Freeze substitution of high-pressure frozen samples: The visibility of biological membranes is improved when the substitution medium contains water

Journal of Microscopy

Volumn 208, Issue 1, 2002, Pages 3-10

  Walther, Paul ^a   Ziegler, A ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

Cryo preparation; Electron microscopy; Freeze substitution; High pressure freezing; Membranes; TEM; Yeast

Indexed keywords

BIOLOGICAL MEMBRANES; FREEZING; HIGH PRESSURE EFFECTS; MAMMALS; TISSUE; VISIBILITY; YEAST;

CRYO-PREPARATION; FREEZE SUBSTITUTION; FROZEN SAMPLE; HIGH PRESSURE; HIGH-PRESSURE FREEZING; ONE-FACTOR; TEM; YEAST CELL;

CYTOLOGY;

WATER;

ANIMAL TISSUE; ARTICLE; CULTURE MEDIUM; FREEZE DRYING; HYPERBARISM; MEMBRANE; NONHUMAN; PANCREAS; PRIORITY JOURNAL; RAT; SAMPLE; VISIBILITY; WATER CONTENT; YEAST CELL;

ANIMALIA; ARTHROPODA;

EID: 0036440834     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.2002.01064.x     Document Type: Article

References (16)

1. Ding, R., McDonald, K.L. & McIntosh, J.R. (1993) Three-dimensional reconstruction and analysis of mitotic spindles from the yeast Schizzosaccharomyces pombe. J. Cell. Biol. 120, 141-151.
2. Erk, I., Nicolas, G., Caroff, A. & Lepault, J. (1998) Electron microscopy of frozen biological objects: A study using cryosectioning and cryosubstitution. J. Microsc. 189, 236-248.
3. Hohenberg, H., Mannweiler, K. & Müller, M. (1994) High-pressure freezing of cell suspensions in cellulose capillary tubes. J. Microsc. 175, 34-43.
4. Humbel, B. & Müller, M. (1985) Freeze substitution and low temperature embedding. Science of Biological Specimen Preparation (ed. by M. Mueller, R. P. Becker, A. Boyde and J. J. Wolosewick), pp. 195-201. SEM Inc., AMF O'Hare, Chicago.
5. Moor, H. & Riehle, U. (1968) Snap-freezing under high pressure: A new fixation technique for freeze-etching. Proceedingss 4th European Reg. Conf. Electron Microsc. 2, 33-34.
6. Müller, M., Marti, Th & Kriz, S. (1980) Improved structural preservation by freeze-substitution. Proceedings 7th Europ. Congr. on Electron Microscopy, Hague. 2, 720-721.
7. Müller, M. & Moor, H. (1984) Cryo-fixation of suspensions and tissues by propane jet freezing and high-pressure freezing. Proceedings of the 42nd Ann. Meet. Electron Microsc. Soc. Am. (ed. by G. W. Bailey), pp. 6-9. San Francisco Press, San Francisco.
8. Shimoni, E. & Müller, M. (1998) On optimizing high-pressure freezing: From heat transfer theory to a new microbiopsy device. J. Microsc. 192, 236-247.
9. Studer, D., Michel, M., Hunziker, E.B. & Buschmann, M. (1994) A new high-pressure freezing apparatus: The importance of improving cooling rates during high-pressure freezing Proceedings of the 52nd Annual Meeting of the Microscopy Society of America (ed. by G. W. Bailey and A. J. Garrat-Reed), pp. 138-139. San Francisco Press, Inc, San Francisco.
10. Studer, D., Michel, M. & Müller, M. (1989) High-pressure freezing comes of age. Scanning Microsc. Supplement, 3, 253-269.
11. Studer, D., Michel, M., Wohlwend, M., Hunziker, E.B. & Buschmann, M.D. (1995) Vitrification of articular cartilage by high-pressure freezing. J. Microsc. 179, 321-332.
12. Szczesny, P.J., Walther, P. & Müller, M. (1996) Light damage in rod outer segments: The effects of fixation on ultrastructural alterations. Current Eye Res. 15, 807-814.
13. Walther, P. & Müller, M. (1999) Biological ultrastructure as revealed by high resolution cryo-SEM of blockfaces after cryo-sectioning. J. Microsc. 196, 279-287.
14. Walther, P., Müller, M. & Schweingruber, M.E. (1984) The ultrastructure of the cell surface and plasma membrane of exponential and stationary phase cells of Schizzosaccharomyces pombe, grown in different media. Arch. Microbiol. 137, 128-134.
15. Wild, P., Schraner, E.M., Adler, H. & Humbel, B.M. (2001) Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze-substitution. Microsc. Res. Techn. 53, 313-321.
16. Ziegler, A. (1997) Ultrastructural changes of the anterior and posterior sternal integument of the terrestrial isopod Porcellio scaber Latr. (Crustacea) during the moult cycle. Tissue Cell, 29, 63-76.