Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors

BMC Neuroscience

Volumn 5, Issue , 2004, Pages

  Ahmed, Bushra Y ^a,b   Chakravarthy, Sridhara ^b   Eggers, Ruben ^b   Hermens, Wim T J M C ^b   Zhang, Jing Ying ^c   Niclou, Simone P ^b   Levelt, Christiaan ^b   Sablitzky, Fred ^c   Anderson, Patrick N ^a   Lieberman, A R ^b   Verhaagen, Joost ^b  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b NETHERLANDS INSTITUTE FOR NEUROSCIENCE   (Netherlands)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; PARVOVIRUS VECTOR; RECOMBINANT ENZYME;

AGE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN REGION; CELL LABELING; CELL LINE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOMEGALOVIRUS; DENTATE GYRUS; ENHANCER REGION; FLUORESCENCE ANALYSIS; FOREBRAIN; GENE CONTROL; GENE EXPRESSION; GENETIC RECOMBINATION; GENETIC STABILITY; GENETIC TRANSDUCTION; HIPPOCAMPUS; HUMAN; HUMAN CELL; IMMUNOREACTIVITY; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NEOCORTEX; NERVE CELL; NONHUMAN; PROMOTER REGION; REPORTER GENE; TOXICITY TESTING; TRANSGENE; VIRAL GENE DELIVERY SYSTEM; VIRUS INFECTION; VIRUS REPLICATION;

ANIMALS; CELL LINE; DEPENDOVIRUS; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HIPPOCAMPUS; INTEGRASES; LENTIVIRUS; LUMINESCENT PROTEINS; MICE; MICE, TRANSGENIC; NEOCORTEX; NEURONS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; TRANSGENES; VIRAL PROTEINS;

EID: 2642570238     PISSN: 14712202     EISSN: None     Source Type: Journal    
DOI: 10.1186/1471-2202-5-4     Document Type: Article

References (41)

1. Tsien JZ, Chen DF, Gerber D, Tom C, Mercer EH, Anderson DJ, Mayford M, Kandel ER, Tonegawa S: Subregion- and cell type restricted gene knockout in mouse brain. Cell 1996, 87:1317-1326.
2. Cinato E, Mirotsou M, Sablitzky F: Cre-mediated transgene activation in the developing and adult mouse brain. Genesis 2001, 31:118-125.
3. Silver DP, Livingston DM: Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity. Mol Cell 2001, 8:233-243.
4. Bueler H: Adeno-associated viral vectors for gene transfer and gene therapy. Biol Chem 1999, 380:613-622.
5. Naldini L: Lentiviruses as gene transfer agents for delivery to non-dividing cells. Curr Opin Biotechnol 1998, 9:457-463.
6. Zufferey R, Dull T, Mandel RJ, Bukovsky A, Quiroz D, Naldini L, Trono D: Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J Virol 1998, 72:9873-9880.
7. Blömer U, Naldini L, Kafri T, Trono D, Verma IM, Gage FH: Highly efficient and sustained gene transfer in adult neurons with a lentivirus vector. J Virol 1997, 71:6641-6649.
8. Xiao X, Li J, McCown TJ, Samulski RJ: Gene transfer by adenoassociated virus vectors into the central nervous system. Exp Neurol 1997, 144:113-124.
9. McCown TJ, Xiao X, Li J, Breese GR, Samulski RJ: Differential and persistent expression patterns of CNS gene transfer by an adeno-associated virus (AAV) vector. Brain Res 1996, 713:99-107.
10. Kaplitt MG, Leone P, Samulski RJ, Xiao X, Pfaff DW, O'Malley KL, During MJ: Long-term gene expression and phenotyping correction using adeno-associated virus vectors in the mammalian brain. Nat Genet 1994, 8:148-154.
11. Neut RVD: Targeted gene disruption: applications in neurobiology. J Neurosci Methods 1997, 71:19-27.
12. Abremski K, Hoess RH, Sternberg N: Studies on the properties of P1 site-specific recombination: Evidence for the topologically unlinked products following recombination. Cell 1983, 32:1301-1311.
13. Lakso M, Sauer B, Mosinger BJr, Lee EJ, Manning RW, Yu SH, Mulder KL, Westphal H: Targeted oncogene activation by site-specific recombination in transgenic mice. Proc Natl Acad Sci USA 1992, 89:6232-6236.
14. Zou YR, Gu H, Rajewsky K: Generation of a mouse strain that produces immunoglobulin kappa chains with human constant regions. Science 1993, 262:1271-1274.
15. Gu H, Zou YR, Rajewsky K: Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell 1993, 73:1155-1164.
16. Gu H, Marth JD, Orban PC, Mossmann H, Rajewsky K: Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 1994, 265:103-106.
17. Pfeifer A, Brandon EP, Kootstra N, Gage FH, Verma IM: Delivery of the Cre recombinase by a self deleting lentiviral vector: Efficient gene targeting in vivo. Proc Natl Acad Sci USA 2001, 98:11450-11455.
18. Loonstra A, Vooijs M, Beverloo HB, Allak BA, Drunen EV, Kanaar R, Berns A, Jonkers J: Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Proc Natl Acad Sci USA 2001, 98:9209-9214.
19. Schmidt EE, Taylor DS, Prigge JR, Barnet S, Capecchi R: Illegitimate Cre-dependent chromosome rearrangements in transgenic mouse spermatids. Proc Natl Acad Sci USA 2000, 97:13702-13707.
20. Sternberg N, Hamilton D, Hoess R: Bacteriophage P1 site-specific recombination. II. Recombination between loxP and the bacterial chromosome. J Mol Biol 1981, 150:487-507.
21. Sauer B: Identification of cryptic lox sites in the yeast genome by selection for Cre-mediated chromosome translocations that confer multiple drug resistance. J Mol Biol 1992, 223:911-928.
22. Sauer B: Multiplex Cre/lox recombination permits selective site-specific DNA targeting to both a natural and an engineered site in the yeast genome. Nucleic Acids Res 1996, 24:4608-4613.
23. Thyagarajan B, Guimaraes MJ, Groth AC, Calos MP: Mammalian genomes contain active recombinase recognition sites. Gene 2000, 244:47-54.
24. Soriano IP: Generalized lacZ expression with the Rosa26 Cre reporter strain. Nat Genet 1999, 21:70-71.
25. Kaspar BK, Vissel B, Bengoechea T, Crone S, Randolph-Moore L, Muller R, Brandon EP, Schaffer D, Verma IM, Lee KF, Heinemann SF, Gage FH: Adeno-associated virus effectively mediates conditional gene modification in the brain. Proc Natl Acad Sci USA 2002, 99:2320-2325.
26. Thévenot E, Côcé F, Colin P, He Y, Leblois H, Perricaudet M, Mallet J, Vodjdani G: Targeting conditional gene modification into the serotonin neurons of the dorsal raphe nucleus by viral delivery of the Cre recombinase. Mol Cell Neurosci in press.
27. Scammell TE, Arrigoni E, Thompson MA, Ronan PJ, Saper CB, Greene RW: Focal deletion of the adenosine A1 receptor in adult mice using an adeno-associated viral vector. J Neurosci 2003, 23:5762-70.
28. Prost S, Sheahan S, Rannie D, Harrison DJ: Adenovirus-mediated Cre deletion of floxed sequences in primary mouse cells is an efficient alternative for studies of gene deletion. Nucleic Acids Res 2001, 29:E80.
29. de Alboran IM, O'Hagan RC, Gartner F, Malynn B, Davidson L, Rickert R, Rajewsky K, DePinho RA, Alt FW: Analysis of C-MYC function in normal cells via conditional gene-targeted mutation. Immunity 2001, 14:45-55.
30. Smith RL, Traul DL, Schaack J, Clayton GH, Staley KJ, Wilcox CL: Characterization of promoter function and cell-type-specific expression from viral vectors in the nervous system. J Virol 2000, 74:11254-11261.
31. Acsadi G, Jani A, Massie B, Simoneau M, Holland P, Blaschuk K, Karpati GA: Differential efficiency of adenovirus-mediated in vivo gene transfer into skeletal muscle cells of different maturity. Hum Mol Genet 1994, 3:579-584.
32. Vincent-Lacaze N, Snyder RO, Gluzman R, Bohl D, Lagarde C, Danos O: Structure of adeno-associated virus vector DNA following transduction of the skeletal muscle. J Virol 1999, 73:1949-1955.
33. Doi K, Hargitai J, Kong J, Tsang SH, Wheatley M, Chang S, Goff S, Gouras P: Lentiviral transduction of green fluorescent protein in retinal epithelium: evidence of rejection. Vision Res 2002, 42:551-558.
34. Naldini L, Blömer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D: In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 1996, 272:263-267.
35. Follenzi A, Ailles LE, Bakovic S, Geuna M, Naldini L: Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-1 pol sequences. Nat Genet 2000, 25:217-222.
36. Tsien JZ, Chen DF, Gerber D, Tom C, Mercer EH, Anderson DJ, Mayford M, Kandel ER, Tonegawa S: Subregion- and cell type restricted gene knockout in mouse brain. Cell 1996, 87:1317-1326.
37. Grimm D, Kern A, Rittner K, Kleinschmidt JA: Novel tools for production and purification of recombinant adenoassociated virus vectors. Hum Gene Ther 1998, 9:2745-2760.
38. Hermens WTJMC, Brake OT, Dijkhuizen PA, Sonnemans MAF, Grimm D, Kleinschmidt JA, Verhaagen J: Purification of recombinant adeno-associated virus by iodixanol gradient ultracentrifugation allows rapid and reproducible preparation of vector stocks for gene transfer in the nervous system. Hum Gene Ther 1999, 10:1885-1891.
39. Naldini L, Blömer U, Gage FH, Trono D, Verma IM: Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci USA 1996, 93:11382-11388.
40. Paxinos G, Franklin KBJ: Atlas of Mouse brain, 2nd edition. Los Angeles 2001.
41. Hsu SM, Raine L, Fanger H: Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 1981, 29:577-580.