Coordinate lentiviral expression of Cre recombinase and RFP/EGFP mediated by FMDV 2A and analysis of Cre activity

Journal of Cellular Biochemistry

Volumn 113, Issue 9, 2012, Pages 2909-2919

  Liu, Kuancan ^a,b   Wang, Heng ^a   Long, Ying ^a   Ye, Jun ^a   Yuan, Li ^a  

^a XIAMEN UNIVERSITY   (China)

^b UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

cre recombinase; fluorescent reporter; FMDV 2A; lentiviral vector

Indexed keywords

CRE RECOMBINASE; VIRUS VECTOR;

ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; DRUG ACTIVITY; ENZYME ACTIVITY; FLUORESCENCE MICROSCOPY; FOOT AND MOUTH DISEASE VIRUS; HUMAN; HUMAN CELL; LENTIVIRINAE; LOXP SITE; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; VIRUS EXPRESSION; VIRUS INFECTION;

FOOT-AND-MOUTH DISEASE VIRUS; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; INTEGRASES; LENTIVIRUS;

FOOT-AND-MOUTH DISEASE VIRUS;

EID: 84863681669     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.24168     Document Type: Article

References (41)

1. Ahmed BY, Chakravarthy S, Eggers R, Hermens WT, Zhang JY, Niclou SP, Levelt C, Sablitzky F, Anderson PN, Lieberman AR, Verhaagen J,. 2004. Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors. BMC Neurosci 5: 4.
2. Ansari IH, Chen LM, Liang D, Gil LH, Zhong W, Donis RO,. 2004. Involvement of a bovine viral diarrhea virus NS5B locus in virion assembly. J Virol 78: 9612-9623.
3. Bordonaro M,. 2009. Modular Cre/lox system and genetic therapeutics for colorectal cancer. J Biomed Biotechnol 2009: 358230.
4. Brault V, Besson V, Magnol L, Duchon A, Herault Y,. 2007. Cre/LoxP-mediated chromosome engineering of the mouse genome. Handb Exp Pharmacol 178: 29-48.
5. Casanova E, Fehsenfeld S, Lemberger T, Shimshek DR, Sprengel R, Mantamadiotis T,. 2002. ER-based double iCre fusion protein allows partial recombination in forebrain. Genesis 34: 208-214.
6. Chinnasamy D, Milsom MD, Shaffer J, Neuenfeldt J, Shaaban AF, Margison GP, Fairbairn LJ, Chinnasamy N,. 2006. Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI. Virol J 3: 14.
7. Cronican JJ, Thompson DB, Beier KT, McNaughton BR, Cepko CL, Liu DR,. 2010. Potent delivery of functional proteins into Mammalian cells in vitro and in vivo using a supercharged protein. ACS Chem Biol 5: 747-752.
8. de Felipe P, Ryan MD,. 2004. Targeting of proteins derived from self-processing polyproteins containing multiple signal sequences. Traffic 5: 616-626.
9. de Felipe P, Martin V, Cortes ML, Ryan M, Izquierdo M,. 1999. Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy. Gene Ther 6: 198-208.
10. de Felipe P, Luke GA, Hughes LE, Gani D, Halpin C, Ryan MD,. 2006. E unum pluribus: Multiple proteins from a self-processing polyprotein. Trends Biotechnol 24: 68-75.
11. Donnelly ML, Hughes LE, Luke G, Mendoza H, ten Dam E, Gani D, Ryan MD,. 2001. The "cleavage" activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring "2A-like" sequences. J Gen Virol 82: 1027-1041.
12. Douin V, Bornes S, Creancier L, Rochaix P, Favre G, Prats AC, Couderc B,. 2004. Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors. BMC Biotechnol 4: 16.
13. Furler S, Paterna JC, Weibel M, Bueler H,. 2001. Recombinant AAV vectors containing the foot and mouth disease virus 2A sequence confer efficient bicistronic gene expression in cultured cells and rat substantia nigra neurons. Gene Ther 8: 864-873.
14. Groot Bramel-Verheije MH, Rottier PJ, Meulenberg JJ,. 2000. Expression of a foreign epitope by porcine reproductive and respiratory syndrome virus. Virology 278: 380-389.
15. Gu H, Marth JD, Orban PC, Mossmann H, Rajewsky K,. 1994. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265: 103-106.
16. Hasegawa K, Cowan AB, Nakatsuji N, Suemori H,. 2007. Efficient multicistronic expression of a transgene in human embryonic stem cells. Stem Cells 25: 1707-1712.
17. Hennecke M, Kwissa M, Metzger K, Oumard A, Kroger A, Schirmbeck R, Reimann J, Hauser H,. 2001. Composition and arrangement of genes define the strength of IRES-driven translation in bicistronic mRNAs. Nucleic Acids Res 29: 3327-3334.
18. Kaspar BK, Vissel B, Bengoechea T, Crone S, Randolph-Moore L, Muller R, Brandon EP, Schaffer D, Verma IM, Lee KF, Heinemann SF, Gage FH,. 2002. Adeno-associated virus effectively mediates conditional gene modification in the brain. Proc Natl Acad Sci USA 99: 2320-2325.
19. Kuhn R, Schwenk F, Aguet M, Rajewsky K,. 1995. Inducible gene targeting in mice. Science 269: 1427-1429.
20. Lee G, Saito I,. 1998. Role of nucleotide sequences of LoxP spacer region in Cre-mediated recombination. Gene 216: 55-65.
21. Lee JY, Ristow M, Lin X, White MF, Magnuson MA, Hennighausen L,. 2006. RIP-Cre revisited, evidence for impairments of pancreatic beta-cell function. J Biol Chem 281: 2649-2653.
22. Lengler J, Holzmuller H, Salmons B, Gunzburg WH, Renner M,. 2005. FMDV-2A sequence and protein arrangement contribute to functionality of CYP2B1-reporter fusion protein. Anal Biochem 343: 116-124.
23. Loonstra A, Vooijs M, Beverloo HB, Allak BA, van Drunen E, Kanaar R, Berns A, Jonkers J,. 2001. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Proc Natl Acad Sci USA 98: 9209-9214.
24. Ma C, Mitra A,. 2002. Expressing multiple genes in a single open reading frame with the 2A region of foot-and-mouth disease virus as a linker Mol Breed 9: 191-199.
25. Milsom MD, Woolford LB, Margison GP, Humphries RK, Fairbairn LJ,. 2004. Enhanced in vivo selection of bone marrow cells by retroviral-mediated coexpression of mutant O6-methylguanine-DNA-methyltransferase and HOXB4. Mol Ther 10: 862-873.
26. Mizuguchi H, Xu Z, Ishii-Watabe A, Uchida E, Hayakawa T,. 2000. IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector. Mol Ther 1: 376-382.
27. Nagy A, Mar L, Watts G,. 2009. Creation and use of a Cre recombinase transgenic database. Methods Mol Biol 530: 365-378.
28. Naldini L, Verma IM,. 2000. Lentiviral vectors. Adv Virus Res 55: 599-609.
29. Oh H, Bradfute SB, Gallardo TD, Nakamura T, Gaussin V, Mishina Y, Pocius J, Michael LH, Behringer RR, Garry DJ, Entman ML, Schneider MD,. 2003. Cardiac progenitor cells from adult myocardium: Homing, differentiation, and fusion after infarction. Proc Natl Acad Sci USA 100: 12313-12318.
30. Oh-McGinnis R, Jones MJ, Lefebvre L,. 2010. Applications of the site-specific recombinase Cre to the study of genomic imprinting. Brief Funct Genomics 9: 281-293.
31. Pfeifer A, Brandon EP, Kootstra N, Gage FH, Verma IM,. 2001. Delivery of the Cre recombinase by a self-deleting lentiviral vector: Efficient gene targeting in vivo. Proc Natl Acad Sci USA 98: 11450-11455.
32. Ray MK, Fagan SP, Brunicardi FC,. 2000. The Cre-LoxP system: A versatile tool for targeting genes in a cell- and stage-specific manner. Cell Transplant 9: 805-815.
33. Ryan MD, Drew J,. 1994. Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein. EMBO J 13: 928-933.
34. Ryan MD, King AM, Thomas GP,. 1991. Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence. J Gen Virol 72 (Pt 11): 2727-2732.
35. Sauer B,. 1993. Manipulation of transgenes by site-specific recombination: Use of Cre recombinase. Methods Enzymol 225: 890-900.
36. Sauer B, Henderson N,. 1988. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. Proc Natl Acad Sci USA 85: 5166-5170.
37. Silver DP, Livingston DM,. 2001. Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity. Mol Cell 8: 233-243.
38. Speck NA, Iruela-Arispe ML,. 2009. Conditional Cre/LoxP strategies for the study of hematopoietic stem cell formation. Blood Cells Mol Dis 43: 6-11.
39. Szymczak AL, Workman CJ, Wang Y, Vignali KM, Dilioglou S, Vanin EF, Vignali DA,. 2004. Correction of multi-gene deficiency in vivo using a single "self-cleaving" 2A peptide-based retroviral vector. Nat Biotechnol 22: 589-594.
40. Tang W, Ehrlich I, Wolff SB, Michalski AM, Wolfl S, Hasan MT, Luthi A, Sprengel R,. 2009. Faithful expression of multiple proteins via 2A-peptide self-processing: A versatile and reliable method for manipulating brain circuits. J Neurosci 29: 8621-8629.
41. Wang X,. 2009. Cre transgenic mouse lines. Methods Mol Biol 561: 265-273.