Zeb2 upregulates integrin α5 expression through cooperation with sp1 to induce invasion during epithelial-mesenchymal transition of human cancer cells

Carcinogenesis

Volumn 33, Issue 3, 2012, Pages 563-571

  Nam, Eun Hee ^a   Lee, Yunhee ^a,b   Park, Young Kyu ^c   Lee, Jung Weon ^d   Kim, Semi ^a,b  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^c Chonnam National University Medical School   (South Korea)

^d Seoul National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA5 INTEGRIN; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR SP1; TRANSCRIPTION FACTOR ZEB2; UNCLASSIFIED DRUG; UVOMORULIN; VIMENTIN;

ARTICLE; CANCER CELL; CANCER GROWTH; CELL INVASION; CONTROLLED STUDY; EMBRYO; EPITHELIUM CELL; GENE ACTIVATION; HUMAN; HUMAN CELL; MESENCHYME CELL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; TRANSCRIPTION REGULATION; UPREGULATION;

EID: 84863236117     PISSN: 01433334     EISSN: 14602180     Source Type: Journal    
DOI: 10.1093/carcin/bgs005     Document Type: Article

References (44)

1. Kalluri,R. et al. (2009) The basics of epithelial-mesenchymal transition. J. Clin. Invest., 119, 1420-1428.
2. Thiery,J.P. et al. (2006) Complex networks orchestrate epithelialmesenchymal transitions. Nat. Rev. Mol. Cell Biol., 7, 131-142.
3. Yang,J. et al. (2008) Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis. Dev. Cell, 14, 818-829.
4. Thiery,J.P. et al. (2009) Epithelial-mesenchymal transitions in development and disease. Cell, 139, 871-890.
5. Zeisberg,M. et al. (2009) Biomarkers for epithelial-mesenchymal transitions. J. Clin. Invest., 119, 1429-1437.
6. Cavallaro,U. et al. (2004) Cell adhesion and signalling by cadherins and Ig-CAMs in cancer. Nat. Rev. Cancer, 4, 118-132.
7. Vleminckx,K. et al. (1991) Genetic manipulation of E-cadherin expression by epithelial tumor cells reveals an invasion suppressor role. Cell, 66, 107-119.
8. Yang,J. et al. (2004) Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis. Cell, 117, 927-939.
9. Aigner,K. et al. (2007) The transcription factor ZEB1 (deltaEF1) promotes tumour cell dedifferentiation by repressing master regulators of epithelial polarity. Oncogene, 26, 6979-6988.
10. Kim,S. et al. (2010) TMPRSS4 induces invasion and epithelialmesenchymal transition through upregulation of integrin alpha5 and its signaling pathways. Carcinogenesis, 31, 597-606.
11. Peinado,H. et al. (2007) Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype? Nat. Rev. Cancer, 7, 415-428.
12. Comijn,J. et al. (2001) The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion. Mol. Cell, 7, 1267-1278.
13. Remacle,J.E. et al. (1999) New mode of DNA binding of multi-zinc finger transcription factors: deltaEF1 family members bind with two hands to two target sites. EMBO J., 18, 5073-5084.
14. Vandewalle,C. et al. (2005) SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions. Nucleic Acids Res., 33, 6566-6578.
15. Bindels,S. et al. (2006) Regulation of vimentin by SIP1 in human epithelial breast tumor cells. Oncogene, 25, 4975-4985.
16. Maschler,S. et al. (2005) Tumor cell invasiveness correlates with changes in integrin expression and localization. Oncogene, 24, 2032-2041.
17. Sheppard,D. (1996) Epithelial integrins. Bioessays, 18, 655-660.
18. Larjava,H. et al. (1993) Expression of integrins and basement membrane components by wound keratinocytes. J. Clin. Invest., 92, 1425-1435.
19. Gong,J. et al. (1997) Role of alpha 5 beta 1 integrin in determining malignant properties of colon carcinoma cells. Cell Growth Differ., 8, 83-90.
20. Kuwada,S.K. et al. (2005) Integrin alpha5/beta1 expression mediates HER-2 down-regulation in colon cancer cells. J. Biol. Chem., 280, 19027-19035.
21. Birkenmeier,T.M. et al. (1991) The alpha 5 beta 1 fibronectin receptor. Characterization of the alpha 5 gene promoter. J. Biol. Chem., 266, 20544-20549.
22. Corbi,A.L. et al. (2000) The alpha2 and alpha5 integrin genes: identification of transcription factors that regulate promoter activity in epidermal keratinocytes. FEBS Lett., 474, 201-207.
23. Koria,P. et al. (2007) KGF promotes integrin alpha5 expression through CCAAT/enhancer-binding protein-beta. Am. J. Physiol. Cell Physiol., 293, C1020-C1031.
24. Choi,S. et al. (2009) Cooperation between integrin alpha5 and tetraspan TM4SF5 regulates VEGF-mediated angiogenic activity. Blood, 113, 1845-1855.
25. Hagen,G. et al. (1994) Sp1-mediated transcriptional activation is repressed by Sp3. EMBO J., 13, 3843-3851.
26. Kim,S. et al. (2002) Inhibition of endothelial cell survival and angiogenesis by protein kinase A. J. Clin. Invest., 110, 933-941.
27. Jung,H. et al. (2008) TMPRSS4 promotes invasion, migration and metastasis of human tumor cells by facilitating an epithelial-mesenchymal transition. Oncogene, 27, 2635-2647.
28. Yates,B. et al. (2007) Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta. Exp. Cell Res., 313, 3718-3728.
29. Chamboredon,S. et al. (2005) v-Jun downregulates the alpha 2 (I) collagen target gene indirectly through Sp1/3. Oncogene, 24, 2547-2557.
30. Dignam,J.D. et al. (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res., 11, 1475-1489.
31. Verschueren,K. et al. (1999) SIP1, a novel zinc finger/homeodomain repressor, interacts with Smad proteins and binds to 5#-CACCT sequences in candidate target genes. J. Biol. Chem., 274, 20489-20498.
32. Alexander,N.R. et al. (2006) N-cadherin gene expression in prostate carcinoma is modulated by integrin-dependent nuclear translocation of Twist1. Cancer Res., 66, 3365-3369.
33. Taki,M. et al. (2006) Involvement of Ets-1 transcription factor in inducing matrix metalloproteinase-2 expression by epithelial-mesenchymal transition in human squamous carcinoma cells. Int. J. Oncol., 28, 487-496.
34. Ellenberger,T. et al. (1994) Crystal structure of transcription factor E47: E-box recognition by a basic region helix-loop-helix dimer. Genes Dev., 8, 970-980.
35. Black,A.R. et al. (2001) Sp1 and kruppel-like factor family of transcription factors in cell growth regulation and cancer. J. Cell. Physiol., 188, 143-160.
36. Li,L. et al. (2010) The role of Sp1 and Sp3 in normal and cancer cell biology. Ann. Anat., 192, 275-283.
37. Gingras,M.E. et al. (2003) Regulation of the integrin subunit alpha5 gene promoter by the transcription factors Sp1/Sp3 is influenced by the cell density in rabbit corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 44, 3742-3755.
38. Fuxe,J. et al. (2010) Transcriptional crosstalk between TGF-beta and stem cell pathways in tumor cell invasion: role of EMT promoting Smad complexes. Cell Cycle, 9, 2363-2374.
39. Jungert,K. et al. (2007) Sp1 is required for transforming growth factor-betainduced mesenchymal transition and migration in pancreatic cancer cells. Cancer Res., 67, 1563-1570.
40. Jorda,M. et al. (2005) Upregulation of MMP-9 in MDCKepithelial cell line in response to expression of the Snail transcription factor. J. Cell Sci., 118, 3371-3385.
41. Hu,C.T. et al. (2010) Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b. FEBS J., 277, 1202-1218.
42. Davidson,L.A. et al. (2006) Integrin alpha5beta1 and fibronectin regulate polarized cell protrusions required for Xenopus convergence and extension. Curr. Biol., 16, 833-844.
43. Qian,F. et al. (2005) Interaction between integrin alpha(5) and fibronectin is required for metastasis of B16F10 melanoma cells. Biochem. Biophys. Res. Commun., 333, 1269-1275.
44. Drake,J.M. et al. (2010) ZEB1 coordinately regulates laminin-332 and {beta}4 integrin expression altering the invasive phenotype of prostate cancer cells. J. Biol. Chem., 285, 33940-33948.