Specific detection of viable legionella cells by combined use of photoactivated ethidium monoazide and PCR/real-time PCRδ

Applied and Environmental Microbiology

Volumn 75, Issue 1, 2009, Pages 147-153

  Chang, Bin ^a   Sugiyama, Kanji ^b   Taguri, Toshitsugu ^c   Amemura Maekawa, Junko ^a   Kura, Fumiaki ^a   Watanabe, Haruo ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b SHIZUOKA INSTITUTE OF ENVIRONMENT AND HYGIENE   (Japan)

^c Nagasaki Prefectural Institute for Environmental Research and Public Health   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFIED FRAGMENTS; DEAD CELLS; ENVIRONMENTAL SURVEILLANCES; ETHIDIUM MONOAZIDE; LEGIONELLA; LEGIONELLOSIS; PCR ASSAYS; PHOTO ACTIVATED; RAPID DETECTION METHODS; SPECIFIC DETECTIONS; VIABLE CELLS; WATER SAMPLES; WATER SYSTEMS;

CHLORINE; COSMIC RAYS; CYTOLOGY; DNA; GENES; INFORMATION DISSEMINATION; NUCLEIC ACIDS; ORGANIC ACIDS;

CELLS;

ASSAY; BACTERIUM; CYTOLOGY; DETECTION METHOD; POLYMERASE CHAIN REACTION; REAL TIME;

AZIDES; HUMANS; LEGIONELLA; MALE; MICROBIAL VIABILITY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; WATER MICROBIOLOGY;

LEGIONELLA;

EID: 58149340249     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00604-08     Document Type: Article

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