Electron microscopy of the early Caenorhabditis elegans embryo

Journal of Microscopy

Volumn 230, Issue 2, 2008, Pages 297-307

  Muller Reichert T ^a   Mantler J ^a   Srayko, M ^b   O'Toole, E ^c  

^a MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

^b UNIVERSITY OF ALBERTA   (Canada)

^c UNIVERSITY OF COLORADO   (United States)

Author keywords

3D reconstruction; Caenorhabditis elegans; Electron microscopy; Electron tomography; Freeze substitution; High pressure freezing; Meiosis; Mitosis

Indexed keywords

ELECTRIC IMPEDANCE TOMOGRAPHY; ELECTRON MICROSCOPES; ELECTRONS; FREEZING; IMAGE RECONSTRUCTION;

3D RECONSTRUCTION; CAENORHABDITIS ELEGANS; CELLULARS; ELECTRON TOMOGRAPHY; FREEZE SUBSTITUTION; HIGH-PRESSURE FREEZING; MEIOSIS; MITOSIS; MODELLING SYSTEMS; TIME-RESOLVED;

ELECTRON MICROSCOPY;

CAENORHABDITIS ELEGANS; CONFERENCE PAPER; CONTROLLED STUDY; DEVELOPMENTAL STAGE; ELECTRON MICROSCOPY; EMBRYO DEVELOPMENT; FREEZE DRYING; NONHUMAN; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING; ULTRASTRUCTURE;

CAENORHABDITIS ELEGANS;

EID: 42649136908     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2008.01985.x     Document Type: Conference Paper

References (55)

1. Albertson, D.G. (1984) Formation of the first cleavage spindle in nematode embryos. Dev. Biol. 101, 61 72.
2. Albertson, D.G. & Thomson, J.N. (1982) The kinetochores of Caenorhabditis elegans. Chromosoma 86, 409 428.
3. Albertson, D.G. & Thomson, J.N. (1993) Segregation of holocentric chromosomes at meiosis in the nematode, Caenorhabditis elegans. Chromosome Res. 1, 15 26.
4. Bullitt, E., Rout, M.P., Kilmartin, J.V. & Akey, C.W. (1997) The yeast spindle pole body is assembled around a central crystal of Spc42p. Cell 89, 1077 1086.
5. Dahl, R. & Staehelin, L.A. (1989) High pressure freezing for the preservation of biological structure: theory and practice. J. Electron Microsc. Techn. 13, 165 174.
6. Dammermann, A., Müller-Reichert, T., Pelletier, L., Habermann, B., Desai, A. & Oegema, K. (2004) Centriole assembly requires both centriolar and pericentriolar material proteins. Dev. Cell 7, 815 829.
7. Dong, Y., Vanden Beldt, K.L., Meng, X., Khodjakov, A. & McEwen, B.F. (2007) The outer plate in vertebrate kinetochores is a flexible network with multiple microtubule interactions. Nat. Cell Biol. 9, 516 522.
8. Frank, J., Wagenknecht, T., McEwen, B.F., Marko, M., Hsieh, C. & Mannella, C.A. (2002) Three-dimensional imaging of biological complexity. J. Struct. Biol. 138, 85 91.
9. Gilbert, P.F.C. (1972) The reconstruction of a three-dimensional structure from projections and its application to electron microscopy. II. Direct methods. Proc. R. Soc. Lond. B Biol. Sci. 182, 89 102.
10. Harris, J.E., Govindan, J.A., Yamamoto, I., Schwartz, J., Kaverina, I. & Greenstein, D. (2006) Major sperm protein signaling promotes oocyte microtubule reorganization prior to fertilization in Caenorhabditis elegans. Dev. Biol. 299, 105 121.
11. Hohenberg, H., Mannweiler, K. & Sleytr, U.B. (1994) High pressure freezing of cell suspensions in cellulose capillary tubes. J. Microsc. 175, 34 43.
12. Höög, J.L., Schwartz, C., Noon, A.T., O'Toole, E.T., Mastronarde, D.N., McIntosh, J.R. & Antony, C. (2007) Organization of interphase microtubules in fission yeast analyzed by electron tomography. Dev. Cell 12, 349 361.
13. Howe, M., McDonald, K.L., Albertson, D.G. & Meyer, B.J. (2001) HIM-10 is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes. J. Cell Biol. 153, 1227 1238.
14. Kirkham, M., Müller-Reichert, T., Oegema, K., Grill, S. & Hyman, A.A. (2003) SAS-4 is a C. elegans centriolar protein that controls centrosome size. Cell 112, 575 587.
15. Kremer, J.R., Mastronarde, D.N. & McIntosh, J.R. (1996) Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116, 71 76.
16. Ladinsky, M.S., Mastronarde, D.N., McIntosh, J.R., Howell, K.E. & Staehelin, L.A. (1999) Golgi structure in 3 dimensions: functional insights from the NRK cell. J. Cell Biol. 144, 1135 1149.
17. Manninen, A., Verkade, P., Le Lay, S., Torkko, J., Kasper, M., Füllekrug, J., & Simons, K. (2005) Caveolin-1 is not essential for biosynthetic apical membrane transport. Mol. Cell. Biol. 25, 10087 10096.
18. Marsh, B.J., Mastronarde, D.N., Buttle, K.F., Howell, K.E. & McIntosh, J.R. (2001) Organellar relationships in the Golgi region of the pancreatic beta cell line, HIT-T15, visualized by high resolution electron tomography. Proc. Natl. Acad. Sci. U S A 98, 2399 2406.
19. Mastronarde, D.N. (1997) Dual-axis tomography: an approach with alignment methods that preserve resolution. J. Struct. Biol. 120, 343 352.
20. McDonald, K.L. (1994) Electron microscopy and EM immunohistochemistry. Methods Cell Biol. 44, 411 444.
21. McDonald, K. (2007) Cryopreparation methods for electron microscopy of selected model systems. Methods Cell Biol. 79, 23 56.
22. McDonald, K. & Müller-Reichert, T. (2002) Cryomethods for thin section electron microscopy. Methods Enzymol. 351, 96 123.
23. McDonald, K.L., Morphew, M., Verkade, P. & Müller-Reichert, T. (2007) Recent advances in high-pressure freezing. Methods Mol. Biol. 369, 143 173.
24. McEwen, B.F. & Marko, M. (2001) The emergence of electron tomography as an important tool for investigating cellular ultrastructure. J. Histochem. Cytochem. 49, 553 563.
25. McIntosh, R., Nicastro, D. & Mastronarde, D. (2005) New views of cells in 3D: an introduction to electron tomography. Trends Cell Biol. 15, 43 51.
26. Moritz, M., Braunfeld, M.B., Fung, J.C., Sedat, J.W., Alberts, B.M. & Agard, D.A. (1995) Three-dimensional structural characterization of centrosomes from early Drosophila embryos. J. Cell Biol. 130, 1149 1159.
27. Müller-Reichert, T., Hohenberg, H., O'Toole, E.T. & McDonald, K. (2003) Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure. J. Microsc. 212, 71 80.
28. Müller-Reichert, T., Srayko, M., Hyman, A.A., O'Toole, E. & McDonald, K. (2007) Correlative light and electron microscopy of early Caenorhabditis elegans embryos in mitosis. Method Cell Biol. 79, 101 119.
29. Murk, J.L., Posthuma, G., Koster, A.J., Geuze, H.J., Verkleij, A.J., Kleijmeer, M.J. & Humbel, B. (2003) Influence of aldehyde fixation on the morphology of endosomes and lysosomes: quantitative analysis and electron tomography. J. Microsc. 212, 81 90.
30. O'Connell, K.F., Caron, C., Kopish, K.R., Hurd, D.D., Kemphues, K.J., Li, Y. & White, J.G. (2001) The C. elegans zyg-1 gene encodes a regulator of centrosome duplication with distinct maternal and paternal roles in the embryo. Cell 105, 547 558.
31. O'Toole, E.T., Winey, M. & McIntosh, J.R. (1999) High-voltage electron tomography of spindle pole bodies and early mitotic spindles in the yeast Saccharomyces cerevisiae. Mol. Biol. Cell. 10, 2017 2031.
32. O'Toole, E.T., Giddings, T.H., McIntosh, J.R. & Dutcher, S.K. (2003a) Three-dimensional organization of basal bodies from wild-type and δ-tubulin deletion strains of Chlamydomonas reinhardtii. Mol. Biol. Cell. 14, 2999 3012.
33. O'Toole, E.T., McDonald, K.L., Mäntler, J., McIntosh, J.R., Hyman, A.A. & Müller-Reichert, T. (2003b) Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans. J. Cell Biol. 163, 451 456.
34. Oegema, K. & Hyman, A.A. Cell division (2006 WormBook (ed. The C. elegans Research Community, Wormbook) doi/10.1895/wormbook.172.1, http://www.wormbook.org.
35. Oegema, K., Desai, A., Rybina, S., Kirkham, M. & Hyman, A.A. (2001) Functional analysis of kinetochore assembly in Caenorhabditis elegans. J. Cell Biol. 153, 1209 1226.
36. Özlü, N., Srayko, M., Kinoshita, K. et al. (2005) An essential function of the C. elegans ortholog of TPX2 is to localize activated aurora A kinase to mitotic spindles. Dev. Cell 9, 237 248.
37. Pelletier, L., Müller-Reichert, T., Srayko, M., Özlü, N., Schlaitz, A.-L. & Hyman, A.A. (2004a) The C. elegans centrosome during early embryonic development. Centrosomes in Development and Disease (ed. by E. Nigg pp. 227 250. Wiley-VCH, Weinheim.
38. Pelletier, L., Özlü, N., Hannak, E. et al. (2004b) The Caenorhabditis elegans centrosomal protein SPD-2 is required for both pericentriolar material recruitment and centriole duplication. Curr. Biol. 14, 863 873.
39. Pelletier, L., O'Toole, E., Schwager, A., Hyman, A.A. & Müller-Reichert, T. (2006) Centriole assembly in Caenorhabditis elegans. Nature 444, 619 623.
40. Priess, J.R. & Hirsh, D.I. (1986) Caenorhabditis elegans morphogenesis: the role of the cytoskeleton in elongation of the embryo. Dev. Biol. 117, 156 173.
41. Schlaitz, A.-L., Srayko, M., Dammermann, A. et al. (2007) The C. elegans RSA complex localizes protein phosphatase 2A to centrosomes and regulates mitotic spindle assembly. Cell 128, 115 127.
42. Schnackenberg, B.J., Khodjakov, A., Rieder, C.L. & Palazzo, R.E. (1998) The disassembly and reassembly of functional centrosomes in vitro. Proc. Natl. Acad. Sci. U S A 95, 9295 9300.
43. Shepard, B. & Clark, C. (1976) Structure of the anterior alimentary tract of the passively feeding nematode: Pratylenchus penetrans (Nematoda: Pratylenchidae). Nematologica 31, 321 334.
44. Sims, P.A. & Hardin, J.D. (2007) Fluorescence-integrated transmission electron microscopy images. Methods Mol. Biol. 369, 291 308.
45. Sönnichsen, B., Koski, L.B. et al. (2005) Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans. Nature 434, 462 469.
46. Srayko, M., Kaya, A., Stamford, J. & Hyman, A.A. (2005) Identification and characterization of factors required for microtubule growth and nucleation in the early C. elegans embryo. Dev. Cell 9, 223 236.
47. Srayko, M., O'Toole, E.T., Hyman, A.A. & Müller-Reichert, T. (2006) Katanin disrupts the microtubule lattice and increases polymer number in C. elegans meiosis. Curr. Biol. 16, 1944 1949.
48. Studer, D., Michel, M. & Müller, M. (1989) High-pressure freezing comes of age. Scann. Microsc. 3(Suppl), 253 269.
49. Sulston, J.E., Schierenberg, E., White, J.G. & Thomson, J.N. (1983) The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev. Biol. 100, 64 119.
50. Vancoppenolle, B., Claeys, M., Borgonie, G., Tytgat, T. & Coomans, A. (2000) Evaluation of fixation methods for ultrastructural study of Caenorhabditis elegans embryos. Microsc. Res. Tech. 49, 212 216.
51. VandenBeldt, K.J., Barnard, R.M., Hergert, P.J., Meng, X., Maiato, H. & McEwen, B.F. (2006) Kinetochores use a novel mechanism for coordinating the dynamics of individual microtubules. Curr. Biol. 16, 1217 1223.
52. Verkade, P. (2008) Moving EM: the rapid transfer system as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing. J. Microsc. 230, 317 328.
53. Weimer, R.M. (2006) Preservation of C. elegans tissue via high-pressure freezing and freeze-substitution for ultrastructural analysis and immunocytochemistry. Methods Mol. Biol. 351, 203 221.
54. Wood, W.B. (1988) The Nematode Caenorhabditis elegans. Cold Spring Harbour Laboratory.
55. Yoder, T.J., McElwain, M.A., Francis, S.E. et al. (2005) Analysis of a spindle pole body mutant reveals a defect in biorientation and illuminates spindle forces. Mol. Biol. Cell. 16, 141 152.