Detection of oxidised purines and UV-induced photoproducts in DNA of single cells, by inclusion of Lesion-specific enzymes in the comet assay

Alternatives to Laboratory Animals

Volumn 24, Issue 3, 1996, Pages 405-411

  Dušinská, Mária ^a,b   Collins, Andrew ^c  

^a CANCER RESEARCH INSTITUTE   (Slovakia)

^b INST OF PREV AND CLINICAL MEDICINE   (Slovakia)

^c ROWETT RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Comet assay; DNA damage; Formamidopyrimidine DNA glycosylase; uvrABC excinuclease

Indexed keywords

EID: 2842526228     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Cook, P.R. & Brazell, I.A. (1978). Conformational constraints in nuclear DNA. Journal of Cell Science 22, 287-302.
2. Gedik, C.M., Ewen, S.W.B. & Collins, A.R. (1992). Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. International Journal of Radiation Biology 62, 313-320.
3. Collins, A.R., Duthie, S.J. & Dobson,V.L. (1993). Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA, Carcinogenesis 14, 1733-1735.
4. Boiteux, S., O'Connor, T.R., Lederer, F., Gouyette, A. & Laval, J. (1990). Homogeneous Escherichia coli FPG protein. Journal of Biological Chemistry 265, 3916-3922.
5. Boiteux, S., Gajewski, E., Laval, J. & Dizdaroglu, M. (1992). Substrate specificity of the Escherichia coli FPG protein (formamidopyrimidine-DNA glycosylase): excision of purine lesions in DNA produced by ionizing radiation or photosensitization. Biochemistry 31, 106-110.
6. Sancar, A. & Sancar, G.B. (1988). DNA repair enzymes. Annual Review of Biochemistry 57, 29-67.
7. Singh, N.P., McCoy, M.T., Tice, R.R. & Schneider, E.L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research 175, 184-191.
8. Collins, A.R., Ma, A-G. & Duthie, S.J. (1995). The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutation Research: DNA Repair 336, 69-77
9. Milne, L., Nicotera, P., Orrenius, S. & Burkitt, M.J. (1993). Effects of glutathione and chelating agents on copper mediated DNA oxidation: prooxidant and antioxidant properties of glutathione. Archives of Biochemistry and Biophysics 304, 102-109.
10. Duthie, S.J., Ma, A, Ross, M.A. & Collins, A.R. (1996). Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes. Cancer Research, 56, 1291-1295.
11. Lindahl, T. (1993). Instability and decay of the primary structure of DNA. Nature, London 362, 709-715.