Control of misincorporation of serine for asparagine during antibody production using CHO cells

Biotechnology and Bioengineering

Volumn 107, Issue 1, 2010, Pages 116-123

  Khetan, Anurag ^a   Huang, Yao Ming ^a   Dolnikova, Jana ^a   Pederson, Nels E ^a   Wen, Dingyi ^a   Yusuf Makagiansar, Helena ^a   Chen, Paul ^a   Ryll, Thomas ^a  

^a BIOGEN   (United States)

Author keywords

Cell culture; Misincorporation; Substitution

Indexed keywords

AMINO ACID SEQUENCE; ANTIBODY PRODUCTION; CHINESE HAMSTER OVARY CELLS; CHO CELL; FED-BATCH CULTURES; FED-BATCH PROCESS; LOW LEVEL; MANUFACTURING PROCESS; MISINCORPORATION; PEPTIDE MAPPING; RECOMBINANT MONOCLONAL ANTIBODY; ROOT CAUSE; SHAKE-FLASK EXPERIMENTS; STARVATION CONDITIONS; SUBSTITUTION; TIME-COURSE ANALYSIS;

AMINO ACIDS; BATCH DATA PROCESSING; CELL CULTURE; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; ORGANIC ACIDS; PHOSPHATASES;

BATCH CELL CULTURE;

ASPARAGINE; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY; SERINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; BIOREACTOR; CHO CELL; CONTROLLED STUDY; DRUG SYNTHESIS; FED BATCH CULTURE; MASS SPECTROMETRY; NONHUMAN; PEPTIDE MAPPING; SCALE UP;

AMINO ACID SUBSTITUTION; ANIMALS; ANTIBODIES, MONOCLONAL; ASPARAGINE; CHO CELLS; CRICETINAE; CRICETULUS; PROTEIN ENGINEERING; SERINE;

CRICETULUS GRISEUS;

EID: 77956257160     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22771     Document Type: Article

References (22)

1. Andrulis IL, Hatfield GW, Arfin SM. 1979. Asparaginyl transfer RNA aminoacylation levels and asparagine synthetase expression in cultured Chinese-Hamster ovary cells. J Biol Chem 254(21):629-633.
2. Bogosian G, Violand BN, Dorwardking EJ, Workman WE, Jung PE, Kane JF. 1989. Biosynthesis and incorporation into protein of norleucine by Escherichia coli. J Biol Chem 264(1):531-539. (Pubitemid 19027637)
3. Calderone TL, Stevens RD, Oas TG. 1996. High-level misincorporation of lysine for arginine at AGA codons in a fusion protein expressed in Escherichia coli. J Mol Biol 262(4):407-412.
4. Dorai H, Sauerwald T, Campbell A, Kyung YS, Goldstein J, Magill A, Lewis MJ, Tang QM, Jan D, Ganguly S, Moore G. 2007. Investigation of product microheterogeneity: A case study in rapid detection of mutation in mammalian production cell lines. Bioprocess Int 5(8):66-72.
5. Harris RJ, Murnane AA, Utter SL, Wagner KL, Cox ET, Polastri GD, Helder JC, Sliwkowski MB. 1993. Assessing genetic-heterogeneity in production cell-lines - Detection by peptide-mapping of a low-level Tyr to Gln sequence variant in a recombinant antibody. Nat Biotechnol 11(11):1293-1297.
6. Henderson JW, Ricker RD, Bidlingmeyer BA, Woodward C. 2000. Rapid, accurate, sensitive and reproducible analysis of amino acids. Palo Alto, CA: Agilent Technologies. Agilent Publication Number 5980-1193 EN.
7. Kane JF. 1995. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr Opin Biotechnol 6(5):494-500.
8. Laughrea M, Latulippe J, Filion AM, Boulet L. 1987. Mistranslation in 12 Escherichia coli ribosomal-proteins - Cysteine misincorporation at neutral amino-acid-residues other than tryptophan. Eur J Biochem 169(1):59-64.
9. Lu HS, Tsai LB, Kenney WC, Lai PH. 1988. Identification of unusual replacement of methionine by norleucine in recombinant interleukin-2 produced by Escherichia coli. Biochem Biophys Res Commun 156(2):807-813.
10. Lu HS, Fausset PR, Sotos LS, Clogston CL, Rohde MF, Stoney KS, Herman AC. 1993. Isolation and characterization of three recombinant human granulocyte colony stimulating factor his → gln isoforms produced in Escherichia coli. Protein Expression Purif 4(5):465-472.
11. McNulty DE, Claffee BA, Huddleston MJ, Kane JF. 2003. Mistranslational errors associated with the rare arginine codon CGG in Escherichia coli. Protein Expression Purif 27(2):365-374.
12. Parker J, Pollard JW, Friesen JD, Stanners CP. 1978. Stuttering - High-level mistranslation in animal and bacterial-cells. Proc Natl Acad Sci USA 75(3):1091-1095. (Pubitemid 8305213)
13. Petersen LA, Hughes DL, Hughes R, DiMichele L, Salmon P, Connors N. 2001. Effects of amino acid and trace element supplementation on pneumocandin production by Glarea lozoyensis: Impact on titer, analogue levels, and the identification of new analogues of pneumocandin B(0). J Ind Microbiol Biotechnol 26(4):216-221.
14. Prentice HL, Ehrenfels BN, Sisk WP. 2007. Improving performance of mammalian cells in fed-batch processes through "bioreactor evolution". Biotechnol Prog 23:458-464.
15. Rosenberger RF, Holliday R. 1993. Recombinant therapeutic proteins and translational errors. Trends Biotechnol 11(12):498-499. (Pubitemid 23360689)
16. Santos MAS, Tuite MF. 1993. New insights into messenger-RNA decoding - Implications for heterologous protein-synthesis. Trends Biotechnol 11(12):500-505.
17. Schneider M, Marison IW, Stockar U. 1996. The importance of ammonia in mammalian cell culture. J Biotechnol 46(3):161-185.
18. Schneider EL, King DS, Marletta MA. 2005. Amino acid substitution and modification resulting from Escherichia coli expression of recombinant Plasmodium falciparum histidine-rich protein II. Biochemistry 44(3):987-995.
19. Seetharam R, Heeren RA, Wong EY, Braford SR, Klein BK, Aykent S, Kotts CE, Mathis KJ, Bishop BF, Jennings MJ. 1988. Mistranslation in IGF-1 during over-expression of the protein in Escherichia coli using a synthetic gene containing low frequency codons. Biochem Biophys Res Commun 155(1):518-523.
20. Tsai LB, Lu HS, Kenney WC, Curless CC, Klein ML, Lai PH, Fenton DM, Altrock BW, Mann MB. 1988. Control of misincorporation of de novo synthesized norleucine into recombinant interleukin-2 in Escherichia coli. Biochem Biophys Res Commun 156(2):733-739.
21. Wen D, Vecchi MM, Gu S, Su L, Dolnikova J, Huang YM, Foley SF, Garber E, Pederson N, Meier W. 2009. Discovery and investigation of misincorporation of serine at asparagine positions in recombinant proteins expressed in CHO cells. J Biol Chem 284(47):32686-32694.
22. Yu XC, Borisov OV, Alvarez M, Michels DA, Wang YJ, Ling V. 2009. Identification of codon-specific serine to asparagine mistranslation in recombinant monoclonal antibodies by high-resolution mass spectrometry. Anal Chem 81(22):9282-9290.