Genotoxicity testing of PLGA-PEO nanoparticles in TK6 cells by the comet assay and the cytokinesis-block micronucleus assay

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 748, Issue 1-2, 2012, Pages 42-47

  Kazimirova, Alena ^a   Magdolenova, Zuzana ^b   Barancokova, Magdalena ^a   Staruchova, Marta ^a   Volkovova, Katarina ^a   Dusinska, Maria ^b  

^a SLOVAK MEDICAL UNIVERSITY   (Slovakia)

^b NORWEGIAN INSTITUTE FOR AIR RESEARCH   (Norway)

Author keywords

Genotoxicity; Micronuclei; Nanoparticles; PLGA PEO

Indexed keywords

COPOLYMER; CYTOCHALASIN; NANOPARTICLE; POLY LACTIC CO GLYCOLIC ACID POLYETHYLENE OXIDE; UNCLASSIFIED DRUG;

ARTICLE; COMET ASSAY; CYTOKINESIS BLOCK MICRONUCLEUS TEST; DNA STRAND BREAKAGE; GENOTOXICITY; IN VITRO STUDY; MICRONUCLEATED BINUCLEATED CELL; MICRONUCLEUS; MICRONUCLEUS TEST; PRIORITY JOURNAL;

CELL LINE; COMET ASSAY; DNA DAMAGE; HUMANS; LACTIC ACID; MICRONUCLEUS TESTS; MUTAGENS; NANOPARTICLES; POLYETHYLENE GLYCOLS; POLYGLYCOLIC ACID;

EID: 84865028605     PISSN: 13835718     EISSN: 18793592     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2012.06.012     Document Type: Article

References (28)

1. Soppimath K.S., Aminabhavi T.M., Kulkami A.R., Rudzinski W.E. Biodegradable polymeric nanoparticles as drug delivery devices. J. Control. Release 2001, 70:1-20.
2. ® 65/35 d,l-lactide/glycolide copolymer microspheres. J. Control. Release 1993, 24:81-93.
3. http://www.advancell.net/.
4. Dusinska M., Collins A.R. The Comet assay in human biomonitoring: gene-environment interactions. Mutagenesis 2008, 23:191-205.
5. Collins A.R., Duthie S.J., Dobson V.L. Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis 1993, 14:1733-1735.
6. Dusinska M., Collins A.R. Detection of oxidised purines and UV-induced photoproducts in DNA, by inclusion of lesion-specific enzymes in the comet assay (single cell gel electrophoresis). ATLA 1996, 24:405-411.
7. Collins A.R., Dusinska M., Gedik C.M., Stetina R. Oxidative damage to DNA: do we have a reliable biomarker?. Environ. Health Perspect. 1996, 104:465-469.
8. Smith C.C., Donovan M.R., Martin E.A. hOGG1 recognizes oxidative damage using the comet assay with greater specificity than FPG or ENDOIII. Mutagenesis 2006, 21:185-190.
9. Collins A.R., Dusinska M., Horska A. Detection of alkylation damage in human lymphocyte DNA with the comet assay. Acta Biochim. Polon. 2001, 48:611-614.
10. Fenech M., Bonassi S. The effect of age, gender, diet and lifestyle on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes. Mutagenesis 2011, 26:43-49.
11. OECD, Guideline for the testing of chemicals, in vitro mammalian cell micronucleus test, 487, (2010) Adapted: (22.07.10).
12. Mateuca R., Lombaert N., Aka P.V., Decordier I., Kirsch-Volders M. Chromosomal changes: induction, detection methods and applicability in human biomonitoring. Biochimie 2006, 88:1515-1531.
13. Fenech M. Cytokinesis-block micronucleus cytome assay. Nat. Protoc. 2007, 2:1084-1104.
14. Fenech M., Chang W.P., Kirsch-Volders M., Holland N., Bonassi S., Zeiger E. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res. 2003, 534:65-75.
15. Elhajouji M., Cunha M., KirschVolders M. Spindle poisons can induce polyploidy by mitotic slippage and micronucleate mononucleates in the cytokinesis-block assay. Mutagenesis 1998, 13:193-198.
16. Fenech M. The advantages and disadvantages of the cytokinesis-block micronucleus method. Mutat. Res. 1997, 392:11-18.
17. Pfuhler S., Fellows M., van Benthem J., Corvi R., Curren R., Dearfield K., Fowler P., Frötschl R., Elhajouji A., Le Hégarat L., Kasamatsu T., Kojima H., Ouédraogo G., Scott A., Speit G. In vitro genotoxicity test approaches with better predictivity: summary of an IWGT workshop. Mutat. Res. 2011, 723:101-107.
18. Witte I., Plappert U., de Wall H., Hartmann A. Genetic toxicity assessment: employing the best science for human safety evaluation part III: the comet assay as an alternative to in vitro clastogenicity tests for early drug candidate selection. Toxicol. Sci. 2007, 97:21-26.
19. Magdolenova Z., Lorenzo Y., Collins A., Dusinska M. Can standard genotoxicity tests be applied to nanoparticles?. J Toxicol Environ Health A 2012, 75:800-806.
20. Mansour H.M., Rhee Y.S., Wu X. Nanomedicine in pulmonary delivery. Int. J. Nanomed. 2009, 4:299-319.
21. Acharya S., Sahoo S.K. PLGA nanoparticles containing various anticancer agents and tumour delivery by EPR effect. Adv. Drug. Deliv. Rev. 2011, 63:170-183.
22. Dusinska M., Fjellsbø L., Magdolenova Z., Rinna A., Runden Pran E., Bartonova A., Heimstad E., Harju M., Tran L., Ross B., Juillerat L., Halamoda Kenzaui B., Marano F., Boland S., Guadaginini R., Saunders M., Cartwright L., Carreira S., Whelan M., Kelin Ch., Worth A., Palosaari T., Burello E., Housiadas C., Pilou M., Volkovova K., Tulinska J., Kazimirova A., Barancokova M., Sebekova K., Hurbankova M., Kovacikova Z., Knudsen L., Poulsen M., Mose T., Vilà M., Gombau L., Fernandez B., Castell J., Marcomini A., Pojana G., Bilanicova D., Vallotto D. Testing strategies for the safety of nanoparticles used in medical application. Nanomedicine 2009, 4:605-607.
23. He L., Yang L., Zhang Z., Gong T., Deng L., Gu Z., Sun X. In vitro evaluation of the genotoxicity of a family of novel MeO-PEG-poly(d,l-lactic-co-glycolic acid)-PEG-OMe triblock copolymer and PLGA nanoparticles. Nanotechnology 2009, 20:1-8.
24. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.C., Sasaki Y.F. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 2000, 35:206-221.
25. Angelis J.K., Dusinska M., Collins A.R. Single cell gel electrophoresis: detection of DNA damage at different levels of sensitivity. Electrophoresis 1999, 20:2133-2138.
26. Speit G., Schütz P., Bonzheim I., Trenz K., Hoffmann H. Sensitivity of the FPG protein towards alkylation damage in the comet assay. Toxicol. Lett. 2004, 15:151-158.
27. Gonzalez L., Sanderson B.J., Kirsch-Volders M. Adaptations of the in vitro MN assay for the genotoxicity assessment of nanomaterials. Mutagenesis 2011, 26:185-191.
28. Rosefort Ch., Fauth E., Zankl H. Micronuclei induced by aneugens and clastogens in mononucleate and binucleate cells using the cytokinesis block assay. Mutagenesis 2004, 19:277-284.