A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin

Histochemistry and Cell Biology

Volumn 135, Issue 1, 2011, Pages 103-110

  Sobol, Margarita ^a   Nebesářová, Jana ^b   Hozák, Pavel ^a  

^a INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

^b INSTITUTE OF PARASITOLOGY   (Czech Republic)

Author keywords

Automated freeze substitution; High pressure freezing; Histochemistry; LR White; Mitotic cells; Ultrastructure

Indexed keywords

RESIN;

ANIMAL CELL; ARTICLE; EMBEDDING; IMMUNOCYTOCHEMISTRY; IMMUNOGOLD LABELING; LOW TEMPERATURE; MITOSIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROCESSING; ULTRASTRUCTURE;

CRYOPRESERVATION; HELA CELLS; HISTOLOGICAL TECHNIQUES; HUMANS; IMMUNOHISTOCHEMISTRY; INTERPHASE; MITOSIS;

EID: 78651496170     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-010-0771-1     Document Type: Article

References (66)

1. JD Acetarin E Carlemalm W Villiger 1986 Developments of new Lowicryl resins for embedding biological specimens at even lower temperatures J Microsc 143 81 88 1:STN:280:DyaL2s%2FhtFKrsQ%3D%3D 3531521
2. AG Bittermann G Knoll A Nemeth H Plattner 1992 Quantitative immuno-gold labelling and ultrastructural preservation after cryofixation (combined with different freeze-substitution and embedding protocols) and after chemical fixation and cryosectioning. Analysis of the secretory organelle matrix of Paramecium trichocysts Histochemistry 97 421 429 1:CAS:528:DyaK38XltVOksrw%3D 10.1007/BF00270389 1500298
3. B Bohrmann E Kellenberger 2001 Cryosubstitution of frozen biological specimens in electron microscopy: use and application as an alternative to chemical fixation Micron 32 11 19 1:STN:280:DC%2BD3M%2FhvFOmsw%3D%3D 10.1016/S0968-4328(00)00029-9 10900376
4. T Bowes B Singh RS Gupta 2007 Subcellular localization of fumarase in mammalian cells and tissues Histochem Cell Biol 127 335 346 1:CAS:528: DC%2BD2sXhsFCgsrw%3D 10.1007/s00418-006-0249-3 17111171
5. C Buser P Walther 2008 Freeze-substitution: the addition of water to polar solvents enhances the retention of structure and acts at temperatures around -60°C J Microsc 230 268 277 1:CAS:528:DC%2BD1cXnt12qt74%3D 10.1111/j.1365-2818.2008.01984.x 18445157
6. Causton BE (1986) Does the embedding chemistry interact with tissue? In: Müller M, Becker RP, Boyde A, Wolosewick JJ (eds) The science of biological specimen preparation. SEM Inc, AMF O'Hare, IL, pp 209-214
7. P Diakou S Fedou JP Carde C Cassagne P Moreau L Maneta-Peyret 2002 Immunolocalization of long-chain acyl-CoAs in plant cells Biochim Biophys Acta 1583 85 90 1:CAS:528:DC%2BD38XksF2hsb8%3D 12069852
8. M Eltsov KM MacLellan K Maeshimad AS Frangakis J Dubochet 2008 Analysis of cryo-electron microscopy images does not support the existence of 30-nm chromatin fibers in mitotic chromosomes in situ PNAS 105 19732 19737 1:CAS:528:DC%2BD1cXhsFCltrrN 10.1073/pnas.0810057105 19064912
9. SS Ghrebi GR Owen DM Brunette 2007 Triton X-100 pretreatment of LR-White thin sections improves immunofluorescence specificity and intensity Microsc Res Technol 70 555 562 1:CAS:528:DC%2BD2sXosF2gtbc%3D 10.1002/jemt.20422
10. TH Giddings 2003 Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples J Microsc 212 53 61 1:STN:280:DC%2BD3svlsFymuw%3D%3D 10.1046/j.1365-2818.2003.01228.x 14516362
11. Hayat MA (2000) Principles and techniques of electron microscopy. Biological applications. Cambridge University Press, Cambridge
12. MW Hess 1995 High-pressure freeze fixation reveals novel features during ontogenesis of the vegetative cell in Ledebouria pollen: an ultrastructural and cytochemical study Biochem Cell Biol 73 1 10 1:CAS:528:DyaK2MXmsFeru74%3D 10.1139/o95-001 7662307
13. MW Hess 2003 Of plants and other pets: practical aspects of freeze-substitution and resin embedding J Microsc 212 44 52 1:STN:280: DC%2BD3svlsFymug%3D%3D 10.1046/j.1365-2818.2003.01237.x 14516361
14. S Hippe K Düring F Kreuzaler 1989 In situ localization of a foreign protein in transgenic plants by immunoelectron microscopy following high pressure freezing. Freeze substitution and low temperature embedding Eur J Cell Biol 50 230 234 1:CAS:528:DyaK3cXhvVyrsg%3D%3D
15. S Hippe-Sanwald 1993 The impact of freeze-substitution on biological electron microscopy Microsc Res Technol 24 400 422 1:CAS:528:DyaK3sXkvFSgs74%3D 10.1002/jemt.1070240506
16. X Huang Q Zhang 2007 Improvement and observation of immunoelectron microscopic method for the localization of frog Rana grylio virus (RGV) in infected fish cells Micron 38 599 606 1:CAS:528:DC%2BD2sXksVOlsbY%3D 10.1016/j.micron.2006.10.001 17095234
17. Humbel BM, Müller M (1985) Freeze substitution and low temperature embedding. In: Müller M, Becker RP, Boyde A, Wolosewick JJ (eds) The science of biological specimen preparation. SEM Inc, AMF O'Hare, IL, pp 175-183
18. Humbel BM, Schwarz H (1989) Freeze-substitution for immunochemistry. In: Leunissen JLM (ed) Immuno-gold labeling in cell biology. CRC Press, Boca Raton, FL, pp 115-134
19. L Kanerva R Jolanki T Estlander 1997 10 years of patch testing with the (meth)acrylate series Contact Dermat 37 255 258 1:CAS:528:DyaK1cXjsl2ktA%3D%3D 10.1111/j.1600-0536.1997.tb02460.x
20. E Kellenberger 1991 The potential of cryofixation and freeze substitution: observations and theoretical considerations J Microsc 161 183 203 1:STN:280:DyaK3M3ksFGgtQ%3D%3D 1903815
21. SA Lancelle PK Hepler 1989 Immunogold labelling of actin on sections of freeze-substituted plant cells Protoplasma 150 72 74 10.1007/BF01352922
22. A Lazarov 2007 Sensitization to acrylates is a common adverse reaction to artificial fingernails J Eur Acad Dermatol Venereol 21 169 174 1:STN:280:DC%2BD2s%2FksVOmtQ%3D%3D 10.1111/j.1468-3083.2006.01883.x 17243950
23. JE Lonsdale KL McDonald RL Jones 1999 High pressure freezing and freeze substitution reveal new aspects of fine structure and maintain protein antigenicity in barley aleurone cells Plant J 17 221 229 1:CAS:528: DyaK1MXhsV2gsb8%3D 10.1046/j.1365-313X.1999.00362.x
24. G Martens EC Humphrey LG Harrison B Silva-Moreno J Ausio HE Kasinsky 2009 High-pressure freezing of spermiogenic nuclei supports a dynamic chromatin model for the histone-to-protamine transition J Cell Biochem 108 1399 1409 1:CAS:528:DC%2BD1MXhsV2gsrrN 10.1002/jcb.22373 19830786
25. N Matsko M Müller 2005 Epoxy resin as fixative during freeze-substitution J Struct Biol 152 92 103 1:CAS:528:DC%2BD2MXht1ars7%2FM 10.1016/j.jsb.2005.07.005 16214372
26. P Maupin TD Pollard 1983 Improved preservation and staining of HeLa cell actin filaments, clathrin-coated membranes, and other cytoplasmic structures by tannic acid-glutaraldehyde-saponin fixation J Cell Biol 96 51 62 1:CAS:528:DyaL3sXosFKisQ%3D%3D 10.1083/jcb.96.1.51 6186673
27. McDonald K (2007) Cryopreparation methods for electron microscopy of selected model systems. In: McIntosh JR (ed) Methods in cell biology. Elsevier, Academic Press, Amsterdam, pp 23-56
28. KL McDonald MK Morphew 1993 Improved preservation of ultrastructure in difficult-to-fix organisms by high pressure freezing and freeze-substitution. I. Drosophila melanogaster and Strongylocentrotus purpuratus embryos Microsc Res Technol 24 465 473 1:CAS:528:DyaK3sXks1KqsLs%3D 10.1002/jemt.1070240603
29. BF McEwen Ch-E Hsieh AL Mattheyses CL Rieder 1998 A new look at kinetochore structure in vertebrate somatic cells using high-pressure freezing and freeze-substitution Chromosoma 107 366 375 1:CAS:528:DyaK1MXktlWksw%3D%3D 10.1007/s004120050320 9914368
30. P Monaghan N Perusinghe M Müller 1998 High-pressure freezing for immunocytochemistry J Microsc 192 248 258 1:STN:280:DyaK1M7itVaqsA%3D%3D 10.1046/j.1365-2818.1998.00387.x 9923417
31. P Monaghan H Cook P Hawes J Simpson F Tomley 2003 High pressure freezing in the study of animal pathogens J Microsc 212 62 70 1:STN:280: DC%2BD3svlsFylsg%3D%3D 10.1046/j.1365-2818.2003.01245.x 14516363
32. MK Morphew JR McIntosh 2003 The use of filter membranes for high-pressure freezing of cell monolayers J Microsc 212 21 25 1:STN:280: DC%2BD3svlsFymtA%3D%3D 10.1046/j.1365-2818.2003.01231.x 14516358
33. Ch Mühlfeld J Richter 2006 High-pressure freezing and freeze-substitution of rat myocardium for immunogold labelling of connexin 43 Anat Rec A 288 1059 1067
34. Müller M, Moor H (1984) Cryofixation of thick specimens by high pressure freezing. In: Revel JP, Barnard T, Haggis GH (eds) The science of biological specimen preparation. SEM Inc, AMF O'Hare, IL, pp 131-138
35. Müller M, Marti T, Kriz S (1980) Improved structural preservation by freeze-substitution. In: Brederoo P, de Priester W (eds) Proceedings of the 7th European congress on electron microscopy, Leiden, pp 720-271
36. T Müller-Reichert J Mäntler M Srayko E O'Toole 2008 Electron microscopy of the early Caenorhabditis elegans embryo J Microsc 230 297 307 10.1111/j.1365-2818.2008.01985.x 18445160
37. JLAN Murk G Posthuma AJ Koster HJ Geuze AJ Verkleij MJ Kleijmeer BM Humbel 2003 Influence of aldehyde fixation on the morphology of endosomes and lysosomes: quantitative analysis and electron tomography J Microsc 212 81 90 1:STN:280:DC%2BD3svpslGntw%3D%3D 10.1046/j.1365-2818.2003.01238.x 14516365
38. Newman GR, Hobot JA (1989) In: Hayat MA (ed) Colloidal gold: principles, methods, and applications. Academic Press, San Diego, pp 33-45
39. MS Otegui R Capp LA Staehelin 2002 Developing seeds of Arabidopsis store different minerals in two types of vacuoles and in the endoplasmic reticulum Plant Cell 14 1311 1327 1:CAS:528:DC%2BD38Xlt1als7s%3D 10.1105/tpc.010486 12084829
40. S Pfeiffer K Krupinska 2005 New insights in thylakoid membrane organization Plant Cell Physiol 46 1443 1451 1:CAS:528:DC%2BD2MXhtVOlurzF 10.1093/pcp/pci156 16208039
41. VV Philimonenko J Janacek P Hozak 2002 LR White is preferable to Unicryl for immunogold detection of fixation-sensitive nuclear antigens Eur J Histochem 46 359 364 1:CAS:528:DC%2BD3sXhsVGnu7k%3D 12597621
42. T Puhakainen MW Hess P Makela J Svensson P Heino ET Palva 2004 Overexpression of multiple dehydrin genes enhances tolerance to freezing stress in Arabidopsis Plant Mol Biol 54 743 753 1:CAS:528:DC%2BD2cXptlCrtLY%3D 10.1023/B:PLAN.0000040903.66496.a4 15356392
43. S Reipert I Fischer G Wiche 2004 High-pressure freezing of epithelial cells on sapphire coverslips J Microsc 213 81 85 1:STN:280: DC%2BD3srpslCjtA%3D%3D 10.1111/j.1365-2818.2004.01260.x 14678516
44. AM Roch MT Nicolas G Quash 1997 Ultrastructural immunolocalization of polyamines in HeLa cells subjected to fast-freezing fixation and freeze-substitution Histochem Cell Biol 107 303 312 1:CAS:528:DyaK2sXislaqurg%3D 10.1007/s004180050115 9151112
45. E Roche J de la Cuadra V Alegre 2008 Sensitization to acrylates caused by artificial acrylic nails: review of 15 cases Actas Dermosifiliogr 99 788 794 1:STN:280:DC%2BD1M%2FjvVanug%3D%3D 10.1016/S0001-7310(08)74959-7 19091218 (article in Spanish)
46. Roth J (1989) Postembedding labeling on Lowicryl K4M tissue sections: detection and modification of cellular components. In: Tartakoff AM (ed) Methods in cell biology. Academic Press, San Diego, pp 513-551
47. J Roth DJ Taatjes 1998 Tubules of the trans Golgi apparatus visualized by immunoelectron microscopy Histochem Cell Biol 109 545 553 1:CAS:528: DyaK1cXjt1Oksr0%3D 10.1007/s004180050254 9681634
48. J Roth M Bendayan E Carlemalm W Villiger M Garavito 1981 Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue J Histochem Cytochem 29 663 671 1:STN:280: DyaL3M3ktVOjsg%3D%3D 6166664
49. A Sawaguchi KL McDonald S Karvar JG Forte 2002 A new approach for high-pressure freezing of primary culture cells: the fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cells J Microsc 208 158 166 1:STN:280:DC%2BD38npvFKjtA%3D%3D 10.1046/j.1365-2818.2002. 01085.x 12460447
50. A Sawaguchi X Yao JG Forte K McDonald 2003 Direct attachment of cell suspensions to high-pressure freezing specimen planchettes J Microsc 212 13 20 1:STN:280:DC%2BD3svlsFymtw%3D%3D 10.1046/j.1365-2818.2003.01225.x 14516357
51. D Staiger S Kappeler M Muller K Apel 1994 The proteins encoded by two tapetum-specific transcripts, Sa tap35 and Sa tap44, from Sinapis alba L. are localized in the exine cell wall layer of developing microspores Planta 192 221 231 1:CAS:528:DyaK2cXhtVWqur4%3D 10.1007/BF01089038 7764317
52. V Strádalová K Gaplovská-Kyselá P Hozák 2008 Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells Histochem Cell Biol 130 1047 1052 10.1007/s00418-008- 0504-x 18797913
53. D Studer M Michel M Wohlwend EB Hunziker MD Buschmann 1995 Vitrification of articular cartilage by high-pressure freezing J Microsc 179 321 332 1:STN:280:DyaK28%2FntVKqsw%3D%3D 7473694
54. D Studer W Graber A Al-Amoudi P Eggli 2001 A new approach for cryofixation by high-pressure freezing J Microsc 203 285 294 1:CAS:528:DC%2BD3MXntFGgt7w%3D 10.1046/j.1365-2818.2001.00919.x 11555146
55. D Studer BM Humbel M Chiquet 2008 Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution Histochem Cell Biol 130 877 889 1:CAS:528:DC%2BD1cXht1eit7%2FK 10.1007/s00418-008-0500-1 18795316
56. PJ Szczesny P Walther M Muller 1996 Light damage in rod outer segments: the effects of fixation on ultrastructural alterations Curr Eye Res 15 807 814 1:STN:280:DyaK2s%2FnvFGisA%3D%3D 10.3109/02713689609017621 8921223
57. H Takahashi H Kuroiwa S Miyagishima K Toda R Itoh T Kuroiwa 1997 Improved procedure for immunogold electron microscopy: rapid-freeze substitution with absolute acetone Cytologia (Tokyo) 62 303 308
58. T Takizawa T Takizawa S Arai K Kizawa H Uchiwa I Sasaki T Inoue 1999 Ultrastructural localization of S100A3, a cysteine-rich, calcium binding protein, in human scalp hair shafts revealed by rapid-freezing immunocytochemistry J Histochem Cytochem 47 525 532 1:CAS:528:DyaK1MXit1Ogtb0%3D 10082754
59. A Teik-Jin Goon M Bruze E Zimerson CL Goh M Isaksson 2007 Contact allergy to acrylates/methacrylates in the acrylate and nail acrylics series in southern Sweden: simultaneous positive patch test reaction patterns and possible screening allergens Contact Dermat 57 21 27 10.1111/j.1600-0536.2007.01151.x
60. M Tobler AU Freiburghaus 1990 Occupational risks of (meth)acrylate compounds in embedding media for electron microscopy J Microsc 160 291 298 1:CAS:528:DyaK3MXktlCntbo%3D 2084265
61. M Tobler B Wüthrich AU Freiburghaus 1990 Contact dermatitis from acrylate and methacrylate compounds in Lowicryl embedding media for electron microscopy Contact Dermat 23 96 102 1:CAS:528:DyaK3MXktFCmt7g%3D 10.1111/j.1600-0536.1990.tb03230.x
62. P Verkade 2008 Moving EM: the Rapid Transfer System as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing J Microsc 230 317 328 1:STN:280:DC%2BD1c3otleisQ%3D%3D 10.1111/j.1365-2818.2008.01989.x 18445162
63. M-L Von Schack S Fakan W Villiger M Muller 1993 Cryofixation and cryosubstitution: a useful alternative in the analyses of cellular fine structure Eur J Histochem 37 5 18
64. P Walther A Ziegler 2002 Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water J Microsc 208 3 10 1:CAS:528: DC%2BD38XotlOjtLo%3D 10.1046/j.1365-2818.2002.01064.x 12366592
65. C Weibull W Villiger E Carlemalm 1984 Extraction of lipids during freeze-substitution of Acholeplasma laidlawii-cells for electron microscopy J Microsc 134 213 216 1:STN:280:DyaL2c3kt1yitA%3D%3D 6429337
66. P Wild EM Schraner H Adler BM Humbel 2001 Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze-substitution Microsc Res Technol 53 313 321 1:CAS:528:DC%2BD3MXksVyhs7c%3D 10.1002/jemt.1098