D.2
  • 13
    • 0032412495 scopus 로고    scopus 로고
    • High-pressure freezing for immunocytochemistry
    • P Monaghan N Perusinghe M Müller 1998 High-pressure freezing for immunocytochemistry J Microsc 192 248 258
    • (1998) J Microsc , vol.192 , pp. 248-258
    • Monaghan, P.1    Perusinghe, N.2    Müller, M.3
  • 14
    • 0142120569 scopus 로고    scopus 로고
    • The use of filter membranes for high-pressure freezing of cell monolayers
    • MK Morphew JR McIntosh 2003 The use of filter membranes for high-pressure freezing of cell monolayers J Microsc 212 21 25
    • (2003) J Microsc , vol.212 , pp. 21-25
    • Morphew, M.K.1
  • 15
    • 33749468527 scopus 로고    scopus 로고
    • High-pressure freezing and freeze substitution of rat myocardium for immunogold labelling of connexin 43
    • Ch Mühlfeld J Richter 2006 High-pressure freezing and freeze substitution of rat myocardium for immunogold labelling of connexin 43 Anat Rec A 288A 1059 1067
    • (2006) Anat Rec A , vol.288 , pp. 1059-1067
    • Mühlfeld, C.1    Richter, J.2
  • 16
    • 0027536126 scopus 로고
    • Freeze substitution after fast-freeze fixation in preparation for immunocytochemistry
    • MT Nicolas JM Bassot 1993 Freeze substitution after fast-freeze fixation in preparation for immunocytochemistry Microsc Res Tech 24 474 487
    • (1993) Microsc Res Tech , vol.24 , pp. 474-487
    • Nicolas, M.T.1    Bassot, J.M.2
  • 17
    • 12244289607 scopus 로고    scopus 로고
    • LR White is preferable to Unicryl for immunogold detection of fixation-sensitive nuclear antigens
    • VV Philimonenko J Janacek P Hozak 2002 LR White is preferable to Unicryl for immunogold detection of fixation-sensitive nuclear antigens Eur J Histochem 46 359 364
    • (2002) Eur J Histochem , vol.46 , pp. 359-364
    • Philimonenko, V.V.1    Janacek, J.2    Hozak, P.3
  • 18
    • 0742286842 scopus 로고    scopus 로고
    • High-pressure freezing of epithelial cells on sapphire coverslips
    • S Reipert I Fischer G Wiche 2004 High-pressure freezing of epithelial cells on sapphire coverslips J Microsc 213 81 85
    • (2004) J Microsc , vol.213 , pp. 81-85
    • Reipert, S.1    Fischer, I.2    Wiche, G.3
  • 19
    • 4043090575 scopus 로고    scopus 로고
    • High-pressure cryoimmobilization of murine skin reveals novel structural features and prevents extraction artifacts
    • S Reipert I Fischer G Wiche 2004 High-pressure cryoimmobilization of murine skin reveals novel structural features and prevents extraction artifacts Exp Dermatol 13 419 425
    • (2004) Exp Dermatol , vol.13 , pp. 419-425
    • Reipert, S.1    Fischer, I.2    Wiche, G.3
  • 20
    • 0030921318 scopus 로고    scopus 로고
    • Ultrastructural immunolocalization of polyamines in HeLa cells subjected to fast-freezing fixation and freeze substitution
    • AM Roch MT Nicolas G Quash 1997 Ultrastructural immunolocalization of polyamines in HeLa cells subjected to fast-freezing fixation and freeze substitution Histochem Cell Biol 107 303 312
    • (1997) Histochem Cell Biol , vol.107 , pp. 303-312
    • Roch, A.M.1    Nicolas, M.T.2    Quash, G.3
  • 21
    • 0024376736 scopus 로고
    • Postembedding labelling on Lowicryl K4M tissue sections: Detection and modification of cellular components
    • J Roth 1989 Postembedding labelling on Lowicryl K4M tissue sections: detection and modification of cellular components Methods Cell Biol 31 513 551
    • (1989) Methods Cell Biol , vol.31 , pp. 513-551
    • Roth, J.1
  • 22
    • 0036887012 scopus 로고    scopus 로고
    • A new approach for high-pressure freezing of primary culture cells: The fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cells
    • A Sawaguchi KL McDonald S Karvar JG Forte 2002 A new approach for high-pressure freezing of primary culture cells: the fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cells J Microsc 208 158 166
    • (2002) J Microsc , vol.208 , pp. 158-166
    • Sawaguchi, A.1    Karvar, S.2    Forte, J.G.3
  • 23
    • 0142025255 scopus 로고    scopus 로고
    • Direct attachment of cell suspensions to high-pressure freezing specimen planchettes
    • A Sawaguchi X Yao JG Forte K McDonald 2003 Direct attachment of cell suspensions to high-pressure freezing specimen planchettes J Microsc 212 13 20
    • (2003) J Microsc , vol.212 , pp. 13-20
    • Sawaguchi, A.1    Yao, X.2    Forte, J.G.3
  • 29
    • 0025986865 scopus 로고
    • Some application of cryosubstitution in ultrastructural studies of the cell nucleus
    • M-L Von Schack S Fakan W Villiger 1991 Some application of cryosubstitution in ultrastructural studies of the cell nucleus Biol Cell 72 113 119
    • (1991) Biol Cell , vol.72 , pp. 113-119
    • Von Schack, M.-L.1    Fakan, S.2    Villiger, W.3
  • 30
    • 0027345656 scopus 로고
    • Cryofixation and cryosubstitution: A useful alternative in the analyses of cellular fine structure
    • M-L Von Schack S Fakan W Villiger M Muller 1993 Cryofixation and cryosubstitution: a useful alternative in the analyses of cellular fine structure Eur J Histochem 37 5 18
    • (1993) Eur J Histochem , vol.37 , pp. 5-18
    • Von Schack, M.-L.1    Fakan, S.2    Villiger, W.3    Muller, M.4
  • 31
    • 0036440834 scopus 로고    scopus 로고
    • Freeze substitution of high-pressure frozen samples: The visibility of biological membranes is improved when the substitution medium contains water
    • P Walther A Ziegler 2002 Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water J Microsc 208 3 10
    • (2002) J Microsc , vol.208 , pp. 3-10
    • Walther, P.1    Ziegler, A.2
  • 32
    • 0035873242 scopus 로고    scopus 로고
    • Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze-substitution
    • P Wild EM Schraner H Adler BM Humbel 2001 Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze-substitution Microsc Res Tech 53 313 321
    • (2001) Microsc Res Tech , vol.53 , pp. 313-321
    • Wild, P.1    Schraner, E.M.2    Adler, H.3    Humbel, B.M.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.