Immunogold labeling of cryosections from high-pressure frozen cells

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Volumn 8, Issue 5, 2007, Pages 471-485

  van Donselaar, Elly ^a   Posthuma, George ^a   Zeuschner, Dagmar ^a,b   Humbel, Bruno M ^c   Slot, Jan W ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^b MAX PLANCK INSTITUTE FOR MOLECULAR BIOMEDICINE   (Germany)

^c UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Cryosubstitution fixation; High pressure freezing; Immunocytochemistry; Rehydration; Section fixation; Tokuyasu cryosectioning

Indexed keywords

FORMALDEHYDE; GLUTARALDEHYDE; METHYLCELLULOSE; OSMIUM TETRAOXIDE; SUCROSE; URANYL ACETATE; WATER;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CARTILAGE; CARTILAGE CELL; CELL STRAIN HEPG2; CELL STRUCTURE; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; CULTURE TECHNIQUE; FREEZING; HUMAN; HUMAN CELL; IMMUNOELECTRON MICROSCOPY; IMMUNOGOLD LABELING; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; PANCREAS; PRIORITY JOURNAL; REHYDRATION; ROOM TEMPERATURE; THAWING;

AMYLASES; ANIMALS; ANTIBODIES; CARTILAGE; CELL LINE, TUMOR; CELLS, CULTURED; CHONDROCYTES; CRYOPRESERVATION; CRYOULTRAMICROTOMY; FORMALDEHYDE; GLUTARAL; HUMANS; IMMUNOHISTOCHEMISTRY; MICE; MICROSCOPY, IMMUNOELECTRON; ORGANELLES; ORGANOMETALLIC COMPOUNDS; OSMIUM TETROXIDE; PANCREAS, EXOCRINE; RATS; RATS, WISTAR; STAINING AND LABELING; SUPEROXIDE DISMUTASE; TISSUE FIXATION; VESICULAR TRANSPORT PROTEINS;

EID: 34247323554     PISSN: 13989219     EISSN: 16000854     Source Type: Journal    
DOI: 10.1111/j.1600-0854.2007.00552.x     Document Type: Article

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