FMDV-2A sequence and protein arrangement contribute to functionality of CYP2B1-reporter fusion protein

Analytical Biochemistry

Volumn 343, Issue 1, 2005, Pages 116-124

  Lengler, Johannes ^a   Holzmüller, Harry ^a   Salmons, Brian ^a   Günzburg, Walter H ^a   Renner, Matthias ^a  

^a UNIVERSITY OF VETERINARY MEDICINE   (Austria)

Author keywords

CYP2B1; EGFP; FMDV 2A; Fusion protein; hrGFP; Reporter gene; Suicide gene

Indexed keywords

FLUORESCENCE; GENES; VIRUSES;

CYP2B1; EGFP; FMDV-2A; FUSION PROTEINS; HRGFP; REPORTER GENE;

PROTEINS;

AMINO ACID; CYTOCHROME P450 2B1; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELL STRAIN HEK293; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; FOOT AND MOUTH DISEASE VIRUS; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE;

FOOT-AND-MOUTH DISEASE VIRUS; RENILLA;

EID: 22144476888     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.05.004     Document Type: Article

References (23)

1. J. Wahlfors, S. Loimas, T. Pasanen, and T. Hakkarainen Green fluorescent protein (GFP) fusion constructs in gene therapy research Histochem. Cell Biol. 115 2001 59 65
2. A.B. Cubitt, R. Heim, S.R. Adams, A.E. Boyd, L.A. Gross, and R.Y. Tsien Understanding, improving and using green fluorescent proteins Trends Biochem. Sci. 20 1995 448 455
3. T.T. Yang, L. Cheng, and S.R. Kain Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein Nucleic Acids Res. 24 1996 4592 4593
4. H. Mao, K.S. Rosenthal, An N-terminal arginine-rich cluster and a proline-alanine-threonine repeat region determine the cellular localization of the herpes simplex virus type 1 ICP34.5 protein and its ligand, protein phosphatase 1, J. Biol. Chem. 277 (2002) 11423-11431. [Epub 12002 Jan 11411]
5. M. Prescott, S. Nowakowski, P. Nagley, and R.J. Devenish The length of polypeptide linker affects the stability of green fluorescent protein fusion proteins Anal. Biochem. 273 1999 305 307
6. M.L. Donnelly, G. Luke, A. Mehrotra, X. Li, L.E. Hughes, D. Gani, and M.D. Ryan Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip' J. Gen. Virol. 82 2001 1013 1025
7. A.L. Szymczak, C.J. Workman, Y. Wang, K.M. Vignali, S. Dilioglou, E.F. Vanin, D.A. Vignali, Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector, Nat. Biotechnol. 22 (2004) 589-594 [Epub 2004 Apr 2004]
8. P. de Felipe, L.E. Hughes, M.D. Ryan, J.D. Brown, Co-translational, intraribosomal cleavage of polypeptides by the foot-and-mouth disease virus 2A peptide, J. Biol. Chem. 278 (2003) 11441-11448 [Epub 12003 Jan 11408]
9. C.A. Brown, and S.D. Black Membrane topology of mammalian cytochromes P-450 from liver endoplasmic reticulum. Determination by trypsinolysis of phenobarbital-treated microsomes J. Biol. Chem. 264 1989 4442 4449
10. F.P. Guengerich, and A. Parikh Expression of drug-metabolizing enzymes Curr. Opin. Biotechnol. 8 1997 623 628
11. N.E. Sladek Metabolism of oxazaphosphorines Pharmacol. Ther. 37 1988 301 355
12. M.D. Burke, S. Thompson, C.R. Elcombe, J. Halpert, T. Haaparanta, and R.T. Mayer Ethoxy-, pentoxy- and benzyloxyphenoxazones and homologues: a series of substrates to distinguish between different induced cytochromes P-450 Biochem. Pharmacol. 34 1985 3337 3345
13. K.M. Kedzie, C.A. Balfour, G.Y. Escobar, S.W. Grimm, Y.A. He, D.J. Pepperl, J.W. Regan, J.C. Stevens, and J.R. Halpert Molecular basis for a functionally unique cytochrome P450IIB1 variant J. Biol. Chem. 266 1991 22515 22521
14. J. Hlavaty, A. Stracke, D. Klein, B. Salmons, W.H. Gunzburg, and M. Renner Multiple modifications allow high-titer production of retroviral vectors carrying heterologous regulatory elements J. Virol. 78 2004 1384 1392
15. I. Walter, M. Fleischmann, D. Klein, M. Muller, B. Salmons, W.H. Gunzburg, M. Renner, and W. Gelbmann Rapid and sensitive detection of enhanced green fluorescent protein expression in paraffin sections by confocal laser scanning microscopy Histochem. J. 32 2000 99 103
16. F.L. Graham, J. Smiley, W.C. Russell, and R. Nairn Characteristics of a human cell line transformed by DNA from human adenovirus type 5 J. Gen. Virol. 36 1977 59 74
17. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
18. E. Hartmann, T.A. Rapoport, and H.F. Lodish Predicting the orientation of eukaryotic membrane-spanning proteins Proc. Natl. Acad. Sci. USA 86 1989 5786 5790
19. R.J. Keenan, D.M. Freymann, R.M. Stroud, and P. Walter The signal recognition particle Annu. Rev. Biochem. 70 2001 755 775
20. E. Szczesna-Skorupa, C.D. Chen, S. Rogers, and B. Kemper Mobility of cytochrome P450 in the endoplasmic reticulum membrane Proc. Natl. Acad. Sci. USA 95 1998 14793 14798
21. M.D. Ryan, and J. Drew Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein EMBO J. 13 1994 928 933
22. T. Langer, S. Rosmus, and H. Fasold Intracellular localization of the 90 kDA heat shock protein (HSP90alpha) determined by expression of a EGFP-HSP90alpha-fusion protein in unstressed and heat stressed 3T3 cells Cell Biol. Int. 27 2003 47 52
23. http://www.stratagene.com/lit_items/BR55.pdf