Specific fluorine labeling of the HyHEL10 antibody affects antigen binding and dynamics

Biochemistry

Volumn 51, Issue 30, 2012, Pages 6017-6027

  Acchione, Mauro ^a,d   Lee, Yi Chien ^b,e   Desantis, Morgan E ^a,f   Lipschultz, Claudia A ^a   Wlodawer, Alexander ^b   Li, Mi ^b,c   Shanmuganathan, Aranganathan ^a,g   Walter, Richard L ^a,h   Smith Gill, Sandra ^a   Barchi, Joseph J ^b  

^a NATIONAL CANCER INSTITUTE   (United States)

^b FREDERICK NATIONAL LABORATORY FOR CANCER RESEARCH   (United States)

^c SAIC FREDERICK INC   (United States)

^d UNIVERSITY OF WASHINGTON   (United States)

^e GEORGETOWN UNIVERSITY   (United States)

^f UNIVERSITY OF PENNSYLVANIA   (United States)

^g RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^h UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY MATURATION; ANTIBODY-ANTIGEN; ANTIGEN BINDING; BINDING AFFINITIES; BINDING INTERFACE; BIOPHYSICAL TECHNIQUES; CHEMICAL ENVIRONMENT; CHEMICAL EXCHANGE; EGG WHITE; FLUORINE SUBSTITUTION; GAIN INSIGHT; MOLECULAR MECHANISM; NMR ANALYSIS; PROTEIN STRUCTURES; RELAXATION ANALYSIS; SITE-SPECIFIC; STRUCTURAL CHANGE; TRYPTOPHAN RESIDUES;

AMINO ACIDS; ANTIGENS; BINDING ENERGY; CHEMICAL ANALYSIS; FLUORINE; TUNGSTEN; TUNGSTEN COMPOUNDS; X RAY CRYSTALLOGRAPHY;

ANTIBODIES;

FLUORINE; LYSOZYME; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TRYPTOPHAN;

ANTIGEN ANTIBODY COMPLEX; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CHEMICAL ENVIRONMENT; CONTROLLED STUDY; CRYSTAL STRUCTURE; FLUORINE NUCLEAR MAGNETIC RESONANCE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; SIGNAL TRANSDUCTION; X RAY CRYSTALLOGRAPHY;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS; BINDING SITES, ANTIBODY; CRYSTALLOGRAPHY, X-RAY; FLUORINE; IMMUNOGLOBULIN G; ISOTOPE LABELING; MICE; MOLECULAR DYNAMICS SIMULATION; MURAMIDASE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY;

EID: 84864464325     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300455t     Document Type: Article

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