IRES-Dependent Second Gene Expression Is Significantly Lower Than Cap-Dependent First Gene Expression in a Bicistronic Vector

Molecular Therapy

Volumn 1, Issue 4, 2000, Pages 376-382

  Mizuguchi, Hiroyuki ^a   Xu, Zhili ^a   Ishii Watabe, Akiko ^a   Uchida, Eriko ^a   Hayakawa, Takao ^a  

^a NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

Author keywords

Bicistronic vector; EMCV; Gene therapy; IRES

Indexed keywords

ALKALINE PHOSPHATASE; CAPPED RNA; CHLORAMPHENICOL ACETYLTRANSFERASE; LUCIFERASE;

5' UNTRANSLATED REGION; ANIMAL; ARTICLE; CHO CELL; COMPARATIVE STUDY; ENCEPHALOMYOCARDITIS VIRUS; GENE; GENE EXPRESSION; GENE TRANSFER; GENE VECTOR; GENETICS; HAMSTER; HELA CELL; HUMAN; L CELL; LIVER; METABOLISM; MOUSE; PLASMID;

5' UNTRANSLATED REGIONS; ALKALINE PHOSPHATASE; ANIMALS; CHLORAMPHENICOL O-ACETYLTRANSFERASE; CHO CELLS; CRICETINAE; ENCEPHALOMYOCARDITIS VIRUS; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENES; GENETIC VECTORS; HELA CELLS; HUMANS; L CELLS (CELL LINE); LIVER; LUCIFERASES; MICE; PLASMIDS; RNA CAPS;

EID: 0034170820     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1006/mthe.2000.0050     Document Type: Article

References (34)

1. Mountford P. S., Smith A. G. Internal ribosome entry site and dicistronic RNAs in mammalian transgenesis. Trends Genet. 11:1995;179-184.
2. Pelletier J., Sonenberg N. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature. 334:1988;320-325.
3. Jang S. K., Krausslich H. G., Nicklin M. J., Duke G. M., Palmenberg A. C., Wimmer E. A segment of the 5′ nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. J. Virol. 62:1988;2636-2643.
4. Jang S. K., Davies M. V., Kaufman R. J., Wimmer E. Initiation of protein synthesis by internal entry of ribosomes into the 5′ nontranslated region of encephalomyocarditis virus RNA in vivo. J. Virol. 63:1989;1651-1660.
5. Borman A. M., Bailly J. L., Girard M., Kean K. M. Picornavirus internal ribosome entry segments: Comparison of translation efficiency and the requirements for optimal internal initiation of translation in vitro. Nucleic Acids Res. 23:1995;3656-3663.
6. Borman A. M., Mercier P. L., Girard M., Kean K. M. Comparison of picornaviral IRES-driven internal initiation of translation in cultured cells of different origins. Nucleic Acids Res. 25:1997;925-932.
7. Huez I., Creancier L., Audigier S., Gensac M. C., Prats A. C., Prats H. Two independent internal ribosome entry sites are involved in translation initiation of vascular endothelial growth factor mRNA. Mol. Cell. Biol. 18:1989;6178-6190.
8. Emerman M., Temin H. M. Genes with promoters in retrovirus vectors can be independently suppressed by an epigenetic mechanism. Cell. 39:1984;459-467.
9. Emerman M., Temin H. M. Quantitative analysis of gene suppression in integrated retrovirus vectors. Mol. Cell. Biol. 6:1986;792-800.
10. Cullen B. R., Lomedico P. T., Ju G. Transcriptional interference in avian retroviruses - Implications for the promoter insertion model of leukaemogenesis. Nature. 307:1984;241-245.
11. Ghattas I. R., Sanes J. R., Majors J. E. The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos. Mol. Cell. Biol. 11:1991;5848-5859.
12. Morgan R. A., Couture L., Elroy-Stein O., Ragheb J., Moss B., Anderson W. F. Retroviral vectors containing putative internal ribosome entry sites: Development of a polycistronic gene transfer system and applications to human gene therapy. Nucleic Acids Res. 20:1992;1293-1299.
13. Adam M. A., Ramesh N., Miller A. D., Osborne W. R. Internal initiation of translation in retroviral vectors carrying picornavirus 5′ nontranslated regions. J. Virol. 65:1991;4985-4990.
14. Okada H., Miyamura K., Itoh T., Hagiwara M., Wakabayashi T., Mizuno M., Colosi P., Kurtzman G., Yoshida J. Gene therapy against an experimental glioma using adeno-associated virus vectors. Gene Ther. 3:1996;957-964.
15. Rees S., Coote J., Stables J., Goodson S., Harris S., Lee M. G. Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotic-resistant cells to express recombinant protein. Biotechniques. 106:1996;108-110.
16. Urabe M., Hasumi Y., Ogasawara Y., Matsushita T., Kamoshita N., Nomoto A., Colosi P., Kurtzman G. J., Tobita K., Ozawa K. A novel dicistronic AAV vector using a short IRES segment derived from hepatitis C virus genome. Gene. 200:1997;157-162.
17. Sharma S., Miller P. W., Stolina M., Zhu L., Huang M., Paul R. W., Dubinett S. M. Multicomponent gene therapy vaccines for lung cancer: Effective eradication of established murine tumors in vivo with interleukin-7/herpes simplex thymidine kinase-transduced autologous tumor and ex vivo activated dendritic cells. Gene Ther. 4:1997;1361-1370.
18. Fan L., Drew J., Dunckley M. G., Owen J. S., Dickson G. Efficient coexpression and secretion of anti-atherogenic human apolipoprotein AI and lecithin-cholesterol acyltransferase by cultured muscle cells using adeno-associated virus plasmid vectors. Gene Ther. 5:1998;1434-1440.
19. Pizzato M., Franchin E., Calvi P., Boschetto R., Colombo M., Ferrini S., Palu G. Production and characterization of a bicistronic Moloney-based retroviral vector expressing human interleukin 2 and herpes simplex virus thymidine kinase for gene therapy of cancer. Gene Ther. 5:1998;1003-1007.
20. Okada H., Giezeman-Smits K. M., Tahara H., Attanucci J., Fellows W. K., Lotze M. T., Chambers W. H., Bozik M. E. Effective cytokine gene therapy against an intracranial glioma using a retrovirally transduced IL-4 plus HSVtk tumor vaccine. Gene Ther. 6:1999;219-226.
21. Niwa H., Yamamura K., Miyazaki J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene. 108:1991;193-200.
22. Mizuguchi H., Nakagawa T., Morioka Y., Imazu S., Nakanishi M., Kondo T., Hayakawa T., Mayumi T. Cytoplasmic gene expression system enhances the efficiency of cationic liposome-mediated in vivo gene transfer into mouse brain. Biochem. Biophys. Res. Commun. 234:1997;15-18.
23. Elroy-Stein O., Fuerst T. R., Moss B. Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5′ sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system. Proc. Natl. Acad. Sci. USA. 86:1989;6126-6130.
24. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 44:1986;283-292.
25. Kozak M. The scanning model for translation: An update. J. Cell Biol. 108:1989;229-241.
26. Liu F., Song Y. K., Liu D. Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA. Gene Ther. 6:1999;1258-1266.
27. Mizuguchi H., Nakagawa T., Nakanishi M., Imazu S., Nakagawa S., Mayumi T. Efficient gene transfer into mammalian cells using fusogenic liposome. Biochem. Biophys. Res. Commun. 218:1996;402-407.
28. Mizuguchi H., Nakagawa T., Yoyosawa S., Nakanishi M., Imazu S., Nakanishi T., Tsutsumi Y., Nakagawa S., Hayakawa T., Ijuhin N., Mayumi T. Tumor necrosis factor α-mediated tumor regression by the in vivo transfer of genes into the artery that leads to tumors. Cancer Res. 58:1998;5725-5730.
29. Moss B., Elroy-Stein O., Mizukami T., Alexander W. A., Fuerst T. R. New mammalian expression vectors. Nature. 348:1990;91-92.
30. Kozak M. Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Mol. Cell. Biol. 7:1987;3438-3445.
31. Miller A. D., Rosman G. J. Improved retroviral vectors for gene transfer and expression. BioTechniques. 9:1989;980-990.
32. Havenga M. J. E., Vogels R., Braakman E., Kroos N., Valerio D., Hagenbeek A., van Es H. H. G. Second gene expression in bicistronic constructs using short synthetic intercistrons and viral IRES sequences. Gene. 222:1998;319-327.
33. Li J., Samulski R. J., Xiao X. Role for highly regulated rep gene expression in adeno-associated virus vector production. J. Virol. 71:1997;5236-5243.
34. Chen L., Chen D., Block E., O'Donnell M., Kufe D. W., Clinton S. K. Eradication of murine bladder carcinoma by intratumor injection of a bicistronic adenoviral vector carrying cDNAs for the IL-12 heterodimer and its inhibition by the IL-12 p40 subunit homodimer. J. Immunol. 159:1997;351-359.