High-pressure freezing of tissue obtained by fine-needle biopsy

Journal of Microscopy

Volumn 183, Issue 2, 1996, Pages 133-139

  Hohenberg, H ^a,c   Tobler, M ^b   Muller M ^a  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY HOSPITAL ZURICH   (Switzerland)

^c UNIVERSITY OF HAMBURG   (Germany)

Author keywords

Fine needle biopsy; Freeze substitution; High pressure freezing; Rat heart; Rat liver

Indexed keywords

BIOPSY; FREEZING; NEEDLES; RATS;

BIOLOGICAL TISSUES; FINE NEEDLE BIOPSY; FREEZE SUBSTITUTION; HIGH-PRESSURE FREEZING; ICE CRYSTALS; OPTIMAL THICKNESS; RAT HEART; RAT LIVERS; STRUCTURAL ALTERATIONS; TISSUE DAMAGE;

TISSUE;

ANIMAL TISSUE; ARTICLE; ELECTRON MICROSCOPY; FREEZING; HEART; LIVER; NEEDLE BIOPSY; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALIA;

EID: 0029845359     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1996.820642.x     Document Type: Article

References (16)

1. Eppenberger-Eberhardt, M., Riesinger, I., Messerli, M., Schwarb, P., Müller, M., Eppenberger, H.M. & Wallimann, T. (1991) Adult rat cardiomyocytes cultured in creatine-deficient medium display large mitochondria with paracrystalline inclusions, enriched for creatine kinase. J. Cell Biol. 113, 289-302.
2. Hanyu, Y., Ichikawa, M. & Matsumoto, G. (1992) An improved cryofixation method: cryoquenching of small tissue blocks during microwave irradiation. J. Microsc. 165, 255-278.
3. Heuser, J.E., Reese, T.S., Dennis, M.J., Jan, J., Jan, L. & Evans, L. (1979) Synaptic vesicles exocytosis captured by quick freezing and correlated with quantal transmitter release. J. Cell Biol. 81, 275-300.
4. Hohenberg, H., Mannweiler, K. & Müller, M. (1994) High pressure freezing of cell suspensions in cellulose capillary tubes. J. Microsc. 175, 34-43.
5. Knoll, G., Braun, C. & Plattner, H. (1991) Quenched flow analysis of exocytosis in Paramecium cells: time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells. J. Cell Biol. 113, 1295-1304.
6. Luft, J.H. (1961) Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 9, 409-414.
7. Otto, Ch.R. & Wellauer, J. (1985) Ultraschallgeführte Biopsie. Springer-Verlag, Heidelberg.
8. Reynolds, E.W. (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208-212.
9. Sartori, N., Richter, K. & Dubochet, J. (1993) Vitrification depth can be increased more than 10-fold by high-pressure freezing. J. Microsc. 172, 55-61.
10. Sitte, H., Edelmann, L. & Neumann, K. (1987) Cryofixation without pre treatment at ambient pressure. Cryotechniques in Biological Electron Microscopy (ed. by R. A. Stinbrecht & K. Zierold), pp. 87-114. Springer-Verlag, Berlin.
11. Studer, D., Michel, M. & Müller, M. (1989) High pressure freezing comes of age. Scanning Microsc. 3, 253-269.
12. Tobler, M., Zierold, K., Ammann, R.W. & Freiburghaus, A.U. (1992) Element distribution in organelles of pancreatic acinar cells of rat, mouse, and pig investigated by energy-dispersive X-ray microanalysis. Pancreas, 7, 686-697.
13. Willemer, S., Rüschoff, J., Adler, G., Schwerk, W.B. & Arnold, R. (1990) Ultrastrukturelle Diagnostik neuroendokriner Tumoren an Feinnadelpunktaten. Z. Gastroenterol. 28, 565-570.
14. von Zglinicki, T. (1991) Reliability of intracellular water and ion distribution as measured by X-ray microanalysis - a review. Scanning Microsc., Suppl. 5, S85-S93.
15. Zierold, K. (1993) The cryopuncher: a pneumatic cryofixation device for X-ray microanalysis of tissue specimens. J. Microsc. 171, 267-272.
16. Zschoch, H., Dominok, G.W. & Justus, J. (1991) Biopsiedagnostik. Acta Histochemica. Supplementband 39, Gustav Fischer Verlag, Jena.