An advanced PCR method for the specific detection of viable total coliform bacteria in pasteurized milk

Applied Microbiology and Biotechnology

Volumn 95, Issue 2, 2012, Pages 485-497

  Soejima, Takashi ^a   Minami, Jun Ichi ^a   Yaeshima, Tomoko ^a   Iwatsuki, Keiji ^a  

^a MORINAGA MILK INDUSTRY CO LTD   (Japan)

Author keywords

Direct real time PCR; Pasteurized milk; Viable coliform bacteria .EMA

Indexed keywords

COLONY FORMING UNITS; DETECTION LIMITS; DNA-TEMPLATE; ETHIDIUM MONOAZIDE; FALSE POSITIVE; GRAM-NEGATIVE BACTERIA; LOW DOSE; PASTEURIZED MILK; PCR METHOD; PROTEINASE K; REAL-TIME PCR; RRNA GENES; SIMPLE PROTOCOL; SPECIFIC DETECTION; TESTING TIME; TOTAL COLIFORM BACTERIA; TOTAL COLIFORMS; VIABLE CELLS; VIABLE COLIFORM BACTERIA .EMA;

BROMINE COMPOUNDS; CELL MEMBRANES; COLIFORM BACTERIA; CYTOLOGY; DNA; GENES; PASTEURIZATION; RNA;

POLYMERASE CHAIN REACTION;

ETHIDIUM BROMIDE; ETHIDIUM MONOAZIDE BROMIDE; PROTEINASE K; RNA 16S; RNA 23S; UNCLASSIFIED DRUG;

COLIFORM BACTERIUM; DETECTION METHOD; ENZYME ACTIVITY; INHIBITOR; MEMBRANE; POLYMERASE CHAIN REACTION; REAL TIME;

ARTICLE; BACTERIAL VIABILITY; BACTERIUM DETECTION; COLIFORM BACTERIUM; COLONY FORMING UNIT; DNA TEMPLATE; FOOD MICROBIOTECHNOLOGY; GENE AMPLIFICATION; MILK; NONHUMAN; NUCLEOTIDE SEQUENCE; PASTEURIZATION; POLYMERASE CHAIN REACTION;

ANIMALS; AZIDES; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; DNA, RIBOSOMAL; ENTEROBACTERIACEAE; ENZYME INHIBITORS; ETHIDIUM; MICROBIAL VIABILITY; MILK; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

NEGIBACTERIA;

EID: 84864652039     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4086-0     Document Type: Article

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