Photoactivated ethidium monoazide directly cleaves bacterial DNA and is applied to PCR for discrimination of live and dead bacteria

Microbiology and Immunology

Volumn 51, Issue 8, 2007, Pages 763-775

  Soejima, Takashi ^a,b   Iida, Ken Ichiro ^a   Qin, Tian ^a   Taniai, Hiroaki ^a   Seki, Masanori ^a   Takade, Akemi ^a   Yoshida, Shin Ichi ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b MORINAGA MILK INDUSTRY CO LTD   (Japan)

Author keywords

Bactericide; DNA cleavage; Ethidium monoazide; PCR

Indexed keywords

BACTERIAL DNA; DNA TOPOISOMERASE (ATP HYDROLYSING); DNA TOPOISOMERASE IV; ETHIDIUM; ETHIDIUM AZIDE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERICIDAL ACTIVITY; CONTROLLED STUDY; DNA CLEAVAGE; DNA STRAND BREAKAGE; DOSE RESPONSE; ELECTRON MICROSCOPY; ESCHERICHIA COLI; NONHUMAN; PHOTOACTIVATION; PHOTOLYSIS; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); EUKARYOTA; KLEBSIELLA OXYTOCA;

EID: 34548237732     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2007.tb03966.x     Document Type: Article

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