Low level sequence variant analysis of recombinant proteins: An optimized approach

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Zeck, Anne ^a,c   Regula, Jörg Thomas ^a   Larraillet, Vincent ^a   Mautz, Björn ^a   Popp, Oliver ^a   Göpfert, Ulrich ^a   Wiegeshoff, Frank ^a,b   Vollertsen, Ulrike E E ^a   Gorr, Ingo H ^a   Koll, Hans ^a   Papadimitriou, Apollon ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

^b ABBOTT GMBH AND CO KG   (Germany)

^c UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; ASPARAGINE; CYTOSINE; GLYCOPEPTIDE; IMMUNOGLOBULIN G1 ANTIBODY; ISOLEUCINE; LEUCINE; MONOCLONAL ANTIBODY; PHENYLALANINE; RECOMBINANT PROTEIN; THREONINE; TYROSINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL DENSITY; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; FED BATCH FERMENTATION; FEMALE; FERMENTATION MEDIUM; GLYCOSYLATION; ISOMER; MUTATIONAL ANALYSIS; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN DETERMINATION; PROTEIN PROCESSING; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY ANALYSIS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; STOP CODON; STRUCTURE ACTIVITY RELATION; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; CHO CELLS; CHROMATOGRAPHY, GEL; CRICETINAE; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; IMMUNOGLOBULIN G; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PHENYLALANINE; POINT MUTATION; PROTEOLYSIS; RECOMBINANT PROTEINS; REFERENCE STANDARDS; SEQUENCE ANALYSIS, DNA; SEQUENCE ANALYSIS, PROTEIN; SOFTWARE; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84863707105     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0040328     Document Type: Article

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