High-pressure freezing and freeze-substitution of native rat brain: Suitability for preservation and immunoelectron microscopic localization of myelin glycolipids

Journal of Neuroscience Research

Volumn 53, Issue 4, 1998, Pages 465-474

  Kirschning, Eckhard ^a,b   Rutter, Gabriel ^a   Hohenberg, Heinz ^a  

^a UNIVERSITY OF HAMBURG   (Germany)

^b LEIBNIZ INSTITUTE FOR EXPERIMENTAL VIROLOGY   (Germany)

Author keywords

Galactocerebroside; High pressure cryofixation; Native brain tissue; Postembedding immunogold labeling; Sulfatide

Indexed keywords

EPITOPE; GALACTOSYLCERAMIDE; GLYCOLIPID; MONOCLONAL ANTIBODY; MYELIN; ORGANIC SOLVENT; SULFATIDE;

ANIMAL TISSUE; ANTIGEN RECOGNITION; ARTICLE; BRAIN; BRAIN CORTEX; CELL ULTRASTRUCTURE; CONTROLLED STUDY; EMBEDDING; FREEZE DRYING; FREEZING; IMMUNOELECTRON MICROSCOPY; IMMUNOGOLD STAINING; NERVE CELL DIFFERENTIATION; NONHUMAN; OLIGODENDROGLIA; ORGAN PRESERVATION; PRIORITY JOURNAL; RAT; TISSUE DISTRIBUTION; TISSUE FIXATION;

ANIMALS; BIOPSY, NEEDLE; BRAIN; CRYOPRESERVATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPOXY RESINS; FREEZE SUBSTITUTION; GLYCOLIPIDS; MICROSCOPY, ELECTRON; MICROSCOPY, IMMUNOELECTRON; MYELIN SHEATH; PRESSURE; RATS;

EID: 0031879716     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4547(19980815)53:4<465::AID-JNR8>3.0.CO;2-4     Document Type: Article

References (51)

1. Abe T, Norton WT (1974): The characterization of sphingolipids from neurons and astroglia of immature rat brain. J Neurochem 23:1025-1036.
2. Bansal R, Pfeiffer SE (1992): Novel stage in the oligodendrocyte lineage defined by reactivity of progenitors with R-mAb prior to O1 anti-galactocerebroside. J Neurosci Res 32:309-316.
3. Bansal R, Warrington AE, Card AL, Ranscht B, Pfeiffer SE (1989): Multiple and novel specificities of monoclonal antibodies O1, O4 and R-mAb used in the analysis of oligodendrocyte development. J Neurosci Res 24:548-557.
4. Bansal R, Stefansson K, Pfeiffer SE (1992): Proligodendroblast antigen (POA), a developmental antigen expressed by A007/O4-positive oligodendrocyte progenitors prior to the appearance of sulfatide and galactocerebroside. J Neurochem 58:2221-2229.
5. Calderon RO, Attema B, DeVries GH (1995): Lipid composition of neuronal cell bodies and neurites from cultured dorsal root ganglia. J Neurochem 64:424-429.
6. Chandler DE, Heuser J (1979): Comparison of quick-frozen and chemically fixed sea urchin eggs: Structural evidence that cortical granule exocytosis is preceded by a local increase in membrane mobility. J Cell Biol 83:91-108.
7. De Mey J, Moeremans M (1986): The preparation of colloidal gold probes and their use as marker in electron microscopy. In Koehler JK (ed): "Advanced Techniques in Biological Electron Microscopy III." Berlin: Springer-Verlag, pp 229-271.
8. Dubochet J (1995): High pressure freezing for cryoelectron microscopy. Trends Cell Biol 5:366-368.
9. Dubois-Dalcq M (1987): Characterization of a slowly proliferative cell along the oligodendrocyte differentiation pathway. EMBO J 6:2587-2595.
10. Ghandour MS, Skoff RP (1988): Expression of galactocerebroside in developing normal and jimpy oligodendrocytes in situ. J Neurocytol 17:485-498.
11. Gocht A, Löhler J (1993): Microenvironmental changes during axonal regrowth in the optic nerve of the myelin deficient rat. J Neurocytol 22:461-479.
12. Guerci A, Monge M, Baron-Van Evercooren A, Lubetzki C, Dancea S, Boutry JM, Goujet-Zalc C, Zalc B (1986): Schwann cell marker defined by a monoclonal antibody (224-58) with species cross-reactivity. I. Cellular localization. J Neurochem 46:425-434.
13. Hanyu Y, Ichikawa M, Matsumoto G (1992): An improved cryofixation method: Cryoquenching of small tissue blocks during microwave irradiation. J Microsc 165:255-278.
14. Hara A, Taketomi T, Iwata M, Ando M, Nagata Y (1988): Differences in neuronal lipid composition between superior cervical ganglia and nodose ganglia of the rat. Biochim Biophys Acta 960:427-434.
15. Hardy RJ, Friedrich VL (1996): Progressive remodeling of the oligodendrocyte process arbor during myelinogenesis. Dev Neurosci 18:243-254.
16. Hayat MA (1981): "Fixation for Electron Microscopy." New York: Academic Press.
17. Hohenberg H, Mannweiler K, Müller M (1994): High-pressure freezing of cell suspensions in cellulose capillary tubes. J Microsc 175:34-43.
18. Hohenberg H, Tobler M, Müller M (1996): High-pressure freezing of tissue obtained by fine-needle biopsy. J Microsc 183:133-139.
19. Kirschning E, Rutter G, Huckhagel C, Ellhof I, Hohenberg H (1997): A sulfatide-reactive monoclonal antibody derived from a patient with multiple sclerosis binds to myelin in situ. Ann NY Acad Sci 815:455-458.
20. LeVine SM, Goldman JE (1988): Embryonic divergence of oligodendrocyte and astrocyte lineages in developing rat cerebrum. J Neurosci 8:3992-4006.
21. Li J, Pearl DK, Pfeiffer SE, Yates AJ (1994): Pattern of reactivity with anti-glycolipid antibodies in human primary brain tumors. J Neurosci Res 39:148-158.
22. Luft JH (1961): Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409-414.
23. Mirsky R, Winter J, Abney E, Pruss ER, Gavrilovic J, Raff MC (1980): Myelin-specific proteins and galactolipids in rat Schwann cells and oligodendrocytes in culture. J Cell Biol 84:483-494.
24. Moor M (1987): Theory and practice of high pressure freezing. In Steinbrecht RA, Zierold K (eds): "Cryotechniques in Biological Electron Microscopy." Heidelberg: Springer Verlag, pp 175-191.
25. Morgan TE, Huber GL (1967): Loss of lipid during fixation for electron microscopy. J Cell Biol 32:757-760.
26. Müller M, Marti T, Kriz S (1980): Improved structural preservation by freeze substitution. In Brederoo P, de Priester W (eds): Proceedings of the 7th European Congress on Electron Microscopy pp 720-721.
27. Nardelli E, Anzini P, Moretto G, Rizzuto N, Steck AJ (1994): Pattern of nervous-tissue immunostaining by human anti-glycolipid antibodies. J Neuro Sci 122:220-227.
28. Nonaka G, Kishimoto Y (1979): Levels of cerebrosides, sulfatides and galactosyl diglycerides in different regions of rat brain. Biochim Biophys Acta 572:432-441.
29. Norton WT (1977): Isolation and characterization of myelin. In Morell P (ed): "Myelin." New York: Plenum Press, pp 161-169.
30. Prineas JW, Kwon EE, Goldenberg PZ, Ilyas AA, Quarles RH, Benjamins JA, Sprinkle TJ (1989): Multiple sclerosis: Oligodendrocyte proliferation and differentiation in fresh lesions. Lab Invest 61:489-503.
31. Raff MC, Mirsky R, Fields KL, Lisak RP, Dorfmann SH, Silberberg DH, Gregson NA, Liebowitz S, Kennedy M (1978): Galactocerebroside: A specific cell surface antigenic marker for oligodendrocytes in culture. Nature 274:813-816.
32. Ranscht B, Clapshaw PA, Noble M, Seifert W (1982): Development of oligodendrocytes and Schwann cells studied with a monoclonal antibody against galactocerebroside. Proc Natl Acad Sci USA 79:2709-2713.
33. Rees RP, Reese TS (1981): New structural features of freeze-substituted neuritic growth corns. Neurosci 6:247-254.
34. Reynolds EW (1963): The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208-212.
35. Reynolds R, Hardy R (1997): Oligodendroglial progenitors labeled with the O4 antibody persist in the adult rat cerebral cortex in vivo. J Neurosci Res 47:455-470.
36. Reynolds R, Wilkin GP (1988): Development of macroglial cells in rat cerebellum. II. An in situ immunohistochemical study of Oligodendroglial lineage from precursor to mature myelinating cell. Development 102:409-425.
37. Rutter G, Hohenberg H (1991): Simultaneous demonstration of antigens on outer and protoplasmic surfaces by replica immunochemistry. In Hayat MA (ed): "Colloidal Gold: Principles, Methods and Applications." Vol. 3. New York: Academic Press, pp 151-186.
38. Sakakibara K, Momoi T, Uchida T, Nagai Y (1981): Evidence for association of glycosphingolipid with a colchicine-sensitive microtubule-like cytoskeletal structure in cultured cells. Nature 293:76-79.
39. Sartori N, Richter K, Dubochet J (1993): Vitrification depth can be increased more than 10 fold by high pressure freezing. J Microsc 172:49-54.
40. Schiff R, Rosenbluth J (1995): Distribution of myelin lipid antigens in adult and developing rat spinal cord. Brain Res 686:143-149.
41. Sommer I, Schachner M (1981): Monoclonal antibodies (O1 to O4) to oligodendrocyte cell surfaces: an immunocytological study in the central nervous system. Dev Biol 83:311-327.
42. Sparkman DR, White CL (1989): A simple apparatus for processing large numbers of specimens for colloidal gold immunoelectron microscopy: Application to paired helical filaments of Alzheimer's disease. J Electron Microsc Techn 13:152-153.
43. Studer D, Michel M, Müller M (1989): High pressure freezing comes of age. Scanning Microsc 3:253-269.
44. Uhlig H, Dernick R (1989): Monoclonal autoantibodies derived from multiple sclerosis patients and control persons and their reactivities with antigens of the central nervous system. Autoimmunity 5:87-99.
45. van Genderen IL, van Meer G, Slot JW, Geuze HJ, Voorhout WF (1991): Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electronmicroscopy after freeze-substitution. J Cell Biol 115:1009-1019.
46. Van Harreveld A, Crowell J (1964): Electron microscopy after rapid freezing on a metal surface and substitution fixation. Anat Rec 149:381-386.
47. Ward BJ, Gloster JA (1976): Lipid losses during processing of cardiac muscle for electron microscopy. J Microsc 108:41-50.
48. Warrington AE, PfeifferSE (1992): Proliferation and differentiation of 04+ oligodendrocytes in postnatal rat cerebellum: Analysis in unfixed tissue slices using anti-glycolipid antibodies. J Neurosci Res 33:338-353.
49. Weibull C, Christiansson A (1986): Extraction of proteins and membrane lipids during low temperature embedding of biological material for electron microscopy. J Microsc 142:79-86.
50. Weibull C, Christiansson A, Carlemalm E (1983): Extraction of membrane lipids during fixation, dehydration and embedding of Acholeplasma laidlawii-cells for electron microscopy. J Microsc 129:201-207.
51. Zalcq B, Monge M, Dupouey P, Hauw JJ, Baumann NA (1981): Immunohistochemical localization of galactosyl- and sulfogalactosylceramide in the brain of 30-day-old mouse. Brain Res 211:41-354.