Development of a real-time PCR for detection of Mycoplasma bovis in bovine milk and lung samples

Journal of Veterinary Diagnostic Investigation

Volumn 17, Issue 6, 2005, Pages 537-545

  Cai, Hugh Y ^a   Bell Rogers, Patricia ^a   Parker, Lois ^a   Prescott, John F ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Bovine; Mastitis; Mycoplasma agalactiae; Mycoplasma bovis; Real time PCR

Indexed keywords

ANIMALIA; BACTERIA (MICROORGANISMS); BOS TAURUS; BOVINAE; MYCOPLASMA; MYCOPLASMA AGALACTIAE; MYCOPLASMA BOVIS;

EID: 27944489553     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063870501700603     Document Type: Article

References (31)

1. Bashiruddin JB, Frey J, Konigsson MH, et al.: 2005, Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial. Vet J 169:268-275.
2. Biddle MK, Fox LK, Hancock DD, et al.: 2004, Effects of storage time and thawing methods on the recovery of Mycoplasma species in milk samples from cows with intramammary infections. J Dairy Sci 87:933-936.
3. Chavez Gonzalez YR, Ros Bascunana C, Bolske G, et al.: 1995, In vitro amplification of the 16S rRNA genes from Mycoplasma bovis and Mycoplasma agalactiae by PCR. Vet Microbiol 47:183-190.
4. Chen DP, Tseng CP, Lin HT, Sun CF: 2005, Application of real-time PCR and melting curve analysis in rapid detection of Ael and Bel blood types. Ann Clin Lab Sci 35:25-30.
5. Deindl P, Peri-Jerkan S, Deichmann K, et al.: 2005, No association of histamine-N-methyltransferase polymorphism with asthma or bronchial hyperresponsiveness in two German pediatric populations. Pediatr Allergy Immunol 16:40-42.
6. Didenko VV: 2001, DNA probes using fluorescence resonance energy transfer (FRET): designs and applications. Biotechniques 31:1106-1116, 1118, 1120-1121.
7. Foddai A, Idini G, Fusco M, et al.: 2005, Rapid differential diagnosis of Mycoplasma agalactiae and Mycoplasma bovis based on a multiplex-PCR and a PCR-RFLP. Mol Cell Probes 19:207-212.
8. Fox LK, Hancock DD, Mickelson A, Britten A: 2003, Bulk tank milk analysis: factors associated with appearance of Mycoplasma sp. in milk. J Vet Med B Infect Dis Vet Public Health 50:235-240.
9. Ghadersohi A: 2003, Development of a PCR for specific detection of Mycoplasma bovis from bovine milk and mucosal samples: a critique. Vet Microbiol 97:167-168; author reply 169-171.
10. Ghadersohi A, Coelen RJ, Hirst RG: 1997, Development of a specific DNA probe and PCR for the detection of Mycoplasma bovis. Vet Microbiol 56:87-98.
11. Ghadersohi A, Hirst RG, Forbes-Faulkener J, Coelen RJ: 1999, Preliminary studies on the prevalence of Mycoplasma bovis mastitis in dairy in cattle in Australia. Vet Microbiol 65:185-194.
12. Hayman B: 2003, Development of a PCR for specific detection of Mycoplasma bovis from bovine milk and mucosal samples. Vet Microbiol 97:169-171.
13. Hayman B, Hirst R: 2003, Development of a semi-nested PCR for the improved detection of Mycoplasma bovis from bovine milk and mucosal samples. Vet Microbiol 91:91-100.
14. Hirose K, Kawasaki Y, Kotani K, et al.: 2001, Detection of mycoplasma in mastitic milk by PCR analysis and culture method. J Vet Med Sci 63:691-693.
15. Hotzel H, Heller M, Sachse K: 1999, Enhancement of Mycoplasma bovis detection in milk samples by antigen capture prior to PCR. Mol Cell Probes 13:175-178.
16. Hotzel H, Sachse K, Pfutzner H: 1996, Rapid detection of Mycoplasma bovis in milk samples and nasal swabs using the polymerase chain reaction. J Appl Bacteriol 80:505-510.
17. Konigsson MH, Bolske G, Johansson KE: 2002, Intraspecific variation in the 16S rRNA gene sequences of Mycoplasma agolactiae and Mycoplasma bovis strains. Vet Microbiol 85:209-220.
18. Lyon E: 2001, Mutation detection using fluorescent hybridization probes and melting curve analysis. Expert Rev Mol Diagn 1:92-101.
19. Marenda MS, Sagne E, Poumarat F, Citti C: 2005, Suppression subtractive hybridization as a basis to assess Mycoplasma agalactiae and Mycoplasma bovis genomic diversity and species-specific sequences. Microbiology 151:475-489.
20. Nauck M, Hoffmann MM, Wieland H, Marz W: 2000, Evaluation of the apo E genotyping kit on the LightCycler. Clin Chem 46:722-724.
21. Nicholas RA, Ayling RD: 2003, Mycoplasma bovis: disease, diagnosis, and control. Res Vet Sci 74:105-112.
22. Natioal Mastitis Council (NMC): 1999, National Mastitis Council: laboratory handbook on bovine mastitis. NMC, Verona, WI.
23. Pinnow CC, Butler JA, Sachse K, et al.: 2001, Detection of Mycoplasma bovis in preservative-treated field milk samples. J Dairy Sci 84:1640-1645.
24. Rosengarten L, Citti C: 1999, The role of ruminant mycoplasmas in systemic infection. In: Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics, eds. Stipkovits L, Rosengarten L, Frey J, vol 3., pp. 14-17. European Commission Directorate-General XII Science Research and Development, Luxembourg.
25. Ruhnke HL, Rosendal S: 1994, Useful protocols for diagnosis of animal mycoplasmas. In: Mycoplasmosis in animals: laboratory diagnosis, eds. Whitford HW, Rosenbusch RF, Lauerman LH, 1st ed., pp. 141-166. Iowa State University Press, Ames, IA.
26. Schroter M, Zollner B, Schafer P, et al.: 2002, Genotyping of hepatitis C virus types 1,2,3, and 4 by a one-step LightCycler method using three different pairs of hybridization probes. J Clin Microbiol 40:2046-2050.
27. Subramaniam S, Bergonier D, Poumarat F, et al.: 1998, Species identification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvrC genes by PCR. Mol Cell Probes 12:161-169.
28. Tamer L, Calikoglu M, Ates NA, et al.: 2004, Glutathione-S-transferase gene polymorphisms (GSTT1, GSTM1, GSTP1) as increased risk factors for asthma. Respirology 9:493-498.
29. Thomas A, Dizier I, Linden A, et al.: 2004, Conservation of the uvrC gene sequence in Mycoplasma bovis and its use in routine PCR diagnosis. Vet J 168:100-102.
30. Thomas A, Linden A, Mainil J, et al.: 2005, Mycoplasma bovis shares insertion sequences with Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides SC: evolutionary and developmental aspects. FEMS Microbiol Lett 245:249-255.
31. Wilson DJ, Gonzalez RN, Das HH: 1997, Bovine mastitis pathogens in New York and Pennsylvania: prevalence and effects on somatic cell count and milk production. J Dairy Sci 80:2592-2598.