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Volumn 32, Issue 8, 2012, Pages 590-596

Experience with local lymph node assay performance standards using standard radioactivity and nonradioactive cell count measurements

Author keywords

Local lymph node assay; Lymph node cell counts; Non radioactive methods; Performance standards; Skin sensitisation

Indexed keywords

1 CHLORO 2,4 DINITROBENZENE; 2 MERCAPTOBENZOTHIAZOLE; 2 PROPANOL; BENZOIC ACID; BENZOIC ACID PHENYL ESTER; CHLOROBENZENE; CINNAMALDEHYDE; CINNAMYL ALCOHOL; CITRAL; COBALT CHLORIDE; DODECYL SULFATE SODIUM; ETHYLENE GLYCOL DIMETHACRYLATE; EUGENOL; HEXYLCINNAMAL; IMIDAZOLIDINYL UREA; ISOEUGENOL; LACTIC ACID; METHACRYLIC ACID METHYL ESTER; NICKEL CHLORIDE; PHENYLENEDIAMINE; SALICYLIC ACID; SALICYLIC ACID METHYL ESTER; UNCLASSIFIED DRUG; UREA; XYLENE;

EID: 84862899102     PISSN: 0260437X     EISSN: 10991263     Source Type: Journal    
DOI: 10.1002/jat.1684     Document Type: Article
Times cited : (30)

References (51)
  • 2
    • 0021331225 scopus 로고
    • Guinea pig maximization tests with formaldehyde releasers. Results from two laboratories
    • Andersen KE, Boman A, Hamann K, Wahlberg JE. 1984. Guinea pig maximization tests with formaldehyde releasers. Results from two laboratories. Contact Dermat. 10: 257-266.
    • (1984) Contact Dermat. , vol.10 , pp. 257-266
    • Andersen, K.E.1    Boman, A.2    Hamann, K.3    Wahlberg, J.E.4
  • 3
    • 33845536212 scopus 로고    scopus 로고
    • Information derived from sensitisation test methods: test sensitivity, false positives and false negatives
    • Basketter DA, Kimber I. 2007. Information derived from sensitisation test methods: test sensitivity, false positives and false negatives. Contact Dermat. 56: 1-4.
    • (2007) Contact Dermat. , vol.56 , pp. 1-4
    • Basketter, D.A.1    Kimber, I.2
  • 4
    • 85012853511 scopus 로고    scopus 로고
    • Contact hypersensitivity
    • 2nd edn, McQueen CA (ed.),. Elsevier: Oxford
    • Basketter DA, Kimber I. 2010. Contact hypersensitivity. In Comprehensive Toxicology, 2nd edn, McQueen CA (ed.), Vol. 5. Elsevier: Oxford; 397-411.
    • (2010) Comprehensive Toxicology , vol.5 , pp. 397-411
    • Basketter, D.A.1    Kimber, I.2
  • 5
    • 0032489787 scopus 로고    scopus 로고
    • Strategies for identifying false positive responses in predictive sensitisation tests
    • Basketter DA, Gerberick GF, Kimber I. 1998. Strategies for identifying false positive responses in predictive sensitisation tests. Food Chem. Toxicol. 36: 327-333.
    • (1998) Food Chem. Toxicol. , vol.36 , pp. 327-333
    • Basketter, D.A.1    Gerberick, G.F.2    Kimber, I.3
  • 6
    • 34447295889 scopus 로고    scopus 로고
    • The local lymph node assay EC3 value: status of validation
    • Basketter DA, Gerberick GF, Kimber I. 2007a. The local lymph node assay EC3 value: status of validation. Contact Dermat. 57: 70-75.
    • (2007) Contact Dermat. , vol.57 , pp. 70-75
    • Basketter, D.A.1    Gerberick, G.F.2    Kimber, I.3
  • 7
    • 36749027674 scopus 로고    scopus 로고
    • Does irritation potency contribute to the skin sensitisation potency of contact allergens?
    • Basketter DA, Kan-King-Yu D, Dierkes P, Jowsey IR. 2007b. Does irritation potency contribute to the skin sensitisation potency of contact allergens? J. Cut. Ocular Toxicol, 26: 279-286.
    • (2007) J. Cut. Ocular Toxicol , vol.26 , pp. 279-286
    • Basketter, D.A.1    Kan-King-Yu, D.2    Dierkes, P.3    Jowsey, I.R.4
  • 10
    • 33746910539 scopus 로고    scopus 로고
    • Skin sensitisation potency of methyl methacrylate in the local lymph node assay: comparisons with guinea pig data and human experience
    • Betts CJ, Dearman RJ, Heylings JR, Kimber I, Basketter DA. 2006. Skin sensitisation potency of methyl methacrylate in the local lymph node assay: comparisons with guinea pig data and human experience. Contact Dermat. 55: 140-147.
    • (2006) Contact Dermat. , vol.55 , pp. 140-147
    • Betts, C.J.1    Dearman, R.J.2    Heylings, J.R.3    Kimber, I.4    Basketter, D.A.5
  • 12
    • 0001267196 scopus 로고
    • Delayed contact hypersensitivity in the guinea pig
    • Buehler EV. 1965. Delayed contact hypersensitivity in the guinea pig. Arch. Dermatol. 91: 171-177.
    • (1965) Arch. Dermatol. , vol.91 , pp. 171-177
    • Buehler, E.V.1
  • 14
    • 0035697810 scopus 로고    scopus 로고
    • ICCVAM evaluation of the murine local lymph node assay. II. Conclusions and recommendations of an independent scientific peer review panel
    • Dean JH, Twerdok LE, Tice RR, Sailstad DM, Hattan DG, Stokes WS. 2001. ICCVAM evaluation of the murine local lymph node assay. II. Conclusions and recommendations of an independent scientific peer review panel. Regul. Toxicol. Pharmacol. 34: 258-273.
    • (2001) Regul. Toxicol. Pharmacol. , vol.34 , pp. 258-273
    • Dean, J.H.1    Twerdok, L.E.2    Tice, R.R.3    Sailstad, D.M.4    Hattan, D.G.5    Stokes, W.S.6
  • 16
    • 84862834352 scopus 로고    scopus 로고
    • ECHA. Guidance on Information Requirements and Chemical Safety Assessment. Chapter R.7a: Endpoint specific guidance. European Chemicals Agency: Helsinki
    • ECHA. 2008. Guidance on Information Requirements and Chemical Safety Assessment. Chapter R.7a: Endpoint specific guidance. European Chemicals Agency: Helsinki; 256-288.
    • (2008) , pp. 256-288
  • 17
    • 84862883680 scopus 로고    scopus 로고
    • ECVAM. Murine Local Lymph Node Assay Performance Standards. European Centre for the Validation of Alternative Methods, October 2008; available from: (accessed 12 January 2011).
    • ECVAM. 2008. Murine Local Lymph Node Assay Performance Standards. European Centre for the Validation of Alternative Methods, October 2008; available from: (accessed 12 January 2011).
    • (2008)
  • 20
    • 80052424295 scopus 로고    scopus 로고
    • Skin sensitisation: local lymph node assay (LLNA)
    • EU B42
    • EU B42. 2004. Skin sensitisation: local lymph node assay (LLNA) Official J. Eur. Comm. L 152.
    • (2004) Official J. Eur. Comm.
  • 21
    • 56249092468 scopus 로고    scopus 로고
    • Local lymph node assay (LLNA): comparison of different protocols by testing skin sensitizing epoxy resin components
    • Gamer AO, Nies E, Vohr H-W. 2008. Local lymph node assay (LLNA): comparison of different protocols by testing skin sensitizing epoxy resin components. Regul. Toxicol. Pharmacol. 52: 290-298.
    • (2008) Regul. Toxicol. Pharmacol. , vol.52 , pp. 290-298
    • Gamer, A.O.1    Nies, E.2    Vohr, H.-W.3
  • 24
    • 0032927419 scopus 로고    scopus 로고
    • Development of a non-radioactive endpoint in a modified local lymph node assay
    • Hariya T, Hatao M, Ichikawa H. 1999. Development of a non-radioactive endpoint in a modified local lymph node assay. Food Chem. Toxicol. 37: 87-93.
    • (1999) Food Chem. Toxicol. , vol.37 , pp. 87-93
    • Hariya, T.1    Hatao, M.2    Ichikawa, H.3
  • 25
    • 0028917236 scopus 로고
    • A modification of the local lymph node assay for contact allergenicity screening: measurement of interleukin-2 as an alternative to radioisotope-dependent proliferation assay
    • Hatao M, Hariya T, Katsumura Y, Kato S. 1995. A modification of the local lymph node assay for contact allergenicity screening: measurement of interleukin-2 as an alternative to radioisotope-dependent proliferation assay. Toxicology 98: 15-22.
    • (1995) Toxicology , vol.98 , pp. 15-22
    • Hatao, M.1    Hariya, T.2    Katsumura, Y.3    Kato, S.4
  • 26
    • 0038324413 scopus 로고    scopus 로고
    • Assessment of cumulative allergen-activated lymph node cell proliferation using flow cytometry
    • Humphreys N, Dearman R, Kimber I. 2003. Assessment of cumulative allergen-activated lymph node cell proliferation using flow cytometry. Toxicol. Sci. 73: 80-89.
    • (2003) Toxicol. Sci. , vol.73 , pp. 80-89
    • Humphreys, N.1    Dearman, R.2    Kimber, I.3
  • 27
    • 84862891377 scopus 로고    scopus 로고
    • ICCVAM. Appendix B: Comparative LLNA, Guinea Pig, and Human Data Used in the Performance Evaluation; available from: (accessed 21 January 2011).
    • ICCVAM. 2008. Appendix B: Comparative LLNA, Guinea Pig, and Human Data Used in the Performance Evaluation; available from: (accessed 21 January 2011).
    • (2008)
  • 28
    • 84862883679 scopus 로고    scopus 로고
    • ICCVAM. ICCVAM Evaluation of Nonradioactive Versions of the Murine Local Lymph Node Assay; available from: (accessed 12 January 2011).
    • ICCVAM. 2010. ICCVAM Evaluation of Nonradioactive Versions of the Murine Local Lymph Node Assay; available from: (accessed 12 January 2011).
    • (2010)
  • 29
    • 45849105158 scopus 로고    scopus 로고
    • The impact of vehicle on the relative potency of skin sensitising chemicals in the local lymph node assay
    • Jowsey IR, Clapp CJ, Safford B, Gibbons BT, Basketter DA. 2008. The impact of vehicle on the relative potency of skin sensitising chemicals in the local lymph node assay. Food Chem. Toxicol. 27: 67-75.
    • (2008) Food Chem. Toxicol. , vol.27 , pp. 67-75
    • Jowsey, I.R.1    Clapp, C.J.2    Safford, B.3    Gibbons, B.T.4    Basketter, D.A.5
  • 30
    • 0026504266 scopus 로고
    • The murine local lymph node assay; collaborative studies and new directions: A commentary
    • Kimber I, Basketter DA. 1992. The murine local lymph node assay; collaborative studies and new directions: A commentary. Food Chem Toxicol. 30: 165-169.
    • (1992) Food Chem Toxicol. , vol.30 , pp. 165-169
    • Kimber, I.1    Basketter, D.A.2
  • 32
    • 43049183580 scopus 로고    scopus 로고
    • Comparison of the skin sensitizing potential of unsaturated compounds and assessed by the murine local lymph node assay (LLNA) and the guinea pig maximization test (GPMT)
    • Kreiling R, Hollnagel HM, Hareng L, Eigler D, Lee MS, Griem P, Dreesen B, Kleber M, Albrecht A, Garcia C, Wendel A. 2008. Comparison of the skin sensitizing potential of unsaturated compounds and assessed by the murine local lymph node assay (LLNA) and the guinea pig maximization test (GPMT). Food Chem. Toxicol. 46: 1896-1904.
    • (2008) Food Chem. Toxicol. , vol.46 , pp. 1896-1904
    • Kreiling, R.1    Hollnagel, H.M.2    Hareng, L.3    Eigler, D.4    Lee, M.S.5    Griem, P.6    Dreesen, B.7    Kleber, M.8    Albrecht, A.9    Garcia, C.10    Wendel, A.11
  • 33
    • 0038731369 scopus 로고    scopus 로고
    • A nonradioisotopic endpoint for measurement of lymph node cell proliferation in a murine allergic contact dermatitis model, using bromodeoxyuridine immunohistochemistry
    • Lee J, Park J, Park S, Kim H, Oh H. 2002. A nonradioisotopic endpoint for measurement of lymph node cell proliferation in a murine allergic contact dermatitis model, using bromodeoxyuridine immunohistochemistry. J. Pharmacol. Toxicol. Meth. 48: 53-61.
    • (2002) J. Pharmacol. Toxicol. Meth. , vol.48 , pp. 53-61
    • Lee, J.1    Park, J.2    Park, S.3    Kim, H.4    Oh, H.5
  • 36
    • 28344457410 scopus 로고    scopus 로고
    • OECD. Paris: Organization for Economic Cooperation and Development.
    • OECD. 2002. Test Guideline 429: The Local Lymph Node Assay. Paris: Organization for Economic Cooperation and Development.
    • (2002) Test Guideline 429: The Local Lymph Node Assay
  • 37
    • 84862855204 scopus 로고    scopus 로고
    • OECD. Paris: Organization for Economic Cooperation and Development.
    • OECD. 2010a. Test Guideline 429: The Local Lymph Node Assay. Paris: Organization for Economic Cooperation and Development.
    • (2010) Test Guideline 429: The Local Lymph Node Assay
  • 38
    • 84862868486 scopus 로고    scopus 로고
    • OECD. Paris: Organisation for Economic Cooperation and Development.
    • OECD. 2010b. Guidelines for Test of Chemicals No. 442a and 442b. Paris: Organisation for Economic Cooperation and Development.
    • (2010) Guidelines for Test of Chemicals No. 442a and 442b
  • 39
    • 0025304219 scopus 로고
    • A review of the Buehler guinea pig skin sensitization test and its use in a risk assessment process for human skin sensitization
    • Robinson MK, Nusair TL, Fletcher ER, Ritz HL. 1990. A review of the Buehler guinea pig skin sensitization test and its use in a risk assessment process for human skin sensitization. Toxicology 61: 91-107.
    • (1990) Toxicology , vol.61 , pp. 91-107
    • Robinson, M.K.1    Nusair, T.L.2    Fletcher, E.R.3    Ritz, H.L.4
  • 42
    • 0035826075 scopus 로고    scopus 로고
    • Development of non-radio isotopic endpoint of murine local lymph node assay, based on 5-bromo-2'-deoxyuridine (BrdU) incorporation
    • Takeyoshi M, Yamasaki K, Yakabe Y, Takatsuki M, Kimber I. 2001. Development of non-radio isotopic endpoint of murine local lymph node assay, based on 5-bromo-2'-deoxyuridine (BrdU) incorporation. Toxicol. Lett. 119: 203-208.
    • (2001) Toxicol. Lett. , vol.119 , pp. 203-208
    • Takeyoshi, M.1    Yamasaki, K.2    Yakabe, Y.3    Takatsuki, M.4    Kimber, I.5
  • 43
    • 0142056980 scopus 로고    scopus 로고
    • Assessment of statistical analysis in non-radioisotopic local lymph node assay (non-RI-LLNA) with alpha-hexylcinnamic aldehyde as an example
    • Takeyoshi M, Sawaki M, Yamasaki K, Kimber I. 2003. Assessment of statistical analysis in non-radioisotopic local lymph node assay (non-RI-LLNA) with alpha-hexylcinnamic aldehyde as an example. Toxicology 30: 259-263.
    • (2003) Toxicology , vol.30 , pp. 259-263
    • Takeyoshi, M.1    Sawaki, M.2    Yamasaki, K.3    Kimber, I.4
  • 44
    • 16844378142 scopus 로고    scopus 로고
    • Novel approach for classifying chemicals according to skin sensitizing potency by non-radioisotopic modification of the local lymph node assay
    • Takeyoshi M, Iida K, Shiraishi K, Hoshuyama S. 2005. Novel approach for classifying chemicals according to skin sensitizing potency by non-radioisotopic modification of the local lymph node assay. J. Appl. Toxicol. 25: 129-134.
    • (2005) J. Appl. Toxicol. , vol.25 , pp. 129-134
    • Takeyoshi, M.1    Iida, K.2    Shiraishi, K.3    Hoshuyama, S.4
  • 45
    • 31644443469 scopus 로고    scopus 로고
    • Advantage of using CBA/N strain mice in a non-readioisotopic modification of the local lymph node assay
    • Takeyoshi M, Noda S, Yamasaki K, Kimber I. 2006. Advantage of using CBA/N strain mice in a non-readioisotopic modification of the local lymph node assay. J. Appl. Toxicol. 26: 5-9.
    • (2006) J. Appl. Toxicol. , vol.26 , pp. 5-9
    • Takeyoshi, M.1    Noda, S.2    Yamasaki, K.3    Kimber, I.4
  • 46
    • 84862891376 scopus 로고    scopus 로고
    • United Nations. Globally Harmonized System of Classification and Labelling of Chemicals (GHS). Part 3: Health Hazards; available from:
    • United Nations. 2009. Globally Harmonized System of Classification and Labelling of Chemicals (GHS). Part 3: Health Hazards; available from:
    • (2009)
  • 47
    • 84862847140 scopus 로고    scopus 로고
    • Skin sensitization - revised final health effects test guideline
    • US EPA.
    • US EPA. 2003. Skin sensitization - revised final health effects test guideline. Fed. Reg. 68(58): 14635-14636.
    • (2003) Fed. Reg. , vol.68 , Issue.58 , pp. 14635-14636
  • 48
    • 28444479129 scopus 로고    scopus 로고
    • The local lymph node assay being too sensitive?
    • Vohr H-V, Jurgen AH. 2005. The local lymph node assay being too sensitive? Arch. Toxicol. 79: 721-728.
    • (2005) Arch. Toxicol. , vol.79 , pp. 721-728
    • Vohr, H.-V.1    Jurgen, A.H.2
  • 49
    • 0033627813 scopus 로고    scopus 로고
    • An intra-laboratory validation of the Integrated Model for the Differentiation of Skin Reactions (IMDS): discrimination between (photo)allergic and (photo)irritant skin reactions in mice
    • Vohr H-W, Blumel J, Blotz A, Homey B, Ahr HJ. 2000. An intra-laboratory validation of the Integrated Model for the Differentiation of Skin Reactions (IMDS): discrimination between (photo)allergic and (photo)irritant skin reactions in mice. Arch. Toxicol. 73: 501-509.
    • (2000) Arch. Toxicol. , vol.73 , pp. 501-509
    • Vohr, H.-W.1    Blumel, J.2    Blotz, A.3    Homey, B.4    Ahr, H.J.5
  • 50
    • 0032406387 scopus 로고    scopus 로고
    • A combined murine local lymph node and irritancy assay to predict sensitization and irritancy potential of chemicals
    • Woolhiser MR, Hayes BB, Meade BJ. 1998. A combined murine local lymph node and irritancy assay to predict sensitization and irritancy potential of chemicals. Toxicol. Meth. 8: 245-256.
    • (1998) Toxicol. Meth. , vol.8 , pp. 245-256
    • Woolhiser, M.R.1    Hayes, B.B.2    Meade, B.J.3
  • 51
    • 43749120288 scopus 로고    scopus 로고
    • Development of a modified local lymph node assay using ATP measurement as an endpoint
    • Yamashita K, Idehara K, Fukuda N, Yamagishi G, Kawada N. 2005. Development of a modified local lymph node assay using ATP measurement as an endpoint. AATEX 11: 136-144.
    • (2005) AATEX , vol.11 , pp. 136-144
    • Yamashita, K.1    Idehara, K.2    Fukuda, N.3    Yamagishi, G.4    Kawada, N.5


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