Phloroglucinol: Antioxidant properties and effects on cellular oxidative markers in human HepG2 cell line

Food and Chemical Toxicology

Volumn 50, Issue 8, 2012, Pages 2886-2893

  Quéguineur, Benoît ^a   Goya, Luis ^b   Ramos, Sonia ^b   Martín, Maria Angeles ^b   Mateos, Raquel ^b   Bravo, Laura ^b  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^b INSTITUTE OF FOOD SCIENCE   (Spain)

Author keywords

Antioxidant defences; Antioxidants; Marine bioactive compounds; Oxidative stress; Phloroglucinol

Indexed keywords

GLUTATHIONE; GLUTATHIONE PEROXIDASE; GLUTATHIONE TRANSFERASE; MALONALDEHYDE; PHLOROGLUCINOL; PHLOROTANNIN DERIVATIVE; REACTIVE OXYGEN METABOLITE; SCAVENGER; TANNIN DERIVATIVE; TROLOX C; UNCLASSIFIED DRUG;

ANTIOXIDANT ACTIVITY; ARTICLE; BIOLOGICAL ACTIVITY; CELL PROTECTION; CELL STRAIN HEPG2; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; LIPID PEROXIDATION; OXIDATIVE STRESS; STANDARD;

ANTIOXIDANTS; BIOLOGICAL MARKERS; CELL LINE; HUMANS; OXIDATIVE STRESS; PHLOROGLUCINOL;

EMBRYOPHYTA;

EID: 84862675001     PISSN: 02786915     EISSN: 18736351     Source Type: Journal    
DOI: 10.1016/j.fct.2012.05.026     Document Type: Article

References (30)

1. Alía M., Ramos S., Mateos R., Bravo L., Goya L. Response of the antioxidant defense system to tert-butylhydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2). J. Biochem. Mol. Toxicol. 2005, 19:119-128.
2. Alía M., Mateos R., Ramos S., Lecumberri E., Bravo L., Goya L. Influence of quercetin and rutin on growth and antioxidant defense system of a human hepatoma cell line (HepG2). Eur. J. Nutr. 2006, 45:19-28.
3. Bae J.-S. Antithrombotic and profibrinolytic activities of phloroglucinol. Food Chem. Toxicol. 2011, 49:1572-1577.
4. Barwell C.J., Blunden G., Manandhar P.D. Isolation and characterization of brown algal polyphenols as inhibitors of α-amylase, lipase and trypsin. J. Appl. Phycol. 1989, 1:319-323.
5. Benzie I.F.F., Strain J.J. The ferric reducing ability of plasma (FRAP) as a measure of " antioxidant power": the FRAP assay. Anal. Biochem. 1996, 239:70-76.
6. Connan S., Goulard F., Stiger V., Deslandes E., Ar Gall E. Interspecific and temporal variation in phlorotannin levels in an assemblage of brown algae. Botanica Marina 2004, 47:410-416.
7. Dollo G., Chevanne F., Le Corre P., Chemtob C., Le Verge R. Biodisponibilité du phloroglucinol chez l'Homme. J. Pharm. Belgique 1999, 54:75-82.
8. Fujie K., Ito H. Distribution and excretion of 1,3,5-trihydroxybenzene. Arzneim. Forsch. 1972, 22:777-780.
9. Goya L., Delgado-Andrade C., Rufián-Henares J.A., Bravo L., Morales F.J. Effect of coffee Melanoidin on human hepatoma HepG2 cells. Protection against oxidative stress induced by tert-butylhydroperoxide. Mol. Nutr. Food Res. 2007, 51:536-545.
10. Granado-Serrano A.B., Angeles Martín M., Izquierdo-Pulido M., Goya L., Bravo L., Ramos S. Molecular mechanisms of (-)-epicatechin and chlorogenic acid on the regulation of the apoptotic and survival/proliferation pathways in a human hepatoma cell line. J. Agric. Food Chem. 2007, 55:2020-2027.
11. Guiry M.D., Blunden G. Seaweed Resources in Europe: Uses and Potential 1991, John Wiley and Sons, Chichester.
12. Hissin P.J., Hilf R. A fluorimetric method for determination of oxidized and reduced glutathione in tissues. Anal. Biochem. 1976, 74:214-226.
13. Hodnick W.F., Milosavljevic E.B., Nelson J.H., Pardini R.S. Electrochemistry of flavonoids: relationships between redox potentials, inhibition of mitochondrial respiration, and production of oxygen radicals by flavonoids. Biochem. Pharmacol. 1988, 37:2607-2611.
14. Kang K.A., Lee K.H., Chae S., Zhang R., Jung M.S., Ham Y.M., Baik J.S., Lee N.H., Hyun J.W. Cytoprotective effect of phloroglucinol on oxidative stress induced cell damage via catalase activation. J. Cell. Biochem. 2006, 97:609-620.
15. Kang K.A., Zhang R., Chae S., Lee S.J., Kim J., Kim J., Jeong J., Lee J., Shin T., Lee N.H., Hyun J.W. Phloroglucinol (1,3,5-trihydroxybenzene) protects against ionizing radiation-induced cell damage through inhibition of oxidative stress in vitro and in vivo. Chem. Biol. Interact. 2010, 185:215-226.
16. Kim H., Roh H., Lee H.J., Chung S.Y., Choi S.O., Lee K.R., Han S.B. Determination of phloroglucinol in human plasma by high-performance liquid chromatography, mass spectrometry. J. Chrom. B 2003, 792:307-312.
17. Kim M.-M., Kim S.-K. Effect of phloroglucinol on oxidative stress and inflammation. Food Chem. Toxicol. 2010, 48:2925-2933.
18. Martín M.A., Ramos S., Mateos R., Izquierdo-Pulido M., Bravo L., Goya L. Protection of human HepG2 cells against oxidative stress by the flavonoid epicatechin. Phytother. Res. 2010, 24:503-509.
19. Mateos R., Goya L., Bravo L. Determination of malondialdehyde by liquid chromatography as the 2,4-dinitrophenylhydrazone derivative: a marker for oxidative stress in cell cultures of human hepatoma HepG2. J. Chrom. B 2004, 805:33-39.
20. Mateos R., Goya L., Bravo L. Metabolism of the olive oil phenols hydroxytyrosol, tyrosol, and hydroxytyrosyl acetate by human hepatoma HepG2 cells. J. Agric. Food Chem. 2005, 53:9897-9905.
21. Myhrstad M.C., Carlsen H., Nordstrom O., Blomhoff R., Moskaug J.O. Flavonoids increase the intracellular glutathione level by transactivation of the γ-glutamylcysteine synthetase catalytical subunit promoter. Free Radical Biol. Med. 2002, 32:386-393.
22. Pilz J., Meineke I., Gleiter C.H. Measurement of free and bound malondialdehyde in plasma by high-performance liquid chromatography as the 2,4-dinitrophenylhydrazine derivative. J. Chrom. B: Biomed. Sci. Appl. 2000, 742:315-325.
23. Pulido R., Bravo L., Saura-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J. Agric. Food Chem. 2000, 48:3396-3402.
24. Ragan M.A., Glombitza K.-W. Phlorotannins, brown algal polyphenols. Progress Phycol. Res. 1986, 4:129-241.
25. Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol. Med. 1999, 26:1231-1237.
26. Scharf G., Prustomersky S., Knasmuller S., Schulte-Hermann R., Huber W.W. Enhancement of glutathione and γ-glutamylcysteine synthetase, the rate limiting enzyme of glutathione synthesis, by chemoprotective plant-derived food and beverage components in the human hepatoma cell line HepG2. Nutr. Cancer 2003, 45:74-83.
27. Singh I.P., Sidana J., Bansal P., Foley W.J. Phloroglucinol compounds of therapeutic interest: global patent and technology status. Expert Opin. Therap. Patents 2009, 19:847-866.
28. Singh I.P., Sidana J., Bharate S.B., Foley W.J. Phloroglucinol compounds of natural origin: synthetic aspects. Nat. Prod. Rep. 2010, 27:393-416.
29. Wang H., Joseph J.A. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radical Biol. Med. 1999, 27:612-616.
30. Zubia M., Fabre M.S., Kerjean V., Lann K.L., Stiger-Pouvreau V., Fauchon M., Deslandes E. Antioxidant and antitumoural activities of some Phaeophyta from Brittany coasts. Food Chem. 2009, 116:693-701.